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作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1833-1838.doi: 10.3724/SP.J.1006.2012.01833

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗根腐病的转GmPGIP3基因小麦扬麦18的获得与鉴定

党良1,2,**，王爱云1,3,**，徐惠君1，祝秀亮1，杜丽璞1，邵艳军2，张增艳1,*   

  1. 1中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081; 2河北农业大学生命科学学院, 河北保定071001;
    3中南林业科技大学生命科学与技术学院, 湖南长沙 410004
  • 收稿日期:2012-04-19 修回日期:2012-06-10 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 张增艳, E-mail: zhangzy@mail.caas.net.cn
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08002-001)资助。

Development and Characterization of GmPGIP3 Transgenic Wheat Yangmai 18 with Enhanced Resistance to Common Root Rot

DANG Liang1,2,**,WANG Ai-Yun1,3,**,XU Hui-Jun1,ZHU Xiu-Liang1,DU Li-Pu1,SHAO Yan-Jun2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Biology Science, Agricultural University of Hebei, Baoding 071001, China; 3 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
  • Received:2012-04-19 Revised:2012-06-10 Published:2012-10-12 Published online:2012-07-27
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn

PDF

963

被引次数

10

摘要：

GmPGIP3是大豆的一种多聚半乳糖醛酸酶抑制蛋白, 能够特异性地抑制部分病原真菌内切多聚半乳糖醛酸活性, 从而减弱病原菌对植株的侵害。利用基因重组技术构建了GmPGIP3基因的单子叶植物表达载体pA25-GmPGIP3, 通过基因枪介导法将pA25-GmPGIP3转入小麦品种扬麦18中。对转GmPGIP3基因扬麦18的T0至T2代植株进行PCR、Southern杂交、半定量RT-PCR和荧光定量Q-RT-PCR分析, 并对根腐病进行抗性鉴定。结果表明, GmPGIP3已转入扬麦18, 并在转基因小麦中遗传、转录和表达；比受体材料相比, 5个GmPGIP3过表达的转基因小麦株系对根腐病的抗性有明显提高。

关键词: 多聚半乳糖醛酸抑制蛋白, GmPGIP3, 转基因小麦, 分子检测, 小麦根腐病, 抗性

Abstract:

GmPGIP3 is a polygalacturonase-inhibiting protein from soybean, which could reduce the infection of fungal pathogen throughr inhibiting the endo-polygalacturonase activity of pathogen fungi. Using genetic recombination technique, we constructed the transformation vector of GmPGIP3 gene expressing highly in monocot plants, pA25-GmPGIP3, in which GmPGIP3 gene was driven by maize ubiquitin promoter. Embryo callus of Yangmai 18 was bombarded by the particle containing pA25-GmPGIP3 vector DNA. The GmPGIP3 transgenic wheat plants from T0 to T2 generations were detected by PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. The alien GmPGIP3 proved to be introduced into seven transgenic wheat lines with heritability and expression events. We also evaluated the disease resistance in these GmPGIP3 transgenic plants through inoculating the common root rot pathogen, Bipolaris sorokiniana. Compared with untransformated Yangmai 18, five GmPGIP3 transgenic lines showed significantly-enhanced resistance to Bipolaris sorokiniana.

Key words: Polygalacturonase-inhibiting protein (PGIP), GmPGIP3, Transgenic wheat, Molecular detection, Common root rot, Resistance

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