硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制

doi:10.3724/SP.J.1006.2021.01072

作物学报 ›› 2021, Vol. 47 ›› Issue (7): 1402-1414.doi: 10.3724/SP.J.1006.2021.01072

• 研究简报 • 上一篇

硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制

段亚梅¹^,², 罗贤磊², 陈士强³, 高勇², 陈建民¹^,²^,^*(), 戴毅¹^,^*()

¹扬州大学农业科技发展研究院 / 教育部农业与农产品安全国际联合实验室, 江苏扬州 225009
²扬州大学生物科学与技术学院 / 江苏高校现代谷物生产协作中心, 江苏扬州 225009
³江苏省里下河地区农业科学研究所, 江苏扬州 225009

收稿日期:2020-09-04 接受日期:2020-12-02 出版日期:2021-07-12 网络出版日期:2020-12-28
通讯作者: 陈建民,戴毅
作者简介:E-mail: 834630650@qq.com
基金资助:
本研究由江苏省自然科学基金项目(BK20180908);本研究由江苏省自然科学基金项目(BK20181213);扬州市自然科学基金项目资助(YZ2018097)

Creation of disomic addition, substitution and translocation lines of durum wheat-Thinopyrum elongatum

DUAN Ya-Mei¹^,², LUO Xian-Lei², CHEN Shi-Qiang³, GAO Yong², CHEN Jian-Min¹^,²^,^*(), DAI Yi¹^,^*()

¹Institute of Agricultural Science and Technology Development / Joint International Research Laboratory of Agriculture & Agri-Product Safety of MOE, Yangzhou University, Yangzhou 225009, Jiangsu, China;
²College of Bioscience and Biotechnology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China
³Institute of Agricultural Sciences, Lixia River Region, Yangzhou 225009, Jiangsu, China

Received:2020-09-04 Accepted:2020-12-02 Published:2021-07-12 Published online:2020-12-28
Contact: CHEN Jian-Min,DAI Yi
Supported by:
This research was supported by the Natural Science Foundation of Jiangsu(BK20180908);This research was supported by the Natural Science Foundation of Jiangsu(BK20181213);the Natural Science Foundation of Yangzhou(YZ2018097)

摘要/Abstract

摘要：

通过小麦与长穗偃麦草远缘杂交创制附加系、代换系及易位系是小麦遗传改良中利用长穗偃麦草优良基因的重要途径。本研究将长穗偃麦草特异分子标记、染色体计数、基因组原位杂交(GISH)及非变性原位杂交(ND-FISH)等多种方法相结合, 对硬粒小麦Langdon (AABB)与小偃麦8801 (AABBEE)的杂交后代群体进行分子细胞学鉴定, 创制出硬粒小麦-长穗偃麦草3E、6E染色体双体附加系Du-DA3E和Du-DA6E, 硬粒小麦-长穗偃麦草1E (1B)染色体双体代换系Du-DS1E (1B)以及硬粒小麦-长穗偃麦草1AS-1EL染色体易位系Du-T1AS·1EL。创制的4个种质中长穗偃麦草染色体均能稳定遗传, 这不仅增加了硬粒小麦-长穗偃麦草附加系和代换系的类型, 还为后续利用长穂偃麦草优良基因改良小麦提供了特殊种质资源。

关键词: 硬粒小麦, 长穗偃麦草, 附加系, 代换系, 易位系

Abstract:

The establishment of chromosome addition, substitution and translocation lines through distant hybridization between wheat and Thinopyrum elongatum is an important approach to utilize useful genes of Th. elongatum in wheat improvement. Based on chromosome-specific molecular markers, chromosome counting, genome in situ hybridization (GISH), and non-denaturing fluorescence in situ hybridization (ND-FISH), several stable addition, substitution or translocation lines were identified from a cross between Triticum turgidum ssp. durum Langdon (AABB) and Trititrigia 8801 (AABBEE). Two 3E and 6E disomic addition lines Du-DA3E and Du-DA6E of durum wheat-Th. elongatum, a 1E (1B) disomic substitution line Du-DS1E (1B), and a 1AS-1EL disomic translocation line Du-T1AS·1EL were created in this study. The chromosome-segments of Th. elongatum could be inherited stably in these new lines, which not only increased the types of addition and substitution lines of durum wheat-Th. elongatum, but also provided special germplasm resources for the follow-up use of excellent genes of Th. elongatum in wheat improvement.

