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作物学报 ›› 2011, Vol. 37 ›› Issue (12): 2277-2284.doi: 10.3724/SP.J.1006.2011.02277

• 研究简报 • 上一篇    下一篇

SRAP和ISSR及两种方法结合在分析黄麻属起源与演化上的比较

陶爱芬1,祁建民1,*,粟建光2,李爱青3,李木兰1,方平平1,林荔辉1,徐建堂1,吴建梅1,林培清1   

  1. 1 作物遗传育种与综合利用教育部重点实验室 / 福建农林大学，福建福州350002；2 中国农业科学院麻类研究所，湖南长沙410205；3 安徽省种子管理总站，安徽合肥230001
  • 收稿日期:2011-04-02 修回日期:2011-09-17 出版日期:2011-12-12 网络出版日期:2011-09-30
  • 通讯作者: 祁建民, E-mail: qijm863@163.com, Tel:0591-87644898
  • 基金资助:

    本研究由国家自然科学基金项目(31000734), 国家麻类产业技术体系(CARS-19-E05)和南方麻类种质资源平台建设项目(2010N2002)资助。

Method Comparison of Using SRAP and ISSR and Combination of Both in Origin and Evolution of Jute

TAO Ai-Fen1,QI Jian-Min1, SU Jian-Guang2,LI Ai-Qing3,LI Mu-Lan1,FANG Ping-Ping1,LIN Li-Hui1,XU Jian-Tang1,WU Jian-Mei1,LIN Pei-Qing1   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Bast Fiber Crops of Chinese Academy of Agriculture Sciences, Changsha 410205, China; 3 Anhui Seed Administration Station, Hefei 230001, China
  • Received:2011-04-02 Revised:2011-09-17 Published:2011-12-12 Published online:2011-09-30
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel:0591-87644898

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1166

被引次数

19

摘要： 选用96份黄麻属种质资源，比较了SRAP、ISSR及二者结合的方法在黄麻属起源与演化研究上的可行性。结果表明: (1)SRAP方法的多态性条带比率为100%，高于ISSR方法的98.1%，平均每条引物扩增出的多态性条带亦高于ISSR方法。(2)SRAP方法构建的进化树可大致将各类型种质资源区别开来，可较明确、清晰地展现黄麻属的起源与演化趋势。但无法区分开个别种质; ISSR方法构建的进化树将很多圆果黄麻品种聚在一起，无法区别开来，且分枝长度短，无法确定进化时间;(3)SRAP与ISSR结合构建的进化树将不同类型的黄麻种质资源有序排列，可清晰地明确其进化趋势及演化关系。比较而言，SRAP与ISSR分子标记结合的方法优于SRAP方法，而SRAP方法又优于ISSR方法，在进行相关研究时，应优先考虑采用SRAP与ISSR分子标记结合的方法。

关键词: 黄麻, 起源, 演化, SRAP, ISSR

Abstract: The objective of this study was to compare the feasibility of using SRAP, ISSR and combination of the both in origin and evolution of jute (Corchorus L.). Ninety-six jute genotypes were tested by these three methods. The results were as follows: (1) The percentage of polymorphic bands of SRAP was 100%, which was higher than that of 98.1% of ISSR, and the polymorphic bands amplified per primer by SRAP were also higher than these of ISSR. (2) On the dendrogram based on SRAP data, jute genotypes were close to be classified. The trend of origin and evolution of jute could be clarified. However, a few genotypes were misled to other taxonomic groups, which affected the accuracy of analysis on origin and evolution of jute. On the dendrogram based on ISSR data, many capsularis cultivars were found to be clustered together and couldn’t be identified clearly, and also the time of origin was difficult to confirm. (3) Based on the dendrogram derived from SRAP and ISSR data, different taxonomic groups of jute were found to be clustered orderly, and the relationship of origin and the trend of evolution within jute genus manifested clearly. (4) The strategy of SRAP and ISSR combination was better than that of SRAP alone, and then followed by ISSR. Hence, the strategy of SRAP and ISSR combination should have priority in studies on the origin and evolution of jute.

Key words: Jute, Origin, Evolution, SRAP, ISSR

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