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作物学报 ›› 2009, Vol. 35 ›› Issue (3): 530-534.doi: 10.3724/SP.J.1006.2009.00530

• 耕作栽培·生理生化 • 上一篇    下一篇

干旱胁迫下一氧化氮对小麦离体根尖离子吸收的影响

赵立群1;刘玉良1;孙宝腾2;王彩琴3   

  1. 1河北师范大学生命科学学院,河北石家庄050016;2南昌大学生命科学学院,江西南昌330031;3甘肃省人民医院药剂科,甘肃兰州730000
  • 收稿日期:2008-09-26 修回日期:2008-10-25 出版日期:2009-03-12 网络出版日期:2009-01-16
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2006CB100101);教育部留学回国人员科研启动基金(2008890)资助

Effect of Nitric Oxide on Ions Absorption in Excised Root Tips of wheat Seedlings under Drought Stress

ZHAO Li-Qun1;LIU YU-Liang1;SUN Bao-Teng2;WANG Cai-Qin3   

  1. 1School of Life Sciences, Hebei Normal University,Shijiazhuang 050016,China;2School of Life Sciences, Nanchang University, Nanchang 330031,China;3 Department of Pharmacy, People's Hospital of Gansu Province, Lanzhou 730000,China
  • Received:2008-09-26 Revised:2008-10-25 Published:2009-03-12 Published online:2009-01-16

PDF

1219

被引次数

9

摘要：

为阐明干旱胁迫下一氧化氮(NO)对植物的保护机制，利用干旱敏感性不同的3个小麦(Triticum aestivum L.)品种的离体根尖，比较了NO对干旱胁迫的响应及其对离子吸收的影响。在干旱胁迫下, 耐旱品种陇春8139根尖中大量产生NO, K+和Ca2+被大量吸收, 而Cl-1被排出体外, 质膜H+-ATPase活性升高; 而干旱敏感品种甘麦8和定西24的根尖中NO、离子含量和质膜H+-ATPase活性的变化呈相反趋势。NO供体硝普纳(SNP)处理使3个品种根尖中的K+和Ca2+含量增加，Cl-1含量下降，并能提高质膜H+-ATPase活力；NOS抑制剂Nω-nitro-L-arginine(LNNA)和NO清除剂2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl- 3-oxide(PTIO)能够逆转这一效果。Na+含量在所有处理下都没有明显变化。试验结果证明，NO能够通过调节质膜H+-ATPase活力影响植物对离子的选择吸收，从而提高耐旱性。

关键词: 干旱胁迫, 离子吸收, 一氧化氮, 质膜H+-ATPase

Abstract:

Longchun 8139 is a wheat (Triticum aestivum L.) cultivar grown in Dingsxi in Northwest China, which has stably tolerance characteristics to drought stress in morphology, physiology, and genetics. To explain the protection mechanism of nitric oxide to plants under drought stress, Longchun 8139 and two drought-sensitive cultivars Ganmai 8 and Dingxi 24 were used for testing the adaptation to drought stress. The excised root tips (2±0.02 cm) from the seedlings were treated with 20% polyethylene glycol (PEG)-6000 to simulate drought stress. In addition, the NO content in root tips was adjusted with 0.2 mmol L-1sodium nitroprusside (SNP, as NO donor), and 0.3 mmol L-1 Nω-nitro-L-arginine (LNNA, a NO synthase inhibitor), and 0.4 mmol L-1 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO, a specific NO scavenger) adding in culture medium. In the root tips of Longchun 8139, the activity of NO synthase and NO content increased remarkably, the chloride (Cl-) content decreased, whereas, the contents of calcium (Ca2+) and potassium (K+) increased, simultaneously, the activity of plasma membrane H+-ATPase was activated under drought stress. However, in the root tips of Ganmai 8 and Dingxi 24, the changing patterns of the parameters above were opposite. When applying SNP, changes of the contents of Ca2+ and K+, as well as the activity of plasma membrane H+-ATPase were observed similar to those induced by PEG in drought-tolerant cultivar, Longchun 8139. These changes in response to drought stress could be counteracted, such as, the promotion of Cl- content, the reductions of Ca2+ and K+ content, and the decrease of activity of plasma membrane H+-ATPase, if LNNA and PTIO were added in the culture medium. As to Na+ content, no obvious variations were observed among all treatments. The results indicate that NO acts as the second messenger in inducing drought resistance through influencing ions absorption, which is dependent on the increased activity of plasma membrane H+-ATPase.