Key words: Triticum turgidum, Thinopyrum elongatum, addition line, substitution line, translocation line

段亚梅, 罗贤磊, 陈士强, 高勇, 陈建民, 戴毅. 硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制[J]. 作物学报, 2021, 47(7): 1402-1414.

DUAN Ya-Mei, LUO Xian-Lei, CHEN Shi-Qiang, GAO Yong, CHEN Jian-Min, DAI Yi. Creation of disomic addition, substitution and translocation lines of durum wheat-Thinopyrum elongatum[J]. Acta Agronomica Sinica, 2021, 47(7): 1402-1414.

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参考文献 54

[1]	赵鹏, 秦明波. 植物染色体工程与育种. 生物学通报, 1996, 31(7): 4‒7.
	Zhao P, Qin M B. Plant chromosome engineering and breeding. Bull Biol, 1996,31(7):4-7 (in Chinese).
[2]	王林生, 陈佩度. 普通小麦外源染色体易位系的诱导及其在育种上的应用. 生物学通报, 2007,42(2):9-11.
	Wang L S, Chen P D. Induction of aline chromosome translocation lines in common wheat and its application in breeding. Bull Biol, 2007,42(2):9-11 (in Chinese).
[3]	刘大钧, 陈佩度, 吴沛良, 王耀南, 邱伯行, 王苏玲. 硬粒小麦-簇毛麦双二倍体. 作物学报, 1986,12:155-162.
	Liu D J, Chen P D, Wu P L, Wang Y N, Qiu B X, Wang S L. Durum wheat- Dasypyrum villosum diploid. Acta Agron Sin, 1986,12:155-162 (in Chinese with English abstract).
[4]	韩方普, 李集临. 硬粒小麦、提莫菲维小麦与四倍体长穗偃麦草属间杂种的形态和细胞遗传学研究. 遗传学报, 1993,20:44-49.
	Han F P, Li J L. Morphological and cytogenetic studies on hybrids of Triticum turgidum, Triticum timopheevii and tetraploid Thiuopyrum elougatum. Acta Genet Sin, 1993,20:44-49 (in Chinese with English abstract).
[5]	Guo X, Shi Q H, Wang J, Hou Y L, Wang Y H, Han F P. Characterization and genome changes of new amphiploids from wheat wide hybridization. J Genet Genom, 2015,42:459-461.
[6]	王洪刚, 刘树兵, 李兴锋, 高居荣, 封德顺, 陈冬花. 六个八倍体小偃麦的选育和鉴定. 麦类作物学报, 2006,26(4):6-10.
	Wang H G, Liu S B, Li X F, Gao J R, Feng D S, Chen D H. Breeding and identification of six octoploid Trititrigia. J Triticeae Crops, 2006,26(4):6-10 (in Chinese with English abstract).
[7]	丁志远. 小麦异附加系种质资源的开发利用初探. 甘肃农业科技, 2006, ( 5):15-17.
	Ding Z Y. Preliminary study on utilization of alien chromosome addition lines of wheat. Gansu Agric Sci Technol, 2006, ( 5):15-17 (in Chinese with English abstract).
[8]	Li X J, Hu X G, Hu T Z, Li G, Ru Z G, Zhang L L, Lang Y M. Identification of a novel wheat- Thinopyrum ponticum addition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers. Cereal Res Commun, 2015,43:1-10.
[9]	Liu H P, Dai Y, Chi D, Huang S, Li H F, Duan Y M, Cao W G, Gao Y, Fedak G, Chen J M. Production and molecular cytogenetic characterization of a durum wheat- Thinopyrum elongatum 7E disomic addition line with resistance to Fusarium head blight. Cytogenet Genome Res, 2018,153:165-173. doi: 10.1159/000486382 pmid: 29421795
[10]	穆素梅, 钟冠昌, 李振声, 李滨. 利用缺体回交法选育小黑麦异代换系的研究. 中国生态农业学报, 1996,4(1):33-36.
	Mu S M, Zhong G C, Li Z S, Li B. Study on breeding of different substitution lines of Triticale by backcrossing. Chin J Eco-Agric, 1996,4(1):33-36 (in Chinese with English abstract).
[11]	Hu L J, Li G R, Zeng Z X, Chang Z J, Liu C, Zhou J P, Yang Z J. Molecular cytogenetic identification of a new wheat- Thinopyrum substitution line with stripe rust resistance. Euphytica, 2011,177:169-177.
[12]	李桂英, 王琳清, 施巾帼. 辐照花粉对普通小麦×窄颖赖草杂种的细胞学效应. 核农学报, 2000,14:6-11.
	Li G Y, Wang L Q, Shi J G. Cytological effects of irradiated pollen on the hybrid of Triticum aestivum × Leymus angustus. Acta Agric Nucl Sin, 2000,14:6-11 (in Chinese with English abstract).
[13]	李洪杰, 郭北海, 张艳敏, 李义文, 杜立群, 李银心, 贾旭, 朱至清. 利用组织培养和辐射诱变创造高频率小麦与簇毛麦染色体易位. 遗传学报, 2000,27:511-519.
