HvLBD19基因对大麦不定根发育的调控

doi:10.3724/SP.J.1006.2022.11088

作物学报 ›› 2022, Vol. 48 ›› Issue (10): 2435-2442.doi: 10.3724/SP.J.1006.2022.11088

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

HvLBD19基因对大麦不定根发育的调控

郭宝健¹^,²(), 王爽¹^,², 吕超¹^,², 王菲菲¹^,², 许如根¹^,²^,^*()

¹江苏省作物基因组学和分子育种重点实验室 / 植物功能基因组学教育部重点实验室 / 江苏省作物遗传生理重点实验室江苏扬州 225009
²扬州大学农学院 / 江苏省粮食作物现代产业技术协同创新中心, 江苏扬州 225009

收稿日期:2021-10-12 接受日期:2022-02-25 出版日期:2022-10-12 网络出版日期:2022-03-22
通讯作者: 许如根
作者简介:第一作者联系方式: E-mail: bjguo@yzu.edu.cn
基金资助:
国家自然科学基金项目(31771771);江苏省高等学校自然科学研究重大项目(19KJA560005);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05);江苏高校优势学科建设工程项目资助

Regulation of adventitious root development by HvLBD19 gene in barley (Hordeum vulgare L.)

GUO Bao-Jian¹^,²(), WANG Shuang¹^,², LYU Chao¹^,², WANG Fei-Fei¹^,², XU Ru-Gen¹^,²^,^*()

¹Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding / Key Laboratory of Plant Functional Genomics of the Ministry of Education / Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, Jiangsu, China
²Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China

Received:2021-10-12 Accepted:2022-02-25 Published:2022-10-12 Published online:2022-03-22
Contact: XU Ru-Gen
Supported by:
National Natural Science Foundation of China(31771771);Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJA560005);China Agriculture Research System of MOF and MARA(CARS-05);and the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘要/Abstract

摘要：

植物LBD基因家族是植物特异的转录因子家族, 在调控植物生长发育和氮素代谢方面起到重要的作用。基因组共线性分析表明, 大麦(Hordeum vulgare L.) HvLBD19基因为水稻ARL1和玉米RTCS基因直系同源基因。基因时空表达分析结果表明, 该基因在不定根中表达丰度最高, 且受外源生长素诱导表达, 所编码蛋白定位于细胞核内。转基因功能验证结果表明, 苗期超表达株系相对于野生型不定根长度增长近1倍, 不定根数目增加40%。本研究初步明确了HvLBD19基因影响大麦不定根发育, 为进一步深入探究大麦HvLBD19基因调控不定根发育的分子机制提供了基础。

关键词: 大麦, 侧生器官边界域基因, 超表达, 不定根

Abstract:

LBD gene family is a plant specific transcription factor family, which plays an important role in regulating plant development and nitrogen metabolism. Micro-synteny analysis showed that barley HvLBD19 was an orthologous of maize RTCS and rice ARL1 gene. Temporal and spatial expression analysis revealed that this gene was most abundant in adventitious roots and was induced by exogenous auxin treatment, which encoded a protein located in the nucleus. Transgenic results indicated that the adventitious root length increased by nearly one time, and the number of adventitious roots increased by 40%. It was preliminarily confirmed that HvLBD19 gene controlled adventitious root development in barley and provided a foundation for further research on the molecular mechanism of HvLBD19 gene in regulating adventitious root development in barley.

Key words: barley, LATERAL ORGAN BOUNDARIES DOMAIN gene, overexpression, adventitious root

郭宝健, 王爽, 吕超, 王菲菲, 许如根. HvLBD19基因对大麦不定根发育的调控[J]. 作物学报, 2022, 48(10): 2435-2442.

GUO Bao-Jian, WANG Shuang, LYU Chao, WANG Fei-Fei, XU Ru-Gen. Regulation of adventitious root development by HvLBD19 gene in barley (Hordeum vulgare L.)[J]. Acta Agronomica Sinica, 2022, 48(10): 2435-2442.

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引物编号 Primer pairs ID	引物序列 Primer sequence (5′-3′)	内容 Content
P1	ATGACGGGACTGGGTTCGCC	编码区扩增 Coding sequence amplification
P1	TTACGAGCGATTAAGGTAAG	编码区扩增 Coding sequence amplification
P2	AGTTTCTGCGTCGCAAGTG	荧光定量 qRT-PCR
P2	GAGCTTGGAGACGTTGCTG	荧光定量 qRT-PCR
P3	GGTCCATCCTAGCCTCACTC	内参基因HvActin扩增 Internal control of HvActin amplification
P3	GATAACAGCAGTGGAGCGCT	内参基因HvActin扩增 Internal control of HvActin amplification
P4	TCTCCCTTACCCATGATGAGCATGACGGGACTGGG	瞬时表达载体构建 Transient expression vector construction
P4	CACGGGGGACTCTAGCGAGCGATTAAGGTAAGCATACGC	瞬时表达载体构建 Transient expression vector construction
P5	CCCAAGCTTCTTGCATGCCTGCAGTGCAGCGTGACCCG	Ubi启动子扩增 Ubi promoter amplification
P5	CCCCCCGGGCTGCAGAAGTAACACCAAAC	Ubi启动子扩增 Ubi promoter amplification
P6	GACTAGTGAGCTCGAATTTCCCCGATC	nos终止子扩增 nos terminator amplification
P6	GCTCTAGAGATCTGATATCATCGATGAA	nos终止子扩增 nos terminator amplification
P7	CCCCCCGGGATGACGGGACTGGGTTCGCC	大麦超表达载体构建 Overexpression vector construction in barley
P7	GACTAGTTTACGAGCGATTAAGGTAAG	大麦超表达载体构建 Overexpression vector construction in barley

HvLBD19基因对大麦不定根发育的调控

Regulation of adventitious root development by HvLBD19 gene in barley (Hordeum vulgare L.)

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