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作物学报 ›› 2014, Vol. 40 ›› Issue (07): 1220-1226.doi: 10.3724/SP.J.1006.2014.01220

• 耕作栽培·生理生化 • 上一篇    下一篇

缩节胺浸种提高棉花幼苗根系活力中的活性氧代谢

王宁1,2,田晓莉2,*,段留生2,严根土1,黄群1,李召虎2   

  1. 1 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室, 河南安阳 455000; 2 中国农业大学作物化学控制研究中心 / 植物生长调节剂教育部工程研究中心, 北京100193
  • 收稿日期:2013-11-23 修回日期:2014-04-16 出版日期:2014-07-12 网络出版日期:2014-05-16
  • 通讯作者: 田晓莉, E-mail: tianxl@cau.edu.cn, Tel: 010-62732039
  • 基金资助:

    本研究由国家自然科学基金项目(31271629)，国家现代农业产业技术体系建设专项(CCRS-18-18)和中央级公益性科研院所基本科研业务费专项(1610162014002)资金资助。

Metabolism of Reactive Oxygen Species Involved in Increasing Root Vigour of Cotton Seedlings by Soaking Seeds with Mepiquat Chloride

WANG Ning1,2,TIAN Xiao-Li2,*,DUAN Liu-Sheng2,YAN Gen-Tu1,HUANG Qun1,LI Zhao-Hu2   

  1. 1 State Key Laboratory of Cotton Biology / Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China; 2 Engineering Research Center of Plant Growth Regulator, Ministry of Education / Center of Crop Chemical Control, China Agricultural University, Beijing 100193, China
  • Received:2013-11-23 Revised:2014-04-16 Published:2014-07-12 Published online:2014-05-16
  • Contact: 田晓莉, E-mail: tianxl@cau.edu.cn, Tel: 010-62732039

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摘要：

以国欣棉3号为材料，研究200 mg L–1缩节胺(DPC)浸种12 h对棉花子叶苗根系活力的影响，并从活性氧(ROS)代谢的角度揭示相关的生理机制。结果表明，DPC浸种显著增强了棉花幼苗的根系活力，根尖部位氯化三苯基四氮唑(TTC)染色光密度为清水对照的1.3倍，TTC法测定的根系活力和呼吸速率分别较对照增加167%和90%，非损伤微测技术(NMT)测定的K+净内流速率 (距根尖300 μm处)较对照提高36%。吖啶橙染色结果显示，DPC处理根尖伸长区的凋亡细胞数目较对照减少。此外，DPC处理使根系的过氧化氢酶(CAT)、抗坏血酸氧化酶(APX)和谷胱甘肽还原酶(GR)活力显著高于对照，超氧化物歧化酶(SOD)活力则降低；H2O2含量和超氧阴离子(O2−)产生速率较对照分别降低56%和65%，H2O2原位染色结果也显示其根尖部分的褐色较对照明显减弱。根系组织的ROS代谢得到改善可能是DPC浸种提高棉花幼苗根系活力的机制之一。

关键词: 棉花, 缩节胺, 根系活力, 活性氧代谢

Abstract:

The plant growth regulator, mepiquat chloride (1,1-dimethylpiperidinium chloride, DPC) has been used worldwide to suppress excessive growth in cotton plants. It also increases the root vigour of cotton plants. To reveal the possible role of reactive oxygen species (ROS) involved, we conducted a experiment to investigate the effect of soaking seed with 200 mg L–1 mepiquat chloride (DPC) on root vigour of cotton (Gossypium hirsutum L.) seedlings by using a cotton cv. Guoxin 3. The results showed that soaking seed with DPC could significantly increase the root vigour of cotton seedlings with two expanded cotyledons.Root viability estimated by 2,3,5-triphenyl tetrazolium chloride (TTC) reduction and root respiratory rate were increased by 167% and 90% in DPC treatment. The average OD of in situ TTC staining in DPC treated root tips was 0.3-fold higher than that of control. At 300 μm from the root apex away, DPC treatment resulted in a 36% increase of net K+ influx. The result of acridine orange staining suggested that seedlings treated with DPC had less apoptotic cells in root elongation zone compared with control. In addition, DPC treatment significantly altered the activities of antioxidant enzyme and ROS accumulation in roots of cotton seedlings. Catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were enhanced, but superoxide dismutase (SOD) was reduced, which therefore resulted in 56% less H2O2 accumulation and lighter diaminobenzidine staining (H2O2 histochemical detection in situ) in roots compared with control. Another type of ROS, O2−, its production rate also decreased by 65% in roots treated with DPC. Taken together, we speculated that the increased root vigour of cotton seedlings by soaking seed with DPC may be partially explained by improvement of ROS metabolism.

Key words: Cotton (Gossypium hirsutum L), Mepiquat chloride, Root vigour, Reactive oxygen species (ROS) metabolism

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