	Li H J, Guo B H, Zhang Y M, Li Y W, Du L Q, Li Y X, Jia X, Zhu Z Q. Using tissue culture and radiation mutagenesis to create high-frequency chromosomal translocation between Triticum aestivum and Dasypyrum villosum. Acta Genet Sin, 2000,27:511-519 (in Chinese with English abstract).
[14]	张璐璐, 陈士强, 李海凤, 刘慧萍, 戴毅, 高勇, 陈建民. 小麦-长穗偃麦草7E抗赤霉病易位系培育. 中国农业科学, 2016,49:3477-3488.
	Zhang L L, Chen S Q, Li H F, Liu H P, Dai Y, Gao Y, Chen J M. Development of Wheat- Thinopyrum elongatum translocation lines resistant to Fusarium head blight. Sci Agric Sin, 2016,49:3477-3488 (in Chinese with English abstract).
[15]	Friebe B, Mukai Y, Dhaliwal H S, Martin T J, Gill B S. Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization. Theor Appl Genet, 1991,81:381-389. doi: 10.1007/BF00228680 pmid: 24221269
[16]	Friebe B, Jiang J, Knott D R, Gill B S. Compensation indices of radiation-induced wheat- Agropyron elongatum translocations conferring resistance to leaf rust and stem rust. Crop Sci, 1994,34:400-404.
[17]	Li H J, Conner R L, Chen Q, Li H Y, Laroche A, Graf R J, Kuzyk A D. The transfer and characterization of resistance to common root rot from Thinopyrum ponticum to wheat. Genome, 2004,47:215-223. doi: 10.1139/g03-095 pmid: 15060618
[18]	Fu S L, Lyu Z L, Qi B, Guo X, Li J, Liu B, Han F P. Molecular cytogenetic characterization of wheat- Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat fusarium head blight. J Genet Genomics, 2012,39:103-110.
[19]	He F, Xu J Q, Qi X L, Wang H G. Molecular cytogenetic characterization of two partial wheat Elytrigia elongatum amphiploids resistant to powdery mildew. Plant Breed, 2013,132:553-557.
[20]	Zheng Q, Klindworth D L, Friesen T L, Liu A F, Li Z S, Zhong S B, Jin Y, Xu S S. Characterization of Thinopyrum species for wheat stem rust resistance and ploidy level. Crop Sci, 2014,54:2663-2672.
[21]	Wang H W, Sun S L, Ge W Y, Zhao L F, Hou B Q, Wang K, Lyu Z F, Chen L Y, Xu S S, Guo J, Li M, Su P S, Li S F, Wang G P, Bo C Y, Fang X J, Zhuang W W, Cheng X X, Wu J W, Dong L H, Chen W Y, Li W, Xiao G L, Zhao J X, Hao Y C, Xu Y, Gao Y, Liu W J, Liu Y H, Yin H Y, Li J Z, Li X, Zhao Y, Wang X Q, Ni F, Ma X, Li A F, Xu S S, Bai G H, Nevo E, Gao C X, Ohm H, Kong L R,. Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science, 2020, 368: eaba5435. doi: 10.1126/science.aba5435 pmid: 32273397
[22]	Omielan J A, Epstein E, Dvořák J. Salt tolerance and ionic relations of wheat as affected by individual chromosomes of salt-tolerant Lophopyrum elongatum. Genome, 1991,34:961-974.
[23]	Taeb M, Koebner R M, Forster B P. Genetic variation for waterlogging tolerance in the Triticeae and the chromosomal location of genes conferring waterlogging tolerance in Thinopyrum elongatum. Genome, 1993,36:825-830. doi: 10.1139/g93-110 pmid: 18470030
[24]	孟林, 杨宏新, 毛培春, 高洪文. 偃麦草属植物种间苗期抗旱性评价. 草业学报, 2011,20(5):34-41.
	Meng L, Yang H X, Mao P C, Gao H W. Evaluation of drought resistance of Thinopyrum plants in seedling stage. Acta Pratacult Sin, 2011,20(5):34-41 (in Chinese with English abstract).
[25]	李振声. 创造新物种与改良旧物种—远缘杂交在作物育种中的作用. 中国农业科学, 1977,10(3):38-43.
	Li Z S. Creating new species and improving old species-The role of distant hybridization in crop breeding. Sci Agric Sin, 1977,10(3):38-43 (in Chinese).
[26]	Wang R R C, Lu B R. Biosystematics and evolutionary relationships of perennial Triticeae species revealed by genomic analyses. J Syst Evol, 2014,52:697-705.
[27]	刘登才, 郑有良, 王志容, 侯永翠, 兰秀锦, 魏育明. 影响小麦赤霉病抗性的Lophopyrum elongatum染色体定位. 四川农业大学学报, 2001, 19(3): 200‒205.