Key words: Drought stress, Ion absorption, Nitric oxide, Plasma membrane H+-ATPase

[1] Chen S, Wang S, Altman A, Huttermann A. Genotypic variation in drought tolerance of poplar in relation to abscisic acid. Tree Physiol, 1997, 17: 797–803
[2] Anbar M. Nitric oxide: a synchronizing chemical messenger. Experientia, 1995, 51: 481–490
[3] Crawford N M, Guo F Q. New insights into nitric oxide metabolism and regulatory function. Trends Plant Sci, 2005, 10: 195–200
[4] Pedroso M C, Durzan D J. Effects of different gravity environments on DNA fragmentation and cell death in Kalanchoe leaves. Ann Bot, 2000, 86: 983–994
[5] Delledonne M, Xia Y J, Dixon R A, Lamb C. Nitric oxide functions as a signal in plant disease resistance. Nature, 1998, 394: 585–588
[6] Beligni M V, Lamattina L. Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissue. Planta, 1999, 208: 337–344
[7] Mata C G, Lamattina L. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiol, 2001, 126: 1196–1204
[8] Zhao L, Zhang F, Guo J, Yang Y, Li B, Zhang L. Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed. Plant Physiol, 2004, 134:849－857
[9] Li H, Zhang D Y. Morphological characteristics and growth redundancy of spring wheat root system in semi-arid regions. J Appl Ecol, 1999, 10: 26–30
[10] White P R. The Cultivation of Animal and Plant Cells. New York: Ronald Press, 1963. pp 57–77
[11] Murphy M E, Noack E. Nitric oxide assay using hemoglobin method. Methods Enzymol, 1994, 233: 240–250
[12] Song L, Ding W, Zhao M, Sun B, Zhang L. Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed. Plant Sci, 2006, 171: 449–458
[13] Sairam R K, Srivastava G C. Changes in antioxidant activity in subcellular fraction of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Sci, 2002, 162: 897–904
[14] Chen S, Li J, Fritz E, Wang S, Huttermann A. Sodium and chloride distribution in roots and transport in three poplar genotypes and under increasing salt stress. For Ecol Manag, 2002, 168: 217–230
[15] Qiu Q S, Su X F. The influence of extracellular-side Ca2+ on the activity of the plasma membrane H+-ATPase from wheat roots. Aust J Plant Physiol, 1998, 25: 923–928
[16] Zhang L, Deng X. Advances in studies on physiology and biochemistry of wheat drought resistance. Agric Res Arid Areas, 2000, 18: 87–92
[17] Zhu J K. Plant and salt tolerance. Trends Plant Sci, 2001, 6: 66–71
[18] Qiu Q-S (邱全胜). Influence of K+ on the coupling between ATP hydrolysis and proton transport by the plasma membrane H+-ATPase from soybean hypocotyls. Acta Bot Sin (植物学报), 1999, 41(9): 962–966 (in Chinese with English abstract)
[19] Gupta A S, Berkowitz G A, Pier P A. Maintenance of photosynthesis at low leaf water potential in wheat. Plant Physiol, 1989, 89: 1358–1365
[20] Zimmermann S, Ehrhardt T, Plesch G, Muller-Rober B. Ion channels in plant signaling. Cell Mol Life Sci, 1999, 55: 183–203
[21] Monroy A F, Sangwan V, Dhindsa R S. Low temperature signal transduction during cold acclimation: protein phosphates 2A as an early target for cold-inactivation. Plant J, 1998, 13: 653–660
[22] Michelet B, Boutry M. The plasma membrane H+-ATPase: a highly regulated enzyme with multiple physiological functions. Plant Physiol, 1995, 108: 1–6
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