作物学报 ›› 2015, Vol. 41 ›› Issue (07): 1064-1072.doi: 10.3724/SP.J.1006.2015.01064
司二静1,2,张宇1,2,汪军成1,2,孟亚雄1,2,李葆春1,3,马小乐1,2,尚勋武2,王化俊1,2,*
SI Er-Jing12,ZHANG Yu12,WANG Jun-Cheng12,MENG Ya-Xiong1,2,LI Bao-Chun1,3,MA Xiao-Le12,SHANG Xun-Wu2,WANG Hua-Jun1,2,*
摘要:
为了解大麦亲本材料遗传特性和主要农艺性状特征,采用156份不同来源的大麦材料,在86个多态性SSR位点上检测遗传多样性,同时对7个农艺性状在两试验点作表型鉴定,利用GLM和MLM模型进行分子标记与表型性状的关联分析。结果共检测出392个等位变异,平均每个标记4.6个,PIC值变异范围为0.0612~0.8560。群体遗传结构分析将156份材料分为2个亚群。利用GLM模型分析结果表明,与株高、穗长、芒长、穗粒数和千粒重5个性状相关联的标记有18个,单个标记对表型变异的解释率为4.81%~20.75%;利用MLM模型分析,与株高、穗长、芒长、分蘖数、穗粒数和千粒重6个性状相关联的标记有14个,单个标记对表型变异的解释率范围为6.64%~31.55%。这些关联标记对后续研究有参考价值。
| [1]Tanksley S D, McCouch S R. Seed banks and molecular maps: unlocking genetic potential from the wild. Science, 1997, 277: 1063–1066[2]Flint-Garcia S A, Thornsberry J M, Buckler I V. Structure of linkage disequilibrium in plants. Annu Rev Plant Biol, 2003, 54: 357–374[3]Kruglyak L. Prospects for whole-genome linkage disequilibrium mapping of common disease genes. Nat Genet, 1999, 22: 139–144[4]Jorde L B. Linkage disequilibrium and the search for complex disease genes. Genome Res, 2000, 10: 1435–1444[5]魏添梅, 昌小平, 闵东红, 景蕊莲. 小麦抗旱品种的遗传多样性分析及株高优异等位变异挖掘. 作物学报, 2010, 36: 895–904Wei T M, Chang X P, Min D H, Jing R L. Analysis of genetic diversity and tapping elite alleles for plant height in drought-tolerant wheat varieties. Acta Agron Sin, 2010, 36: 895–904 (in Chinese with English abstract) [6]Maccaferri M, Sanguineti M C, Noli E, Tuberosa R. Population structure and long-range linkage disequilibrium in a durum wheat elite collection. Mol Breed, 2005, 15: 271–290[7]Liu S, Yang X, Zhang D, Bai G, Chao S, Bockus W. Genome-wide association analysis identified SNPs closely linked to a gene resistant to soil-borne wheat mosaic virus. Theor Appl Genet, 2014, 127: 1039–1047[8]Ducrocq S, Madur D, Veyrieras J B, Camus-Kulandaivelu L, Kloiber-Maitz M, Presterl T, Ouzunova M, Manicacci D, Charcosset A. Key impact of Vgt1 on flowering time adaptation in maize: evidence from association mapping and ecogeographical information. Genetics, 2008, 178: 2433–2437[9]Kumar B, Abdel-Ghani A H, Pace J, Reyes-Matamoros J, Hochholdinger F, Lübberstedt T. Association analysis of single nucleotide polymorphisms in candidate genes with root traits in maize (Zea mays L.) seedlings. Plant Sci, 2014, 224: 9–19[10]Wen W, Li D, Li X, Gao Y, Li W, Li H, Liu J, Liu H, Chen W, Luo J. Metabolome-based genome-wide association study of maize kernel leads to novel biochemical insights. Nat Commun, 2014, 5:3438, DOI: 10.1038/ncomms4438 (2014)[11]Eizenga G C, Agrama H A, Lee F N, Yan W, Jia Y. Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Sci, 2006, 46: 1870-1878[12]Yonemaru J, Mizobuchi R, Kato H, Yamamoto T, Yamamoto E, Matasubara K, Hirabayashi H, Takeuchi Y, Tsunematsu H, Ishii T. Genomic regions involved in yield potential detected by genome-wide association analysis in Japanese high-yielding rice cultivars. BMC Genomics, 2014, 15: 346[13]D'hoop B B, Keizer P L, Paulo M J, Visser R G, van Eeuwijk F A, van Eck H J. Identification of agronomically important QTL in tetraploid potato cultivars using a marker-trait association analysis. Theor Appl Genet, 2014, 127: 731–748[14]Ivandic V, Hackett C A, Nevo E, Keith R, Thomas W T, Forster B P. Analysis of simple sequence repeats (SSRs) in wild barley from the Fertile Crescent: associations with ecology, geography and flowering time. Plant Mol Biol, 2002, 48: 511–527[15]Wu D, Qiu L, Xu L, Ye L, Chen M, Sun D, Chen Z, Zhang H, Jin X, Dai F, Zhang, G. Genetic variation of HvCBF genes and their association with salinity tolerance in Tibetan annual wild barley. PLoS One, 2011, 6: e22938[16]Cai S, Wu D, Jabeen Z, Huang Y, Huang Y, Zhang G. Genome-wide association analysis of aluminum tolerance in cultivated and tibetan wild barley. PloS One, 2013, 8: e69776[17]Visioni A, Tondelli A, Francia E, Pswarayi A, Malosetti M, Russell J, Thomas William, Waugh R, Pecchioni N, Romagosa I. Genome-wide association mapping of frost tolerance in barley (Hordeum vulgare L.). BMC Genomics, 2013, 14: 424[18]Xia Y, Li R, Ning Z, Bai G, Siddique K H M, Yan G, Baum M, Varshney R K, Guo P. Single nucleotide polymorphisms in HSP17.8 and their association with agronomic traits in barley. PloS One, 2013, 8: e56816[19]Ivandic V, Thomas W T B, Nevo E, Zhang Z, Forster B P. Associations of simple sequence repeats with quantitative trait variation including biotic and abiotic stress tolerance in Hordeum spontaneum. Plant Breed, 2003, 122: 300–304[20]Kraakman A T, Niks R E, Van den Berg P M, Stam P, Van Eeuwijk F A. Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics, 2004, 168: 435–446[21]Kraakman A T, Niks R E, Van den Berg P M, Stam P, Van Eeuwijk F A. Linkage disequilibrium mapping of morphological, resistance, and other agronomically relevant traits in modern spring barley cultivars. Mol Breed, 2006, 17: 41–58[22]Roy J K, Smith K P, Muehlbauer G J, Chao S, Close T J, Steffenson B J. Association mapping of spot blotch resistance in wild barley. Mol Breed, 2010, 26: 243–256[23]赖勇, 王鹏喜, 范贵强, 司二静, 王晋, 杨轲, 孟亚雄, 李葆春, 马小乐, 尚勋武, 王化俊. 大麦SSR标记遗传多样性及其与农艺性状关联分析, 中国农业科学, 2013, 46: 233–242Lai Y, Wang P X, Fan G Q, Si E J, Wang J, Yang K, Meng Y X, Li B C, Ma X L, Shang X W, Wang H J. Genetic Diversity and association analysis using ssr marker in barley. Sci Agric Sin, 2013, 46: 233–242 (in Chinese with English abstract)[24]Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep, 1997, 15: 8–15[25]Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep, 1993, 11: 122–127[26]Korff M, Wang H, Leon J, Pillen K. Development of candidate introgression lines using an exotic barley accession ( Hordeum vulgare ssp. spontaneum) as donor. Theor Appl Genet, 2004, 109: 1736–1745[27]Breseghello F, Sorrells M E. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics, 2006, 172: 1165–1177[28]Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 2005, 14: 2611-2620[29]Kline J B, Moore D J, Clevenger C V. Activation and association of the Tec tyrosine kinase with the human prolactin receptor: mapping of a Tec/Vav1-receptor binding site. Mol Endocrinol, 2001, 15: 832–841[30]Harris B P, Stokesbury K D E. The spatial structure of local surficial sediment characteristics on Georges Bank, USA. Cont Shelf Res, 2010, 30: 1840–1853[31]Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: present status and future prospects. Plant Mol Biol, 2005, 57: 461–485[32]魏世平, 刘晓芬, 杨胜先, 吕海燕, 牛远, 章元明. 中国栽培大豆群体结构不同分类方法的比较. 南京农业大学学报, 2011, 34: 13–17Wei S P, Liu X F, Yang S X, Lü H Y, Niu Y, Zhang Y M. Comparison of various clustering methods for population structure in Chinese cultivated soybean [Glycine max (L.) Merr.]. J Nanjing Agric Univ, 2011, 34(2): 13–17 (in Chinese with English abstract)[33]文自翔, 赵团结, 郑永战, 刘顺湖, 王春娥, 王芳, 盖钧镒.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析: I. 群体结构及关联标记. 作物学报, 2008, 34: 1169–1178Wen Z X, Zhao T J, Zheng Y Z, Liu S H, Wang C E, Wang F, Gai J Y. Association analysis of agronomic and quality traits with ssr markers in Glycine max and Glycine soja in China: I. Population structure and associated markers. Acta Agron Sin, 2008, 34: 1169–1178 (in Chinese with English abstract)[34]Pillen K, Zacharias A, Léon J. Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum. Theor Appl Genet, 2004, 108: 1591–1601[35]Pillen K, Zacharias A, Léon J. Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet, 2003, 107: 340–352[36]Inostroza L, del Pozo A, Matus I, Castillo D, Hayes P, Machado S, Corey A. Association mapping of plant height, yield, and yield stability in recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Mol Breed, 2009, 23: 365–376[37]Locatelli A, Cuesta-Marcos A, Gutiérrez L, Hayes P M, Smith K P, Castro A J. Genome-wide association mapping of agronomic traits in relevant barley germplasm in Uruguay. Mol Breed, 2013, 31: 631–654[38]Tondelli A, Xu X, Moragues M, Sharma R, Schnaithmann F, Ingvardsen C, Manninen O, Comadran J, Russell J, Waugh R. Structural and temporal variation in genetic diversity of European spring two-row barley cultivars and association mapping of quantitative traits. Plant Genome, 2013, 6: 1–14[39]von Korff M, Wang H, Le´on J, Pillen K. AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum). Theor Appl Genet, 2006, 112: 1221–1231[40]Hori K, Kobayashi T, Shimizu A, Sato K, Takeda K, Kawasaki S. Efficient construction of high-density linkage map and its application to QTL analysis in barley. Theor Appl Genet, 2003, 107: 806–813[41]Baghizadeh A, Taleei A R, Naghavi M R. QTL analysis for some agronomic traits in barley (Hordum vulgare L.). Int J Agric Biol, 2007, 9: 372–374[42]Sameri M, Takeda K, Komatsuda T. Quantitative trait loci controlling agronomic traits in recombinant inbred lines from a cross of oriental-and occidental-type barley cultivars. Breed Sci, 2006, 56: 243–252[43]Shahinnia F, Druka