	Liu D C, Zhen Y L, Wang Z R, Huo Y C, Lan X J, Wei Y M. Chromosomal location of Lophopyrum elongatum affecting FHB resistance of wheat. J Sichuan Agric Univ, 2001,19(3):200-205 (in Chinese with English abstract).
[28]	Shen X, Kong L, Ohm H. Fusarium head blight resistance in hexaploid wheat ( Triticum aestivum)-Lophopyrum, genetic lines and tagging of the alien chromatin by PCR markers. Theor Appl Genet, 2004,108:808-813. doi: 10.1007/s00122-003-1492-9 pmid: 14628111
[29]	Shen X, Ohm H. Fusarium head blight resistance derived from Lophopyrum elongatum, chromosome 7E and its augmentation with Fhb1 in wheat. Plant Breed, 2006,125:424-429.
[30]	Jauhar P P, Peterson T S. Cytological and molecular characterization of homoeologous group-1 chromosomes in hybrid derivatives of a durum disomic alien addition line. Plant Genome, 2011,4:102-109.
[31]	Jauhar P P, Peterson T S. Registration of DGE-2, a durum wheat disomic alien substitution line 1E(1A) involving a diploid wheatgrass chromosome. J Plant Regist, 2012,6:221-223.
[32]	Jauhar P P, Peterson T S. Registration of DGE-3, a durum wheat disomic substitution line 1E(1B) involving a wheatgrass chromosome. J Plant Regist, 2013,7:257-259.
[33]	Anderson J M, Bucholtz D L, Sardesai N, Santini J B, Gyulai G, Williams C E, Goodwin S B. Potential new genes for resistance to Mycosphaerella graminicola identified in Triticum aestivum × Lophopyrum elongatum disomic substitution lines. Euphytica, 2010,172:251-262.
[34]	Dewey D R. Salt tolerance of twenty-five strains of Agropyron. Agron J, 1960,52:631-635.
[35]	Zhong G Y, Dvorak J. Chromosomal control of the tolerance of gradually and suddenly imposed salt stress in the Lophopyrum elongatum and wheat, Triticum aestivum L. genomes. Theor Appl Genet, 1995,90:229-236. doi: 10.1007/BF00222206 pmid: 24173895
[36]	王景雪, 孙毅, 高武军. 一种简便实用的植物总DNA提取方法. 山西大学学报, 2000,23:271-272.
	Wang J X, Sun Y, Gao W J,. A simple and practical method for extracting plant total DNA. J Shanxi Univ, 2000, 23: 271‒272 (in Chinese with English abstract).
[37]	Chen S Q, Huang Z F, Dai Y, Qin S W, Gao Y Y, Zhang L L, Gao Y, Chen J M. The development of 7E chromosome-specific molecular markers for Thinopyrum elongatum based on SLAF-seq technology. PLoS One, 2013,8:e65122.
[38]	罗贤磊, 戴毅, 陶海霞, 陈士强, 李海凤, 段亚梅, 高勇, 陈建民. 硬粒小麦-长穗偃麦草2E和4E附加系及E染色体传递. 西北植物学报, 2019,39:953-962.
	Luo X L, Dai Y, Tao H X, Chen S Q, Li H F, Duan Y M, Gao Y, Chen J M. Durum- Thinopyrum elongatum 2E and 4E addition lines and E chromosome transmission. Acta Bot Boreali-Occident Sin, 2019,39:953-962 (in Chinese with English abstract).
[39]	Kato A, Lamb J C, Birchler J A. Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci USA, 2004,101:13554-13559. doi: 10.1073/pnas.0403659101 pmid: 15342909
[40]	Tang Z X, Yang Z J, Fu S L. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet, 2014,55:313-318. pmid: 24782110
[41]	顾爱侠, 李雪姣, 冯大领, 王彦华, 陈雪平, 申书兴. 基因组原位杂交中封阻DNA制备方法研究. 河北农业大学学报, 2010,33(5):77-79.
	Gu A X, Li X J, Feng D L, Wang Y H, Chen X P, Shen S Z. Preparation of blocking DNA in genomic in situ hybridization. J Agric Univ Hebei, 2010,33(5):77-79 (in Chinese with English abstract).
[42]	侯贤清, 贾志宽, 韩清芳, 王维, 丁瑞霞, 聂俊峰, 李永平. 轮耕对宁南旱区冬小麦花后旗叶光合性能及产量的影响. 中国农业科学, 2011,44:3108-3117.
	Hou X Q, Jia Z K, Han Q F, Wang W, Ding R X, Nie J F, Li Y P. Effects of rotational tillage on flag leaf photosynthetic characteristics and yield after anthesis of winter wheat in arid areas of southern Ningxia. Sci Agric Sin, 2011, 44: 3108‒3117 (in Chinese with English abstract).
