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作物学报 ›› 2015, Vol. 41 ›› Issue (08): 1147-1154.doi: 10.3724/SP.J.1006.2015.01147

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

基于EST-SSR和SNP标记的大麦麦芽纯度检测

张利莎1,2,董国清1,*,扎桑2,3,卓嘎3,王德良4,谷方红4,袁兴淼2,张京2,郭刚刚2,*   

  1. 1武汉轻工大学生物与制药工程学院，湖北武汉 430023；2农业部作物种质资源与生物技术重点开放实验室 / 国家农作物基因资源与基因改良重大科学工程 / 中国农业科学院作物科学研究所，北京 100081；3西藏大学农牧学院，西藏林芝 860000；4 中国食品发酵工业研究院，北京 100027
  • 收稿日期:2014-12-15 修回日期:2015-04-02 出版日期:2015-08-12 网络出版日期:2015-05-18
  • 通讯作者: 董国清, E-mail: dongbio@126.com; 郭刚刚, E-mail: guoganggang@caas.cn
  • 基金资助:

    本研究由“十二五”农村领域国家科技支撑计划项目(2013BAD01B05-2, 2012BAD03B01-2), 科技部国际合作项目(2013DFR30890-1)和国家现代农业产业技术体系建设专项(CARS-05)资助。

EST-SSR and SNP Markers Based Barley Malt Purity Detection

ZHANG Li-Sha1,2,DONG Guo-Qing1,*,ZHA Sang2,3,ZHUO Ga3,WANG De-Liang4,GU Fang-Hong4,YUAN Xing-Miao2,ZHANG Jing2,GUO Gang-Gang2,*   

  1. 1 School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2 Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, China; 4 China National Research Institute of Food and Fermentation Industries, Beijing 100027, China
  • Received:2014-12-15 Revised:2015-04-02 Published:2015-08-12 Published online:2015-05-18
  • Contact: 董国清, E-mail: dongbio@126.com; 郭刚刚, E-mail: guoganggang@caas.cn

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被引次数

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摘要：

大麦麦芽作为啤酒酿造的主要原料之一，其纯度决定了麦芽原料的均一性，进而影响加工工艺和啤酒品质。为高效准确地鉴定麦芽纯度，在啤酒企业进行麦芽原料采购和质量监测时提供参考依据。本研究分别利用EST-SSR和SNP标记定性检测了按比例预混的麦芽样品纯度，并利用SNP标记定量检测了4份送检的麦芽盲样纯度。结果表明，EST-SSR标记能定性检测混杂度高于10%的麦芽样品，而SNP标记能够有效鉴定混杂度低至5%的麦芽样品。SNP标记对纯度定量检测的单次抽样的测定值与真实值之间的误差在3%以内。比较发现，本研究所用的两类分子标记均可用于麦芽样品的纯度检测，但基于KASP技术的SNP标记可以满足麦芽纯度的快速定量检测需要。

关键词: 大麦麦芽, 纯度检测, 分子标记, KASP技术

Abstract:

Barley malt is one of the main raw materials used in beer production. Malt purity determines the level of its homogeneity, which affecting processing techniques and beer quality. For the efficient and accurate identification of malt purity, providing evidence for the malt raw materials procurement and quality monitoring in beer production, the purities of six proportionally premixed malt samples and another four blind samples were detected by using EST-SSR and SNP markers in this study. The results showed that the samples with mixing ratio higher than 10% were distinguished with EST-SSR markers in qualitative detection, whereas those with SNP markers were more effective even the sample impurity as low as 5%. In the quantitative detection, the standard error between the measured value and the true value was lower than 3% in one sampling. Obviously, both of the two types of molecular markers are all can be used for malt purity test, but KASP assay based-SNP detection is more suitable for rapid and quantitative malt purity test.

Key words: Barley malt, Purity test, Molecular marker, KASP

[1]国家统计局. 中国统计年鉴: 2013. 北京: 中国统计出版社, 2014



National Bureau of Statistics of the People’s Republic of China. China Statistical Yearbook: 2013. Beijing: China Statistics Press, 2014 (in Chinese)



[2]陈之贵. 啤酒厂加强麦芽质量控制的措施. 啤酒科技, 2002, (7): 24–25



Chen Z G. Malt quality control in brewery. Beer Sci Technol, 2002, (7): 24–25 (in Chinese)



[3]廖加宁. 麦芽对啤酒质量的影响及控制. 啤酒科技, 2006, (5): 31–33



Liao J N. Effect of malt on beer quality and the practice of quality control. Beer Sci Technol, 2006, (5): 31–33 (in Chinese)



[4]冯景章. 麦芽质量统计分析以及企业对大麦麦芽质量的要求与控制. 啤酒科技, 2006, (9): 17–19



Feng J Z. Analysis of malt quality and its requirement and control in beer enterprises. Beer Sci Technol, 2006, (9): 17–19 (in Chinese)



