作物学报 ›› 2017, Vol. 43 ›› Issue (12): 1746-1759.doi: 10.3724/SP.J.1006.2017.01746
胡德益1,蔡露1,陈光登1,*,张锡洲1,刘春吉2
HU De-Yi1, CAI Lu1, CHEN Guang-Deng1,*, ZHANG Xi-Zhou1,Chunji LIU2
摘要:
磷素营养与大麦品质及产量密切相关,磷高效遗传机制和品种改良是近年的研究热点之一。本研究利用由大麦栽培品种Baudin和种质材料CN4079杂交构建的重组自交系(RIL)群体,低磷胁迫(0.02 mmol L1 KH2PO4)与正常供磷(0.2 mmol L1 KH2PO4)条件下,对地上部和地下部磷素利用效率、磷素吸收效率和干重,以及分蘖数相关的QTL定位,并预测相关位点基因。表型鉴定结果表明,各性状在RIL群体中表现连续变异,并存在超亲分离。两种磷水平下,共检测到16个QTL,分布在2H、3H和5H染色体上,表型贡献率14.1%~28.5%。3H染色体上含有3个磷素利用效率位点,其增效等位基因均来源于Baudin,其中Qspue.sau-3H.1和Qrpue.sau-3H与控制磷素吸收效率的Qspae.sau-3H和Qrpae.sau-3H处于同一区段,而Qspue.sau-3H.2与控制分蘖数的位点Qtn.sau-3H处于同一区段。5H染色体上含有3个磷素吸收效率位点,其中Qspae.sau-5H.2和Qrpae.sau-5H的增效等位基因来自CN4079,且与控制磷素利用效率的Qspue.sau-5H和Qrpue.sau-5H,以及控制干重的Qsdw.sau-5H和Qrdw.sau-5H处于同一区段。在磷效率相关的4个区段中,除Qspue.sau-3H.1所处区间仅含有磷酸代谢与磷脂代谢相关基因外,其他区间均包含磷酸盐转运蛋白基因、磷酸代谢与磷脂代谢相关基因。
| [1]Schachtman D, Reid R, Ayling S M. Phosphorus uptake by plants: from soil to cell. Plant Physiol, 1998, 116: 447–453 [2]Hermans C, Hammond J P, White P J, Verbruggen N. How do plants respond to nutrient shortage by biomass allocation? Trends Plant Sci, 2006, 11: 610–617 [3]Hammond J P, White P J. Sucrose transport in the phloem: integrating root responses to phosphorus starvation. J Exp Bot, 2008, 59: 93–109 [4]唐宏亮, 申建波, 张福锁, Rengel Z. 磷和外源生长素对白羽扇豆(Lupinus albus L.)根形态和生理特性的影响. 中国科学C辑: 生命科学, 2013, 43: 201–212 Tang H L, Shen J B, Zhang F S, Rengel Z. Interactive effects of phosphorus deficiency and exogenous auxin on root morphological and physiological traits in white lupin (Lupinus albus L.). Sci Sin Vitae, 2013, 43: 201–212 (in Chinese) [5]赵静, 付家兵, 廖红, 何勇, 年海, 胡月明, 邱丽娟, 董英山, 严小龙. 大豆磷效率应用核心种质的根构型性状评价. 科学通报, 2004, 49: 1249–1257 Zhao J, Fu J B, Liao H, He Y, Nian H, Hu Y M, Dong Y S, Yan X L. The traits evaluation about root architecture of soybean core collection’s efficiency of phosphorus application. Chin Sci Bull, 2004, 49: 1249–1257 (in Chinese) [6]Tiessen H. Phosphorus in the global environment: transfers, cycles and management. Oceanographic Literature Review, 1995: 27–42 [7]郭程瑾, 李宾兴, 王斌, 李雁鸣, 肖凯. 小麦高效吸收和利用磷素的生理机制. 作物学报, 2006, 32: 827–832 Guo C J, Li B X, Wang B, Li Y M, Xiao K. Physiological mechanisms of absorption and use of phosphorus with high efficiency in wheat cultivars. Acta Agron Sin, 2006, 32: 827–832 (in Chinese with English abstract) [8]韦还和, 孟天瑶, 李超, 张洪程, 戴其根, 马荣荣, 王晓燕, 杨筠文. 水稻甬优12产量13.5 t hm-2以上超高产群体的氮素积累、分配与利用特征. 作物学报, 2016, 42: 886–897 Wei H H, Meng T Y, Li C, Zhang H C, Dai Q G, Ma R R, Wang X Y, Yang J W. Accumulation, distribution, and utilization characteristics of phosphorus in Yongyou 12 yielding over 13.5 t ha–1. Acta Agron Sin, 2016, 42: 886–897 (in Chinese with English abstract) [9]蔡秋燕, 张锡洲, 李廷轩, 陈光登, 吴德勇. 磷高效野生大麦拔节期对植酸态有机磷的利用. 中国农业科学, 2015, 48: 3146–3155 Cai Q Y, Zhang X Z, Li T X, Chen G D, Wu D Y. The utilization of phytate organic phosphorus in P-efficient wild barley genotypes at jointing stage. Sci Agric Sin, 2015, 48: 3146–3155 (in Chinese with English abstract) [10]林海建, 张志明, 张永中, 高世斌, 潘光堂. 作物氮、磷、钾利用相关性状的QTL定位研究进展. 