[43]	Li D Y, Li T H, Wu Y L, Zhang X H, Zhu W, Wang Y, Zeng J, Xu L L, Fan X, Sha L, Zhang H Q, Zhou Y H, Kang H Y. FISH-Based markers enable identification of chromosomes derived from tetraploid Thinopyrum elongatum in hybrid lines. Front Plant Sci, 2018,9:526. doi: 10.3389/fpls.2018.00526 pmid: 29765383
[44]	刘树兵, 王洪刚. 抗白粉病小麦-中间偃麦草(Thinopyrum intermedium, 2n = 42)异附加系的选育及分子细胞遗传鉴定. 科学通报, 2002,47:1500-1503.
	Liu S B, Wang H G. Breeding and molecular cytogenetic identification of powdery mildew resistant wheat- Thinopyrum intermedium (Thinopyrum intermedium, 2n = 42) addition lines. Chin Sci Bull, 2002,47:1500-1503 (in Chinese).
[45]	Garg M, Tanaka H, Ishikawa N, Takata K, Yanaka M, Tsujimoto H. Agropyron elongatum HMW-glutenins have a potential to improve wheat end-product quality through targeted chromosome introgression. J Cereal Sci, 2009,50:358-363.
[46]	张学勇. 普通小麦异源易位系的产生及利用. 遗传, 1991,13(5):39-44.
	Zhang X Y. Production and utilization of heterologous translocation lines in common wheat. Hereditas, 1991,13(5):39-44 (in Chinese).
[47]	戴毅. 小麦-黑麦-偃麦草三属杂种种质创制及分子细胞遗传学研究. 扬州大学博士学位论文, 江苏扬州, 2017.
	Dai Y. Development of Germplasms for Triticum-Secale- Thinopyrum trigeneric Hybrids and Molecular Cytogenetic Research. PhD Dissertation of Yangzhou University, Yangzhou, Jiangsu, China, 2017 (in Chinese with English abstract).
[48]	Linc G, Sepsi A, Molnár-láng M. A FISH karyotype to study chromosome polymorphisms for the Elytrigia elongatum E genome. Cytogenet Genome Res, 2012,136:138-144. doi: 10.1159/000334835 pmid: 22188811
[49]	李海凤, 刘慧萍, 戴毅, 黄帅, 张军, 高勇, 陈建民. 四倍体小麦背景中长穗偃麦草E染色体传递特征. 遗传, 2016,38:1021-1030.
	Li H F, Liu H P, Dai Y, Huang S, Zhang J, Gao Y, Chen J M. Transmitting characters of individual E chromosomes of Thinopyrum elongatum in Triticum turgidum background. Hereditas, 2016,38:1021-1030 (in Chinese with English abstract).
[50]	Ceoloni C, Forte P, Gennaro A, Micali S, Carozza R, Bitti A. Recent developments in durum wheat chromosome engineering. Cytogenet Genome Res, 2005,109:328-344. pmid: 15753593
[51]	谢枫, 吴跃进, 杨阳, 倪晓宇, 余立祥, 刘斌美, 王钰. 不同小麦品种籽粒产量对低氮胁迫的响应及其与旗叶光合特性的关系. 生物学杂志, 2018,35(6):42-45.
	Xie F, Wu Y J, Yang Y, Ni X Y, Yu L X, Liu B M, Wang Y. Yield response of wheat cultivars to low nitrogen stress and its relationship with photosynthetic traits in flab leaves. J Biol, 2018,35(6):42-45 (in Chinese with English abstract).
[52]	陈士强, 黄泽峰, 戴毅, 秦树文, 高营营, 高勇, 陈建民. 长穗偃麦草E组染色体对小麦光合速率和产量性状的效应. 扬州大学学报, 2013,34(1):50-55.
	Chen S Q, Huang Z F, Dai Y, Qin S W, Gao Y Y, Gao Y, Chen J M. Effect of E chromosomes of Thinopyrum elongatum on photosynthesis and yield characters in wheat. J Yangzhou Univ, 2013, 34(1): 50‒55 (in Chinese with English abstract).
[53]	陈士强, 何震天, 张容, 王建华, 王锦荣, 陈建民, 陈秀兰. 长穗偃麦草优异基因的染色体定位及应用. 植物遗传资源学报, 2015,16:1062-1066.
	Chen S Q, He Z T, Zhang R, Wang J H, Wang J R, Chen J M, Chen X L. Chromosomal localization of excellent genes in Thinopyrum elongatum and Thinopyrum ponticum and their application in wheat improvement. J Plant Genet Resour, 2015,16:1062-1066 (in Chinese with English abstract).
[54]	Kumar A, Garg M, Kaur N, Chunduri V, Sharma S, Misser S, Kumar A, Tsujimoto H, Dou Q W, Gupta R K. Rapid development and characterization of chromosome specific translocation line of Thinopyrum elongatum with improved dough strength. Front Plant Sci, 2017,8:1593. doi: 10.3389/fpls.2017.01593 pmid: 28959271

