作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1833-1838.doi: 10.3724/SP.J.1006.2012.01833
党良1,2,**,王爱云1,3,**,徐惠君1,祝秀亮1,杜丽璞1,邵艳军2,张增艳1,*
DANG Liang1,2,**,WANG Ai-Yun1,3,**,XU Hui-Jun1,ZHU Xiu-Liang1,DU Li-Pu1,SHAO Yan-Jun2,ZHANG Zeng-Yan1,*
摘要:
GmPGIP3是大豆的一种多聚半乳糖醛酸酶抑制蛋白, 能够特异性地抑制部分病原真菌内切多聚半乳糖醛酸活性, 从而减弱病原菌对植株的侵害。利用基因重组技术构建了GmPGIP3基因的单子叶植物表达载体pA25-GmPGIP3, 通过基因枪介导法将pA25-GmPGIP3转入小麦品种扬麦18中。对转GmPGIP3基因扬麦18的T0至T2代植株进行PCR、Southern杂交、半定量RT-PCR和荧光定量Q-RT-PCR分析, 并对根腐病进行抗性鉴定。结果表明, GmPGIP3已转入扬麦18, 并在转基因小麦中遗传、转录和表达;比受体材料相比, 5个GmPGIP3过表达的转基因小麦株系对根腐病的抗性有明显提高。
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