A, Franckowiak J, Morgante M, Waugh R, Stein N. High resolution mapping of dense spike-ar (dsp. ar) to the genetic centromere of barley chromosome 7H. Theor Appl Genet, 2012, 124: 373–384[44]Wang J, Yang J, McNeil D L, Zhou M. Identification and molecular mapping of a dwarfing gene in barley (Hordeum vulgare L.) and its correlation with other agronomic traits. Euphytica, 2010, 175: 331–342[45]Lex J, Ahlemeyer J, Friedt W, Ordon F. Genome-wide association studies of agronomic and quality traits in a set of German winter barley (Hordeum vulgare L.) cultivars using Diversity Arrays Technology (DArT). J Appl Genet, 2014, 1–11[46]Kalladan R, Worch S, Rolletschek H, Harshavardhan V T, Kuntze L, Seiler C, Sreenivasulu, N, Röder M S. Identification of quantitative trait loci contributing to yield and seed quality parameters under terminal drought in barley advanced backcross lines. Mol Breed, 2013, 32: 71–90[47]Teulat, B, Merah O, Souyris I, This D. QTLs for agronomic traits from a Mediterranean barley progeny grown in several environments. Theor Appl Genet, 2001, 103: 774–787[48]Ellis R P, Forster B P, Gordon D C, Handley L L, Keith R P, Lawrence P, Mever R, Powell W, Robinson D, Scrimgeour C M. Phenotype/genotype associations for yield and salt tolerance in a barley mapping population segregating for two dwarfing genes. J Exp Bot, 2002, 53: 1163–1176[49]Baum M, Grando S, Backes G, Jahoor A, Sabbagh A, Ceccarelli S. QTLs for agronomic traits in the Mediterranean environment identified in recombinant inbred lines of the cross 'Arta' × H. spontaneum 41-1. Theor Appl Genet, 2003, 107: 1215–1225 |
| [1] | 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590. |
| [2] | 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372. |
| [3] | 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400. |
| [4] | 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072. |
| [5] | 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789. |
| [6] | 牛丽, 王勇胜, 王长杰, 张宏, 孟亚雄, 李葆春, 杨轲, 马小乐, 姚立蓉, 司二静, 王化俊, 汪军成. 大麦NAC基因家族鉴定分析及HvNAC38的耐盐功能验证[J]. 作物学报, 2026, 52(3): 688-707. |
| [7] | 李赢, 石晓旭, 刘海翠, 石吕, 薛亚光, 魏亚凤. 裸大麦籽粒β-葡聚糖含量近红外预测模型的建立[J]. 作物学报, 2026, 52(3): 735-745. |
| [8] | 李瑞, 余意雯, 王敦亮, 田婷, 孙灵湘, 陶玥玥, 孙华. 长江中下游油菜薹油兼用模式菜籽产量特征比较研究[J]. 作物学报, 2026, 52(2): 620-630. |
| [9] | 鲁雅妮, 丁超杰, 张煜, 杜习军, 齐学礼, 胡琳, 许为钢. 河南省200份小麦品种苗期茎基腐病抗性鉴定与全基因组关联分析[J]. 作物学报, 2026, 52(2): 363-375. |
| [10] | 李诗晴, 王茜, 王素华, 张耀文, 王丽侠. 绿豆种质资源苗期耐盐性鉴定及相关基因发掘[J]. 作物学报, 2026, 52(2): 376-388. |
| [11] | 余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432. |
| [12] | 迟晓元, 刘庆, 张君, 赵旭红, 李美, 于天一, 潘丽娟, 许静, 姜骁, 殷祥贞, 马俊卿, 陈娜. 不同花生品种(系)耐盐碱性田间鉴定及各性状指标相关性研究[J]. 作物学报, 2026, 52(1): 85-98. |
| [13] | 杨锐, 陈敬东, 黄郢, 张学昆, 周登文, 刘清云, 徐劲松, 谢伶俐, 许本波. 基于北纬30°分界的长江中游油菜增产策略研究[J]. 作物学报, 2026, 52(1): 99-117. |
| [14] | 李云香, 郭千纤, 侯万伟, 张小娟. 引进ICARDA小麦苗期根系抗旱性状的全基因组关联分析[J]. 作物学报, 2025, 51(9): 2387-2398. |
| [15] | 李璐琪, 程宇坤, 白斌, 雷斌, 耿洪伟. 小麦叶片气孔相关性状全基因组关联分析[J]. 作物学报, 2025, 51(9): 2266-2284. |
|
||