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材料名称 Material name	染色体数 Chromosome number	染色体组成 Chromosome composition	缩写 Abbreviations
硬粒小麦(Langdon) Triticum turgidum ssp. durum (Langdon)	2n=28	AABB	Ld
六倍体小偃麦8801 Hexaploid Trititrigia	2n=28+14=42	AABBEE	8801
二倍体长穗偃麦草 Diploid Th. elongatum	2n=14	E^eE^e	2X
四倍体长穗偃麦草 Tetraploid Th. elongatum	2n=28	E^e₁E^e₁E^e₂E^e₂	4X
普通小麦中国春 Common wheat Chinese spring	2n=42	AABBDD	CS
中国春-长穗偃麦草1E-7E附加系 Chinese spring-Th. elongatum 1E-7E addition lines	2n=44	AABBDD+II^1E-7E	DA1E~7E
中国春-长穂偃麦草1EL端体附加系 Chinese spring-Th. elongatum 1EL telosomic addition line	2n=44	AABBDD+II^1EL	DA1EL
中国春-长穂偃麦草1ES端体附加系 Chinese spring-Th. elongatum 1ES telosomic addition line	2n=44	AABBDD+II^1ES	DA1ES
硬粒小麦-长穗偃麦草1E(1B)代换系 Durum wheat-Th. elongatum 1E(1B) substitution line	2n=26+2=28	AABB+II^1E(1B)	Du-DS1E(1B)
硬粒小麦-长穗偃麦草3E附加系 Durum wheat-Th. elongatum 3E addition line	2n=28+2=30	AABB+II^3E	Du-DA3E
硬粒小麦-长穗偃麦草6E附加系 Durum wheat-Th. elongatum 6E addition line	2n=28+2=30	AABB+II^6E	Du-DA6E
硬粒小麦-长穗偃麦草1AS-1EL易位系 Durum wheat-Th. elongatum 1AS-1EL translocation line	2n=26+2T=28	AABB+ II^1AS·^1EL	Du-T1AS·1EL

