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作物学报 ›› 2014, Vol. 40 ›› Issue (07): 1205-1212.doi: 10.3724/SP.J.1006.2014.01205
刘长英1,吕蕊花1,朱攀攀1,范伟1,李军1,王晓红1,李镇刚2,王茜龄1,赵爱春1,鲁成1,余茂德1,*
LIU Chang-Ying1,LÜ Rui-Hua1,ZHU Pan-Pan1,FAN Wei1,LI Jun1,WANG Xiao-Hong1,LI Zhen-Gang2,WANG Xi-Ling1,ZHAO Ai-Chun1,LU Cheng1,YU Mao-De1,*
摘要：
EIN2是植物体内乙烯信号转导的中心元件，负责将乙烯信号由内质网传递到细胞核中。本文通过检索桑树基因组数据，获得一个EIN2候选基因(MaEIN2)，并进行生物信息学分析和表达分析。该MaEIN2全长5614 bp，由7个外显子和6个内含子组成，包含3921 bp的CDS，编码1036个氨基酸残基。MaEIN2在进化树中与草莓、桃树等双子叶植物的EIN2蛋白关系较近，与单子叶植物关系较远。MaEIN2在老叶和成熟果实中的表达量分别高于在幼叶和幼果中，且随果实发育呈逐渐上升趋势，MaEIN2可能与器官的成熟衰老有关。选用乙烯利、ABA和NaCl处理桑树种苗，乙烯利能够促进MaEIN2的表达，而ABA和NaCl抑制MaEIN2的表达。本文为深入研究MaEIN2基因的功能奠定了基础。
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