[5]颜启传, 黄亚军, 徐媛. 试用 ISTA 推荐的种子醇溶蛋白电泳方法鉴定大麦和小麦品种. 作物学报, 1992, 18: 61–68



Yan Q C, Huang Y J, Xu Y. Cultivar identification of barley and wheat with standard reference method from International Seed Test Association (ISTA). Acta Agron Sin, 1992, 18: 61–68 (in Chinese with English abstract)



[6]滕晓月, 陶龙兴, 孙雷心. 小麦品种的蛋白电泳鉴定. 作物学报, 1988, 14: 322–327



Teng X Y, Tao L X, Sun L X. Identification of wheat proteins by electrophoresis. Acta Agron Sin, 1988, 14: 322–328 (in Chinese with English abstract)



[7]佘花娣, 陈景堂, 黄亚群, 祝丽英, 池书敏. 利用DNA指纹图谱进行农作物品种鉴定的研究进展. 河北农业大学学报, 2003, 26(增刊): 28–30



She H D, Chen J T, Huang Y Q, Zhu L Y, Chi S M. Progress on variety identification of crop using DNA fingerprinting. J Agric Univ Hebei, 2003, 26(suppl): 28–30 (in Chinese with English abstract)



[8]谷方红, 张五九, 冯景章, 林智平．酿造大麦品种鉴定技术的研究. 啤酒科技, 2003, (11): 27–29



Gu F H, Zhang W J, Feng J Z, Lin Z P. Identification technologies of malt barley varieties. Beer Sci Technol, 2003, (11): 27–29 (in Chinese)



[9]Tinker N A, Fortin M G, Mather D E. Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet, 1993, 85: 976–984



[10]黄祥斌, 杨华林．采用PCR-RAPD技术鉴定16种北美啤酒大麦. 啤酒科技, 2003, (10): 52–54



Huang X B, Yang H L. Identification of 16 beer barley varieties using PCR-RAPD technique. Beer Sci Technol, 2003, (10): 52–54 (in Chinese)



[11]Russell J, Fuller J, Young G, Thomas B, Macaulay M, Waugh R, Powell W, Taramino G. Discriminating between barley genotypes using microsatellite markers. Genome, 1997, 40: 442–450



[12]兰青阔, 张桂华, 王永, 赵新, 朱珠, 崔兴华, 郭永泽, 程奕. 基于SNP标记的黄瓜杂交种纯度鉴定方法. 中国蔬菜, 2012, (6): 58–63



Lan Q K, Zhang G H, Wang Y, Zhao X, Zhu Z, Cui X H, Guo Y Z, Cheng Y. SNP-based molecular assay for cucumber hybrid seed purity identification by pyrosequencing. China Vegetables, 2012, (6): 58–63 (in Chinese with English abstract)



[13]Yu H H, Xie W B, Li J, Zhou F S, Zhang Q F. A whole-genome SNP array (RICE6K) for genomic breeding in rice. Plant Biotechnol J, 2014, 12: 28-37



[14]谷方红, 李海峰, 张五九, 林智平, 冯景章.采用微卫星技术进行大麦及麦芽的品种鉴定. 啤酒科技, 2005, (11): 26–28



Gu F H, Li H F, Zhang W J, Lin Z P, Feng J Z. Identification of barley and malt varieties using SSR markers. Beer Sci Technol, 2005, (11): 26–28 (in Chinese)



[15]Giancola S, McKhann H I, Bérard A, Camilleri C, Durand S, Libeau P, Roux F, Reboud X, Gut I G, Brunel D. Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants. Theor Appl Genet, 2006, 112: 1115–1124



[16]袁芳, 徐进, 季林丹, 费丽娟, 刘盼盼, 张莉娜. Tm-shift基因分型方法在遗传学中的应用. 遗传, 2012, 34: 1484–1490



Yuan F, Xu J, Ji L D, Fei L J, Liu P P, Zhang L N. Application of Tm-shift genotyping method in genetic studies. Hereditas (Beijing), 2012, 34: 1484–1490 (in Chinese with English abstract)



[17]Guo G G, Dondup D W, Zhang L S, Hu S, Yuan X M, Zhang J. Identification of SNPs in barley (Hordeum vulgare L.) by deep sequencing of six reduced representation libraries. Crop J, 2014, 2: 419–425



[18]Lister D L, Jones H, Jones M K, O'Sullivan D M, Cockram J. Analysis of DNA polymorphism in ancient barley herbarium material: validation of the KASP SNP genotyping platform. Taxon, 2013, 62: 779–789



[19]马蕾蕾, 徐世昌, 曹远银. 植物种子DNA快速提取新方法. 沈阳农业大学学报, 2005, 36: 626–627



Ma L L, Xu S C, Cao Y Y. A new protocol for fast DNA extraction from plant seeds. J Shenyang Agric Univ, 2005, 36: 626–627 (in Chinese with English abstract)



[20]Sharp P J, Kreis M, Shewry P R, Gale M D. Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75: 286–290
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