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作物学报 ›› 2017, Vol. 43 ›› Issue (07): 993-1002.doi: 10.3724/SP.J.1006.2017.00993

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

耐低氮烟草基因型的筛选及氮效率分析

钟思荣1,陈仁霄2,陶瑶1,龚丝雨1,何宽信2,张启明2,张世川1,刘齐元1,*   

  1. 1江西农业大学农学院 / 作物生理生态与遗传育种教育部重点实验室 / 江西省作物生理生态与遗传育种重点实验室,江西南昌330045;2江西省烟叶科学研究所,江西南昌330045
  • 收稿日期:2016-11-09 修回日期:2017-01-21 出版日期:2017-07-12 网络出版日期:2017-02-17
  • 通讯作者: 刘齐元, E-mail: qiyuanl@126.com
  • 基金资助:

    本研究由江西省烟草专卖局科技项目(201401001)资助。

Screening of Tobacco Genotypes with Tolerance to Low-Nitrogen and Analysis of Their Nitrogen Efficiency

ZHONG Si-Rong1,CHEN Ren-Xiao2,TAO Yao1,GONG Si-Yu1,HE Kuan-Xin2,ZHANG Qi-Ming2,ZHANG Shi-Chuan1,LIU Qi-Yuan1,*   

  1. 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; 2 Jiangxi Leaf Tobacco Research Institute, Nanchang 330045, China
  • Received:2016-11-09 Revised:2017-01-21 Published:2017-07-12 Published online:2017-02-17
  • Contact: 刘齐元, E-mail: qiyuanl@126.com
  • Supported by:

    This study was supported by the Jiangxi Province Tobacco Monopoly Bureau of Science and Technology Projects (201401001).

摘要:

筛选耐低氮及氮高效的烟草基因型是提高氮素利用效率,减少氮污染的一种有效途径。本文采用营养液培养方法,于苗期以低氮(0.5 mmol L–1)和正常氮(5.0 mmol L–1)处理74个不同基因型烟草,通过指标统计、因子分析、聚类分析来确定评价指标及筛选出耐低氮基因型,并结合氮效率综合值分析表明,在低氮和正常氮条件下,不同基因型烟草的根系体积、根系生物量、茎叶氮累积量、地上部生物量变异系数均较大,分别为0.37~0.68和0.38~0.64。低氮和正常氮的主成分基本相似,在不同供氮条件下茎叶氮累积量、地上部生物量均起主要作用。筛选出15个耐低氮基因型,占供试材料的20.3%,其中8个属于低氮高效正常氮低效型,占耐低氮基因型的53.3%,6个属于低氮低效正常氮低效型,占40.0%,1个属于低氮高效正常氮高效型,占0.7%;筛选出8个低氮敏感基因型,其中6个属于低氮低效正常氮高效型,占75.0%,2个属于低氮低效正常氮低效型,占25.0%。初步确定14P9为耐低氮、氮高效基因型,中烟100和K394为低氮敏感、氮低效基因型。

关键词: 烟草, 基因型, 苗期, 氮素, 氮高效

Abstract:

It is an effective way to screen tobacco genotypes with tolerance to low-nitrogen which increase nitrogen use efficiency and decreased nitrogen pollution. In this paper, 74 tobacco genotypes were treated with low nitrogen (0.5 mmol L–1) and normal nitrogen (5.0 mmol L–1) levels in hydroponics at seedlings stage, and evaluated and screened by using descriptive statistics, factor analysis and cluster analysis The variation coefficient of root volume, root biomass, stalk and leaf nitrogen accumulation, aboveground biomass were relatively large under low nitrogen and normal nitrogen conditions, with rang of 0.37–0.68 and 0.38–0.64, respectively. The principal components were similar for both treatments; the stalk and leaf nitrogen accumulation and aboveground biomass played a major role. According to heatmap cluster analysis and scatter diagram analysis, 15 genotypes with tolerance to low-nitrogen were screened out, accounting for 20.3% of the test materials, eight of which were low nitrogen high efficiency and normal nitrogen low efficiency genotypes, accounting for 53.3% of the low-nitrogen tolerant genotypes, six were low nitrogen low efficiency and normal nitrogen low efficiency genotypes, accounting for 40.0%, and one was low nitrogen high efficiency and normal nitrogen high efficiency, accounting for 0.7%. Eight low-nitrogen sensitive genotypes were screened out, among them six belonged to low nitrogen low efficiency and normal nitrogen high efficiency, accounting for 75.0%, two to low nitrogen low efficiency and normal nitrogen low efficiency, accounting for 25.0%. The study suggested that 14P9 was low-nitrogen tolerance and nitrogen high efficiency genotype, while Zhongyan 100 and K394 were low-nitrogen sensitive and nitrogen low efficiency genotypes.

Key words: Tobacco, Genotype, Seedling stage, Nitrogen, High nitrogen efficiency

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