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作物学报 ›› 2025, Vol. 51 ›› Issue (2): 358-369.doi: 10.3724/SP.J.1006.2025.44091

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

嗜线虫沙雷氏菌Serratia nematodiphila TG10增强油菜耐盐碱能力

苏晴芳1,2，孙小钊1,2，林杨1，付艳苹1，程家森1，谢甲涛1,2，姜道宏1,2，陈桃1,2,*   

  1. 1华中农业大学植物科学技术学院 / 农业微生物资源发掘与利用全国重点实验室, 湖北武汉430070； 2湖北洪山实验室, 湖北武汉430070

  • 收稿日期:2024-06-07 修回日期:2024-09-18 接受日期:2024-09-18 出版日期:2025-02-12 网络出版日期:2024-10-10
  • 基金资助:
    本研究由中央高校基本科研专项资金项目(X2662024ZKPY004, 2662023PY006)和财政部和农业农村部国家现代农业产业技术体系建设专项 (CARS-12)资助。

Serratia nematodiphila TG10 enhanced salt-alkali tolerance in rapeseed

SU Qing-Fang1,2,SUN Xiao-Zhao1,2,LIN Yang1,FU Yan-Ping1,CHENG Jia-Sen1,XIE Jia-Tao1,2,JIANG Dao-Hong1,2,CHEN Tao1,2,*   

  1. 1 College of Plant Science and Technology, Huazhong Agricultural University / National Key Laboratory of Agricultural Microbiology, Wuhan 430070, Hubei, China; 2 Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China
  • Received:2024-06-07 Revised:2024-09-18 Accepted:2024-09-18 Published:2025-02-12 Published online:2024-10-10
  • Supported by:
    This study was supported by the Fundamental Research Funds for the Central Universities (X2662024ZKPY004, 2662023PY006) and the China Agriculture Research System of MOF and MARA (CARS-12).

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摘要：

油菜具有较强的耐盐碱能力，本研究从天津盐碱土中分离得到一株嗜线虫沙雷氏菌Serratia nematodiphila TG10，它不仅能够在8%的NaCl和pH为10.15的R2A培养基上生长，还能定殖在油菜根系和根内。在盐胁迫条件下，TG10菌株处理后可以促进拟南芥和油菜生长。含盐1.2%基质土和哈尔滨盐碱土盆栽试验中，TG10处理后可以促进油菜鲜重和干重的增加，Na+含量显著下降，其中K+与Na+的含量比值增加；同样在天津盐碱土和吉林盐碱土盆栽试验中，TG10处理后均可显著增加油菜鲜重、叶绿素和脯氨酸的含量。吉林盐碱土盆栽油菜的转录组数据表明，TG10菌株处理后细胞色素P450代谢通路、硫代葡萄糖硫苷生物通路等显著富集，一些抗逆相关的基因显著上调表达。另外，TG10菌株能抑制油菜病原菌的生长，并且诱导油菜抗菌核病和灰霉病。本研究结果表明，嗜线虫沙雷氏菌S. nematodiphila TG10能增强油菜在不同类型盐碱土中的耐盐能力，为盐碱地的生物修复微生物菌种资源和理论支撑。

关键词: 盐碱土, 油菜, 嗜线虫沙雷氏菌, 耐盐碱机制, 诱导抗性

Abstract:

Rapeseed exhibits strong resistance to salt-alkali stress. In this study, a strain of Serratia nematodiphila TG10 was isolated from saline-alkali soil in Tianjin. This strain can not only grow on a 6% NaCl R2A medium plate with a pH of 10.15 but can also colonize the roots and rhizosphere of rapeseed. Under salt stress, the TG10 strain promoted the growth of both Arabidopsis and rapeseed. In pot experiments using a 1.2% salt-containing matrix soil and saline-alkali soil from Harbin, TG10 treatment enhanced the fresh and dry weights of rapeseed, reduced Na? content, and increased the K?/Na? ratio. In experiments with saline-alkali soils from Tianjin and Jilin, TG10 treatment significantly increased the fresh weight, chlorophyll content, and proline levels in rapeseed. Transcriptome analysis of rapeseed grown in Jilin saline-alkali soil revealed that the cytochrome P450 metabolic pathway and glucosinolate biosynthesis pathway were significantly enriched following TG10 treatment, with significant upregulation of several stress-related genes. Additionally, the TG10 strain inhibited the growth of pathogenic bacteria in rapeseed and induced resistance against Sclerotinia sclerotiorum and Botrytis cinerea. These findings suggest that S. nematodiphila TG10 can enhance the salt tolerance of rapeseed in various types of saline-alkali soils, providing a valuable resource and theoretical foundation for the biorefining of microorganisms in saline-alkali environments.

Key words: saline-alkali soil, rapeseed, Serratia nematodiphila, saline-alkali resistance mechanism, induced resistance

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