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作物学报 ›› 2009, Vol. 35 ›› Issue (4): 749-754.doi: 10.3724/SP.J.1006.2009.00749

• 研究简报 • 上一篇    下一篇

皖南不同类型土壤植烟成熟期烟叶的基因差异表达和显微结构的比较

邱立友1，李富欣2，宜朝龙3，马称心3，杨超2，冯云1，刘国顺2，赵会杰1*   

  1. 1河南农业大学生命科学学院，河南郑州450002；2河南农业大学农学院，河南郑州450002；3安徽皖南烟叶有限公司，安徽宣城242000
  • 收稿日期:2008-06-04 修回日期:2008-12-15 出版日期:2009-04-12 网络出版日期:2009-02-16
  • 通讯作者: 赵会杰，0371-63555319; E-mail: zhaohj303@163.com
  • 基金资助:

    本研究由国家烟草专卖局重大科技攻关项目（20060202）资助。

Comparison on Gene Expression and Microstructure of Tobacco Leaves at Maturity Stage in Different Types of Soil in southern Anhui Province,China

QIU Li-You1,LI Fu-Xin2,ZU Chao-Long3,MA Chen-Xin3,YANG Chao2,FENG Yun1,LIU Guo-Shun2,ZHAO Hui-Jie1*   

  1. 1College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China;2Agronomy College,Henan Agricultural University,Zhengzhou 450002,China;3Anhui Wannan Tobacco Leaf Co.Ltd.,Xuancheng 242000,China
  • Received:2008-06-04 Revised:2008-12-15 Published:2009-04-12 Published online:2009-02-16
  • Contact: ZHAO Hui-Jie

PDF

1564

被引次数

40

摘要：

以烟草品种云烟97为材料，在皖南烟区典型的植烟土壤麻沙土、麻沙泥、粉沙土种植，以水稻土种植作对照，应用基因芯片技术和显微观察技术对生长成熟期烟叶的基因表达谱和细胞结构进行比较。结果表明，与水稻土相比，麻沙土植烟成熟期烟叶上调表达的基因主要有细胞增殖、生长和分化的基因，以及与氧化胁迫有关的基因，而下调表达的基因主要是光合作用和蛋白质、磷脂合成的基因。麻沙泥植烟成熟期烟叶上调表达的基因主要有与生长素运输和多糖合成有关的基因及与蛋白质和氨基酸分解有关的基因。粉沙土植烟成熟期烟叶与干旱胁迫有关的基因和与纤维素、果胶质、淀粉和蛋白质分解有关的基因上调。叶片的显微和超显微结构表明，粉沙土和水稻土植烟，烟叶长势差，生长衰退迹象明显；而麻沙土和麻泥土植烟，尤其是麻沙土，烟叶生长势较好，生长衰退迹象不明显。

关键词: 烟草, 土壤类型, 成熟期, 基因芯片, 显微结构

Abstract:

Yunyan 97(Nicotiana tabacum) was planted in four types of soil including coarse sandy soil, coarse sandy mud, powder sandy soil, and rice soil (CK), in the south of Anhui. The gene expression profile and cell construction of tobacco leaves at maturity stage were explored using cDNA microarray and microscopical technique. Comparing tobacco leaves grown in coarse sandy soil to rice soil, up-regulated genes were mainly for cell multiplication, growth, development, and oxidation stress, while genes in photosynthesis, protein and phospholipids synthesis were down-regulated. Specifically up-regulated genes involved in auxin transport, polysaccharide synthesis, and responsible for protein and amino acid degradation in tobacco leaves cultivated in coarse sandy mud briefly compared with rice soil. Compared to rice soil, up-regulated genes of tobacco leaves in powder sandy soil principally dealt with drought stressand degrading cellulose, pectin, starch, and protein. The microscopic and ultramicroscopic structure of the tobacco leaves revealed that tobacco leaves in powder sandy soil and rice soil grew badly. On the contrary, coarse sandy mud and especially coarse sandysoil produced well grown leaves. The results should conduce to unveil the mechanism of burnt-sweet sensation formation in tobacco leaves cultivated in the south of Anhui Province.

Key words: Tobacco, Soil type, Maturity stage, cDNA microarray, Microstructure

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