大麦原生质体培养再生胚性愈伤组织和白化苗

作物学报 ›› 1993, Vol. 19 ›› Issue (06): 520-522.

大麦原生质体培养再生胚性愈伤组织和白化苗

刘宝;吴琴生;刘大钧

南京农业大学细胞遗传研究室江苏南京

收稿日期:1992-03-24 修回日期:1992-08-19 出版日期:1993-11-12 网络出版日期:1993-11-12

Barley Protoplast Culture:Embryogenic Callus Formation and Albino Plantlet Regeneration

Liu Bao;Wu Qin-sheng;Liu Da-jun

Cytogenetics Lab., Nanjing Agricultural University,Nanjing 210014

Received:1992-03-24 Revised:1992-08-19 Published:1993-11-12 Published online:1993-11-12

摘要/Abstract

摘要： 从来自春大麦品种“如车”成熟胚的愈伤组织中,挑选出适于悬浮培养的松脆型胚性愈伤组织,在短期内建立胚性细胞悬浮系。此系酶解后分离出的原生质体在修改的MS培养基上能够持续分裂形成愈伤组织。将其直接转至分化培养基上获得结构紧密的胚性愈伤组织并再生白化苗.

关键词: 大麦（Hordeum vulgars L.）, 胚性细胞悬浮系, 原生质体培养, 白化苗

Abstract: Friable embryogenic callus was induced from seeds of spring barley cv. Ruche' cul-tured on MS medium with 3 mg/ L 2, 4--D, 0.2mg/L Kinetin, 200mg/L caseinhydrolysate, 300mg/L glutamine and 30g/L sucrose. These calli were used as sourcematerial for liquid culture and stable embryogenic cell suspensio

Key words: Hordeum vulgare L., Embryogenic cell suspension, Protoplast culture, Albino plantlet

刘宝;吴琴生;刘大钧. 大麦原生质体培养再生胚性愈伤组织和白化苗[J]. 作物学报, 1993, 19(06): 520-522.

Liu Bao;Wu Qin-sheng;Liu Da-jun. Barley Protoplast Culture:Embryogenic Callus Formation and Albino Plantlet Regeneration[J]. Acta Agron Sin, 1993, 19(06): 520-522.

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