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作物学报

作物学报 ›› 2022, Vol. 48 ›› Issue (10): 2443-2450.doi: 10.3724/SP.J.1006.2022.14188

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用中国抗源区分强致病力大豆胞囊线虫群体的探讨

练云(), 魏荷, 王金社, 张辉, 雷晨芳, 李金英, 卢为国()   

  1. 河南省作物分子育种研究院 / 国家大豆改良中心郑州分中心 / 农业农村部黄淮海油料作物重点实验室 / 河南省油料作物遗传改良重点实验室, 河南郑州 450002
  • 收稿日期:2021-10-17 接受日期:2022-01-05 出版日期:2022-10-12 网络出版日期:2022-07-20
  • 通讯作者: 卢为国
  • 作者简介:第一作者联系方式: E-mail: lianyun262@126.com
  • 基金资助:
    国家重点研发计划项目(2017YFD0101400)

Identification highly virulent population of soybean cyst nematode using China germplasms

LIAN Yun(), WEI He, WANG Jin-She, ZHANG Hui, LEI Chen-Fang, LI Jin-Ying, LU Wei-Guo()   

  1. Henan Academy of Crop Molecular Breeding / Zhengzhou Subcenter of National Soybean Improvement Center / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture and Rural Affairs / Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, Henan, China
  • Received:2021-10-17 Accepted:2022-01-05 Published:2022-10-12 Published online:2022-07-20
  • Contact: LU Wei-Guo
  • Supported by:
    National Key Research and Development Program of China(2017YFD0101400)

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摘要：

由大豆胞囊线虫(Soybean cyst nematode, Heterodera glycines, SCN)引起的病害是一种世界性大豆病害。随着强致病力大豆胞囊线虫群体X12的出现及LY1线虫群体的合成, 国际通用的Riggs和HG type 2种大豆胞囊线虫生理小种鉴别模式已不能将4号生理小种、X12线虫群体、LY1线虫群体有效区分, 本研究提供了一种区分这3个线虫群体的简易鉴定方法, 为SCN相关研究提供技术支撑。包括以下步骤: 利用感病品种在病土中繁殖备用接种的胞囊; 用兴县灰皮支(ZDD2315)和PI567516C作为鉴别寄主, Lee为感病对照, 接种鉴定; 若兴县灰皮支和PI567516C均表现感病, 表明该病土感染的是X12线虫群体; 若兴县灰皮支表现抗病而PI567516C表现感病, 表明该病土感染的是4号生理小种; 若PI567516C表现抗病, 表明该病土感染的是LY1线虫群体。以上结果表明, 利用我国优异抗源兴县灰皮支和PI567516C 作为鉴别寄主, 能有效区分目前报道的具有强致病力的大豆胞囊线虫4号生理小种、X12和LY1线虫群体。本研究结果对筛选抗源、调查大豆胞囊线虫生理小种分布、线虫致病基因研究有重要意义。

关键词: 大豆胞囊线虫, 生理小种, 强致病力, 抗源

Abstract:

The disease caused by soybean cyst nematode (soybean cyst nematode, Heterodera glycines, SCN) is a worldwide soybean disease. The internationally used race identification models including Riggs model and Hg type for differentiate soybean cyst nematode population could not effectively distinguish the highly virulent race such as race 4, X12 population, and synthesized nematode population LY1. This study provides a simple identification method for distinguishing the three highly virulent population of the soybean cyst nematode, which would be greatly contribute to SCN research. This method included the following steps: First, we used the susceptible varieties and raised enough cysts in the soil infected highly virulent population of SCN for inoculation. Then, soybean varieties Xingxianhuipizhi (ZDD2315) and PI567516C were selected as differential hosts, and Lee was used as susceptible control for inoculation. Both ZDD2315 and PI567516C was sensitive in the inoculating reaction, indicating that the soil was infected by X12 population. If ZDD2315 was resistance while PI567516C were susceptible to the disease, indicating the soil was infected by race 4. If PI567516C was resistance to disease, it revealed that the soil was infected by LY1 population. These results indicated that the three highly virulent population including race 4, X12 population, and synthesized LY1 population could be effectively distinguished depending on the reaction of SCN on germplasms of ZDD2315 and PI567516C from China. In conclusion, these results of this study are of great significance for screening resistance germplasms, investigating the race distribution of SCN, and studying the pathogenic genes of SCN.

