作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1818-1826.doi: 10.3724/SP.J.1006.2012.01818
葛江燕,陈士强,高营营,高勇,朱雪,黄泽峰,陈建民
GE Jiang-Yan,CHEN Shi-Qiang,GAO Ying-Ying,GAO Yong,ZHU Xue,HUANG Ze-Feng,CHEN Jian-Min*
摘要:
分别以二倍体长穗偃麦草和普通小麦中国春的基因组DNA作实验组和对照组,运用抑制消减杂交(SSH)技术去除2个基因组DNA之间的同源序列, 获得长穗偃麦草特异的DNA片段构建的单向抑制消减文库,并对随机克隆测序。根据测序结果设计引物,获得36个长穗偃麦草基因组特异分子标记,成功率高达69.2%。这些标记均能在具有长穗偃麦草E染色体的不同小麦背景和不同世代中稳定表现,可用于小麦背景中跟踪长穗偃麦草染色体或片段。
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