作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1827-1832.doi: 10.3724/SP.J.1006.2012.01827
张云龙1,2,王美蛟1,张悦1,褚翠萍2,林志珊1,*,徐琼芳1,叶兴国1,陈孝1,张宪省2
ZHANG Yun-Long1,2, WANG Mei-Jiao1, ZHANF Yue1, CHU Cui-Ping2, LIN Zhi-Shan1,*, XU Qiong-Fang1, YE Xing-Guo1, CHEN Xiao1,ZHANG Xian-Sheng2
摘要:
小麦6VS·6DL易位系Pm97033和6VS·6AL易位系92R137中的6VS染色体臂来自不同的簇毛麦种质,均表现良好的白粉病抗性,本研究利用分子标记对这2个易位系所包含抗病基因的异同进行了鉴定。利用与Pm21抗白粉病相关的丝氨酸/苏氨酸蛋白激酶Stpk-V基因(GenBank登录号为HQ864471.1)的基因组和cDNA序列为基础,在包含至少1个内含子的2个编码区设计引物,从Pm97033中扩增获得特异的多态性片段。为进一步提高特异性和扩增的稳定性,对特异扩增片段测序并重新设计引物,扩增筛选获得2个引物对,其中PK-F1/PK-R可专一扩增6VS·6DL易位系Pm97033及其抗病亲本,而PK-F2/PK-R可同时特异扩增2个不同来源的簇毛麦6VS染色体,但二者间的特异片段具有多态性。利用这2对引物,对系谱中包含6V(6D)和6VS·6AL、抗白粉病的小麦品系CB037进行检测,发现仅出现与6VS·6AL易位系相同的簇毛麦扩增片段,不存在簇毛麦No. 1026 (Pm97033的6VS供体)的扩增片段。基因组原位杂交结果表明,CB037仅含1对小麦-簇毛麦的易位染色体,用已报道的分子标记检测证明易位涉及的小麦染色体为6A,与本研究开发的分子标记检测结果相吻合,表明CB037携带的白粉病抗性基因来自6VS·6AL易位系92R137,其白粉病抗性可能与Pm97033具有不同的遗传基础。
[1]Bennett F G A. Resistance to powdery mildew in wheat: A review of its use in agriculture and breeding programmes. Plant Pathol, 1984, 33: 279–300[2]Agnieszka G D. The genus Dasypyrum-part 2. Dasypyrum villosum-a wild species used in wheat improvement. Euphytica, 2006, 152: 441–454[3]Blanco A, Simeone R, Resta P. The addition of Dasypyrum villosum (L.) Candargy chromosomes to durum wheat (Triticum durum Desf.). Theor Appl Genet, 1987, 74: 328–333[4]De Pace C, Montebove L, Delre V, Jan C C, Qualset C O, Scarascia Mugnozza G T. Biochemical versality of amphiploids derived from crossing Dasypyrum villosum Candargy and wheat: genetic control and phenotypical aspects. Theor Appl Genet, 1988 76: 513–529[5]Della Gatta C, Tanzarella O A, Resta P, Blanco A. Protein content in a population of Haynaldia villosa and electrophoretic pattern of the amphidiploid T. durum–H. villosa. In: Porceddu E eds Breeding methodologies in durum wheat and triticale. Univ of Tuscia, Viterbo, Italy, 1984. pp 39–43[6]Kong F-J(孔凡晶), Chen X(陈孝). Advance of study on D. villosum (H. villosa) genome and its application in wheat improvement. J Triticeae Crops (麦类作物学报), 2001, 21(2): 85–87 (in Chinese with English abstract)[7]Chen X(陈孝), Shi A-N(施爱农), Shang L-M(尚立民). The resisitance reaction of H. villosa to powdery mildery isolates and its expression in wheat background. Acta Phytopathol Sin (植物病理学报),1997,27(1) : 17–22 (in Chinese with English abstract) [8]Liu D-J(刘大钧), Chen P-D(陈佩度), Pei G-Z(裴广铮), Wang Y-N(王耀南). Studies on transfer of genetic material from Haynaldia villosa to Triticum aestivum. Acta Genet Sin (遗传学报), 1983, 10(2): 103–113 (in Chinese with English abstract)[9]Chen P-D(陈佩度), Zhou B(周波), Qi L-L(齐莉莉), Liu D-J(刘大钧). Identification of wheat–Haynaldia villossa amphiploid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a probe. Acta Genet Sin (遗传学报), 1995, 22(5): 380–386 (in Chinese with English abstract)[10]Li H(李辉), Chen X(陈孝), Xi Z-Y(辛志勇), Ma Y-Z(马有志), Xu H-J(徐惠君). Development and identification of wheat–Haynaldia villosa 6DL/6VS translocation lines with powdery mildew resistance. Sci Agric Sin (中国农业科学), 1999, 32(5): 9–15 (in Chinese with English abstract)[11]Liu P(刘萍), Yang B-J(杨宝军), Chen P-D(陈佩度). Transfer of disease resistance of Triticum aestivum–Haynaldia villosa 6VS/6AL translocation lines in different wheat background. J Nanjing Agric Univ (南京农业大学学报), 2004, 27(2): 1–5 (in Chinese with English abstract)[12]Chen X(陈孝), Xu H-J(徐慧君), Du L-P(杜丽璞), Shang L-M(尚立民), Han B(韩滨), Shi A-N(施爱农), Xiao S-H(肖世和). Transfer of gene resistance to powdery mildew from H. villosa to common wheat by tissue culture. Sci Agric Sin (中国农业科学), 1996, 29(5):1–8 (in Chinese with English abstract)[13]Shang L-M(尚立民), Chen X(陈孝), Xiao S-H(肖世和), Xu H-J(徐慧君), Shi A-N(施爱农). Genetic and biochemical identi?cation of common wheat–Haynaldia villosa new germplasms. Acta Agron Sin (作物学报), 1997, 23(2): 159–164 [14]Li H, Chen X, Xin Z Y, Xu H J, Du L P, Ma Y Z. Establishment of 6VS telocentric lines of Haynaldia villosa resistant to powdery mildew induced by immature embryo culture. Acta Bot Sin, 2002, 44(2): 127–131[15]Cao A Z, Xing L P, Wang X Y, Yang X M, Wang W, Sun Y L, Qian C, Ni J L, Chen Y P, Liu D J, Wang X E, Chen P D. Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA, 2011, 108: 7727–7732[16]Li H, Chen X, Xin Z Y, Ma Y Z, Xu H J, Chen X Y, Jia X. Development and identification of wheat–Haynaldia villosa T6DL•6VS chromosome translocation lines conferring resistance to powdery mildew. Plant Breed, 2005, 124: 203–205[17]Zhang Q-Q(张庆勤), Zhang L-Y(张立异), Zhu W-H(朱文化), Xie S-X(谢水仙). Utilization of Haynaldia villosa (L.) Shur in resistance breeding in wheat. Acta Phytophyl Sin (植物保护学报), 1998, 25(1): 1–5 (in Chinese with English abstract)[18]Porebski S, Bailey L G, and Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Report, 1997, 15: 8–15[19]Lalitha S. Primer premier 5. Biotechnol Softw Internet Rep, 2000, 1: 270–272[20]Wei W H, Qin R, Song Y C, Ning S B, Guo L Q, Gu M G. Location and analysis of introgressed segments in the parthenogenetic progenies of Zea mays × Z. diploperennis by GISH. Acta Bot Sin, 2002, 44: 373–376[21]Zhang Y(张悦), Lin Z-S(林志珊), Cao B-J(曹保久), Guo Y-Q(郭义强), Wang M-J(王美蛟), Ye X-G(叶兴国), Xin Z-Y(辛志勇), Xu Q-F(徐琼芳), Guo S-H(郭世华). Genetic behaviour of Thinopyrum intermedium chromosome 2Ai-2 in different wheat chromosome substitution backgrounds of group 2. Acta Agron Sin (作物学报), 2009, 35(3): 424−431 (in Chinese with English abstract)[22]Cao A Z, Wang X E, Chen Y P, Zou X W, Chen P D. A sequence-speci?c PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa. Plant Breed, 2006, 125: 201–205[23]Li H J, Conner R L, Chen Q, Jia X, Li H, Graf R J, Laroche A, Kuzyk A D. Different reactions to the wheat curl mite and wheat streak mosaic virus in various wheat–Haynaldia villosa 6V and 6VS lines. Plant Dis, 2002, 86: 423–428[24]Li H(李辉), Chen X(陈孝), Shi A-N(施爱农), Kong F-J(孔凡晶), Leath S, Murphy J P, Jia X(贾旭). Characterization of RAPD markers and RFLP marker linked to powdery mildew resistant gene derived from different H. villosa. Sci Agric Sin (中国农业科学), 2005, 38(3): 439–445 (in Chinese with English abstract)[25]Song W(宋伟), Wang F-G(王凤格), Yi H-M(易红梅), Li X(李翔), Zhao J-R(赵久然). Functional markers and their potential application in varieties identification and MAS breeding. Mol Plant Breed (分子植物育种), 2009, 7(3): 612–618 (in Chinese with English abstract)[26]Yang J(杨杰), Cao Q(曹卿), Wang J(王军), Fan F-J(范方军), Zhang Y-Q(张玉琼), Zhong W-G(仲维功). Development and application of functional markers for polyphenol oxidase(PPO) alleles in rice. Chin J Rice Sci (中国水稻科学), 2011, 25(1): 37–42 (in Chinese with English abstract)[27]Ning L-H(宁丽华), Chen T-T(陈亭亭), Liu H-H(刘怀华), Liu X(刘旭), Ma X(马侠), Cui D-Z(崔德周), Jiang C(姜川), Zhang H(张华), Wang L-W(王莉雯), Li D-T(李德涛), Chen H-B(陈化榜). Development and utilization of amylose-extender functional marker in high amylose maize. Mol Plant Breed (分子植物育种), 2011, 9(2): 185–189 (in Chinese with English abstract)[28]Yang J(杨杰), Wang J(王军), Cao Q(曹卿), Chen Z-D(陈志德), Zhong W-G(仲维功). Development and application of a functional marker for wide compatibility gene S5-n of rice. Acta Agron Sin (作物学报), 2009, 35(11): 2000–2007 (in Chinese with English abstract)[29]He D-H(贺道华), Lei Z-P(雷忠萍), Xing H-Y(邢宏宜). Development progress, characteristics and application of functional marker. J Northwest A&F Univ (Nat Sci Edn) (西北农林科技大学学报•自然科学版), 2009, 37(1): 110–116 (in Chinese with English abstract)[30]Ye X-G(叶兴国), Chen M(陈明), Du L-P(杜丽璞), Xu H-J(徐惠君). Description and evaluation of transformation approaches used in wheat. Hereditas (遗传), 2011, 33(5): 422–430 (in Chinese with English abstract) |
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