作物学报 ›› 2014, Vol. 40 ›› Issue (07): 1213-1219.doi: 10.3724/SP.J.1006.2014.01213
张立武**,袁民航**,何雄威,刘星,方平平,林荔辉,陶爱芬,徐建堂,祁建民*
ZHANG Li-Wu**,YUAN Min-Hang**,HE Xiong-Wei,LIU Xing,FANG Ping-Ping,LIN Li-Hui,TAO Ai-Fen,XU Jian-Tang,QI Jian-Min*
摘要:
本研究从GenBank公共数据库中下载黄麻表达序列标签838条,利用SSRPrimer软件对其进行SSR位点查找,利用Primer 3.0软件设计66对SSR引物,通过琼脂糖凝胶研究这些SSR引物的PCR扩增特点,以检测其多态性。结果表明,66对SSR引物在黄麻属6个不同类型材料的扩增中,42 (63.6%)对引物至少在2个材料之间存在多态性。(AT)n重复基元和(GC-)n丰富的三核苷酸重复基元多态性较高,可作为黄麻SSR标记引物设计的首选。黄麻EST-SSR标记开发效率较高,不仅可以丰富黄麻分子标记的数量,而且为剖析黄麻重要性状的遗传机制奠定基础,这对于黄麻的遗传基础研究具有重要应用价值。
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