Key words: soybean cyst nematode, race, highly virulent, germplasms

表1

X12线虫群体和4号生理小种接种Riggs模式鉴别寄主"

寄主
Differential host		 接种X12线虫群体 Inoculating X12 population 接种4号生理小种 Inoculating race 4 接种LY1线虫群体* Inoculating LY1 population*
每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction		 每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction		 每株上的平均胞囊数目
Average cysts number per plant		 胞囊指数
FI		 抗性反应
Reaction
Lee 185.4±26.9 (144-214) 330.6±62.8 (237-401) 100
Pickett 181.6±87.7 (52-264) 98 + 310.6±64.0 (251-413) 94 + /
Peking 95.2±91.5 (25-206) 51.3 + 292.0±21.3 (270-318) 88.3 + / 83 +
PI88788 177.6±94.1 (103-339) 95.8 + 290.0±93.8 (143-384) 87.7 + / 45 +
PI90763 93.0±47.9 (26-133) 50.2 + 99.6±21.0 (78-127) 30.1 + / 92 +

表2

X12线虫群体和4号生理小种接种HG type模式鉴别寄主"

寄主
Differential host		 接种X12线虫群体Inoculating X12 population 接种4号生理小种 Inoculating race 4 接种LY1线虫群体* Inoculating LY1 population*
每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction		 每株上的平均胞囊数目**
Average number of cysts per plant**		 胞囊指数
FI		 抗性反应 Reaction 每株上的平均胞囊数目
Average cysts number per plant		 胞囊指数
FI		 抗性反应
Reaction
Lee 185.4±26.9 (144-214) / 330.6±62.8 (237-401) / 100
Peking 95.2±91.5 (25-206) 51.3 + 292.0±21.3 (270-318) 88.3 + / 83 +
PI88788 177.6±94.1 (103-339) 95.8 + 290.0±93.8 (143-384) 87.7 + / 45 +
PI90763 93.0±47.9 (26-133) 50.2 + 99.6±21.0 (78-127) 30.1 + / 92 +
PI437654 51.2±23.1 (11-66) 27.6 + 23.6±6.4 (17-34) 7.1 - / 260 +
PI89772 179.5±51.5 (122-239) 96.8 + 54.0±5.7 (50-58) 16.3 + / 172 +
PI209332 235.0±46.7 (181-282) 126.8 + 421.2±86.8 (344-532) 127.4 + / 55 +
PI548316 224.8±49.2 (156-270) 121.2 + 251.8±98.6 (141-394) 76.2 + / 130 +

表3

利用ZDD2315和PI567516C区分强致病力大豆胞囊线虫群体的鉴定"

寄主
Differential		 接种X12线虫群体Inoculating X12 population 接种4号生理小种 Inoculating race 4 接种LY1线虫群体* Inoculating LY1 population*
每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction		 每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction		 每株上的平均胞囊数目**
Average cysts number per plant**		 胞囊指数
FI		 抗性反应
Reaction
Lee 185.4±26.9 (144-214) / 330.6±62.8 (237-401) / 100
ZDD2315 145.4±87.7 (52-264) 87.7 + 14.0±2.8 (12-16) 4.2 - /
PI567516C 225±51.5 (122-239) 121.4 + 80.2±32.8 (53-135) 24.2 + 7 -

表4

利用ZDD2315和PI567516C验证多个土样来源的4号、2号生理小种和X12线虫群体"