植物营养与肥料学报, 2010, 16: 732–743 Lin H J, Zhang Z M, Zhang Y Z, Gao S B, Pan G T. Advancement of QTL analysis for traits associated to N, P and K utilization. Plant Nutr Fert Sci, 2010, 16: 732–743 (in Chinese with English abstract) [11]Wissuwa M, Yano M, Ae N. Mapping of QTLs for phosphorus-deficiency tolerance in rice (Oryza sativa L.). Theor Appl Genet, 1998, 97: 777–783 [12]Chin J H, Lu X, Haefele S M, Gamuyao R, Ismail A, Wissuwa M. Development and application of gene-based markers for the major rice QTL Phosphorus uptake 1. Theor Appl Genet, 2010, 120: 1073–1086 [13]Li Y D, Wang Y J, Tong Y P, Gao J G, Zhang J S, Chen S Y. QTL mapping of phosphorus deficiency tolerance in soybean (Glycine max L. Merr.). Euphytica, 2005, 142: 137–142 [14]King K E, Lauter N, Lin S F, Scott M P, Shoemaker R C. Evaluation and QTL mapping of phosphorus concentration in soybean seed. Euphytica, 2013, 189: 261–269 [15]Yan X, Liao H, Beebe S E, Blair M W, Lynch J P. QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean. Plant and Soil, 2004, 265: 17–29 [16]Yang M, Ding G D, Shi L, Xu F S, Meng J L. Detection of QTL for phosphorus efficiency at vegetative stage in Brassica napus. Plant & Soil, 2011, 339: 97–111 [17]Su J Y, Xiao Y M, Li M, Liu Q Y, Li B, Tong Y P, Jia J Z, Li Z S. Mapping QTLs for phosphorus-deficiency tolerance at wheat seedling stage. Plant & Soil, 2006, 281: 25–36 [18]Su J Y, Zheng Q, Li H W, Li B, Jing R L, Tong Y P. Detection of QTLs for phosphorus use efficiency in relation to agronomic performance of wheat grown under phosphorus sufficient and limited conditions. Plant Sci, 2009, 176: 824–836 [19]Guo Y, Kong F M, Xu Y F, Zhao Y, Liang X, Wang Y Y, An D, Li S. QTL mapping for seedling traits in wheat grown under varying concentrations of N, P and K nutrients. Theor Appl Genet, 2012, 124: 851–865 [20]Kjar B, Jensen J. The inheritance of nitrogen and phosphorus content in barley analysed by genetic markers. Hereditas, 1995, 123: 109–119 [21]Gong X, Wheeler R, Bovill W D, Mcdonald G K. QTL mapping of grain yield and phosphorus efficiency in barley in a Mediterranean-like environment. Theor Appl Genet, 2016, 129: 1657–1672 [22]唐旭, 陈义, 吴春艳, 杨生茂, 刘玉学, 吕豪豪, 马义兵, 李菊梅. 大麦长期肥料效率和土壤养分平衡. 作物学报, 2013, 39: 665–672 Tang X, Chen Y, Wu C Y, Yang S M, Liu Y X, Lyu H H, Ma Y B, Li J M. Fertilizer efficiency and soil apparent nutrient balance for barley under long-term fertilization. Acta Agron Sin, 2013, 39: 665–672 (in Chinese with English abstract) [23]鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000. pp 309–314 Lu R K. Analytical Methods of Soil and Agrochemistry. Beijing: China Agricultural Science and Technology Press, 2000. pp 309–314 (in Chinese) [24]Van O J W. JointMap 4, Software for the calculation of genetic linkage maps in experimental populations. Wageningen, 2006 (http://www.kyazma.nl/docs/JM4manual.pdf) [25]Van O J W. MapQTL version 6.0, Software for the mapping of quantitative trait loci in experimental populations of diploid species. Wageningen, 2009. https://www.kyazma.nl/docs/MQ6Manual.pdf [26]Chen A, Chen X, Wang H, Liao D H, Gu M, Qu H Y, Sun S B, Xu G H. Genome-wide investigation and expression analysis suggest diverse roles and genetic redundancy of Pht1 family genes in response to Pi deficiency in tomato. BMC Plant Biol, 2014, 14: 61 [27]Karandashov V, Bucher M. Symbiotic phosphate transport in arbuscular mycorrhizas. Trends Plant Sci, 2005, 10: 22–29 [28]Guo B, Jin Y, Wussler C, Blancaflor E B , Motes C M, Versaw W K. Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters. New Phytol, 2008, 177:889–898 [29]Li J Z, Xie Y, Dai A Y, Liu L F, Li Z C. Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice. J Genet Genomics, 2009, 36: 173–183 [30]崔世友, 耿雷跃, 孟庆长, 喻德跃. 大豆苗期耐低磷性及其QTL定位. 作物学报, 2007, 33: 378–383 Cui S Y, Geng L Y, Meng Q C, Yu D Y. QTL mapping of phosphorus deficiency tolerance in soybean (Glycine max L.) during seedling stage. Acta Agron Sin, 2007, 33: 378–383 (in Chinese with English abstract) [31]Zhang H W, Huang Y, Ye X S, Shi L, Xu F S. Genotypic differences in phosphorus acquisition and the rhizosphere properties of Brassica napus in response to low phosphorus stress. Plant & Soil, 2009, 320: 91–102 [32]Beebe S E, Rojaspierce M, Yan X L, Blair M W, Pedraza F, Munoz F M, Tohme J, Lynch J P. Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean. Crop Sci, 2006, 46: 413–423 [33]Devos K M. Updating the ‘Crop Circle’. Curr Opin Plant Biol, 2005, 8: 155–162 [34]李玉京, 刘建中, 李滨, 李继云, 姚树江, 李振声. 普通小麦基因组中耐低磷胁迫特性的染色体控制. 遗传学报, 1999, 26: 529–538 Li Y J, Liu J Z, Li B, Li J Y, Yao S J, Li Z S. Chromosomal control of the tolerance to phosphorus deficiency in genome of Triticum aestivum Chinese Spring. Acta Genet Sin, 1999, 26: 529–538 (in Chinese with English abstract) [35]Versaw W K, Harrison M J. A Chloroplast phosphate transporter, PHT2;1, influences allocation of phosphate within the plant and phosphate-starvation responses. Plant Cell, 2002, 14: 1751–1766 [36]Mudge S R, Rar A L, Diatloff E, Smith F W. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341–353 [37]Preuss C P, Huang C Y, Gilliham M, Tyerman S D. Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes. Plant Physiol, 2010, 152:1431–1441 [38]Chen J, Xu L, Cai Y. QTL mapping of phosphorus efficiency and relative biologic characteristics in maize (Zea mays L.) at two sites. Plant & Soil, 2008, 313: 251–266 [39]李利华, 邱旭华, 李香花, 王石平, 练兴明. 低磷胁迫水稻根部基因表达谱研究. 中国科学C辑: 生命科学, 2009, 39: 549–558 Li L H, Qiu X H, Li X H, Wang S P, Lian X M. The research of rice root’s gene expression profile on the condition of phosphorus deficiency stress. Sci Sin Vitae, 2009, 39: 549–558 (in Chinese) [40]Kuraparthy V, Sood S, Dhaliwal H S, Chhuneja P, Gill B S. Identification and mapping of a tiller inhibition gene (tin3) in wheat. Theor Appl Genet, 2007, 114: 285–294 [41]Luo Z W, Wu C I, Kearsky M J. Precision and high-resolution mapping of quantitative trait loci by use of recurrent selection, backcross or intercross schemes. Genetics, 2002, 161: 915–929 [42]Price A H. Believe it or not, QTLs are accurate. Trends Plant Sci, 2006, 11: 213–216 [43]The International Barley Genome Sequencing Consortium. A physical, genetic and functional sequence assembly of the barley genome. Nature, 2012, 491: 711–716 |
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