生理小种
Race		 生理小种鉴定Race testing 抗性反应Reaction 土样编号
Soil code		 土样来源 Origin of the soil 土样采集时间
Soil sample collecting time (month/day)		 土样采集地点
Soil sample collecting location
Lee* PI567516C ZDD2315 经度
Longitude (°)		 纬度
Latitude (°)
X12 185.4±26.9 (144-214) + + SX12 112.166 37.844 2012/7 山西省邢家社乡想儿岭村
Xiangerling village, Xingjiashe township, Shanxi province
X12 118.4±28.7 (62-158) + + GJ30 112.257 38.014 2019/8 山西省河口镇段家岭村
Duanjialing village, Hekou town, Shanxi province
X12 135±38.7 (85-176) + + GJ07 112.166 37.844 2019/8 山西省邢家社乡想儿岭村
Xiangerling village, Xingjiashe township, Shanxi province
X12 222.9±36.2 (159-265) + + SX12单胞囊繁殖
SX12 Raised from single cyst		 112.166 37.844 2016/1 河南省农业科学院培育
Cultivated from Henan Academy of Agricultural Sciences
Race 4 122.4±23.2 (97-154) + - GJ05 112.190 37.758 2019/8 山西省邢家社乡郭家社村
Guojiashe village, Xingjiashe township, Shanxi province
Race 4 156.3±33.1 (118-192) + - GJ21 111.967 37.846 2019/8 山西省马兰镇南龙沟村
Nanlonggou village, Malan town, Shanxi province
Race 4 189±42.5 (129-229) + - GJ27 112.218 37.998 2019/8 山西省河口镇大坡村
Dapo village, Hekou town, Shanxi province
Race 4 110.4±21 (78-143) + - GJ14 112.070 37.876 2019/8 山西省马兰镇姬家庄村
Jijiazhuang village, Malan town, Shanxi province
Race 4 134±62.2 (45-260) + - GJ28 112.232 38.017 2019/8 山西省河口镇水头村
Shuitou village, Hekou town, Shanxi province
Race 4 124.6±26.1 (87-165) + - SX37 113.232 38.477 2012/7 山西省忻州市定襄县南庄村
Nanzhuang village, Dingxiang county, Xinzhou city, Shanxi province
Race 4 168±26.6 (135-212) + - SX49 112.802 39.484 2012/7 山西省朔州市山阴县安荣乡
An Rongxiang, Shanyin county, Shuozhou city, Shanxi province
Race 4 220±42.8 (138-270) + - SX51 112.468 39.511 2012/7 山西省朔州市平鲁区李西沟村
Lixigou village, Pinglu district, Shuozhou city, Shanxi province
Race 4 187.1±33.9 (130-222) + - SX59 112.613 36.935 2012/7 山西省长治市沁县西汤村
Xitang village, Qin county, Changzhi city, Shanxi province
Race 4 137.1±36 (90-196) + - SX37 113.232 38.477 2012/7 山西省忻州市定襄县南庄村
Nanzhuang village, Dingxiang county, Xinzhou city, Shanxi province
Race 2 170.4±50.6 (106-252) + - GJ 08 111.960 37.761 2019/8 山西省常安乡小娄峰村
Xiao Loufeng village, Chang'an township, Shanxi province
Race 2 95±32 (43-127) + - GJ 02 112.161 37.860 2019/8 山西省桃园办事处李家社村
Lijiashe village, Taoyuan office, Shanxi province
Race 2 659±283.5 (297-1138) + - GJ 04 112.188 37.785 2019/8 山西省邢家社乡龙子村
Longzi village, Xingjiashe township, Shanxi province
Race 2 286±80.5 (164-397) + - GJ 12 112.05 37.816 2019/8 山西省常安乡麻家口村
Majiakou village, Chang'an township, Shanxi province
Race 2 242.3±113 (74-454) + - GJ 16 111.924 37.861 2019/8 山西省岔口乡板四沟村
Bansigou village, Chakou township, Shanxi province
Race 2 112.3±44.7 (41-166) + - GJ 21 111.967 37.846 2019/8 山西省马兰镇南龙沟村
Nanlonggou village, Malan town, Shanxi province
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