标记名称 Molecular marker	引物序列Primer sequence (5'-3')		扩增染色体 Amplified chromosome	参考文献 Reference
标记名称 Molecular marker	正向Forward	反向Reverse	扩增染色体 Amplified chromosome	参考文献 Reference
GSM-1	AATCAGTCATAGTATCCAAAGTCTCCG	TGTTTAGAATAGGATGGGTAGAGC	1E-7E	This study
GSM-2	TCTTACAGATAGGTCCCTGGCAT	TGGTAGTCACCTATCCAAGAAT	1E-7E	This study
GSM-3	TGTTTCTTAGTTGTTTTGTT	GCCTTGACCACCATAC	1E-7E	Chen et al. ^[37]
CSM-1E-1	GATCTGTTTACGTCGATGCCG	TGCCGAACTATTTGCTCCTTG	1E, 1EL	This study
CSM-1E-2	TGAGAGGCGTGAGGACCTTGCTAAT	GGTTTCCCAAGTCTTGTGATGC	1E, 1ES	This study
CSM-1E-3	AGGTTCATAAGAATCCATC	TACTGGATTTGTCACTTGCT	1E, 1EL	This study
CSM-1E-4	ATGTCAGCCTATCCTACGGGTGT	ATTCGTTGGGTCACTTGTCTCCT	1E, 1ES	This study
CSM-1E-5	TCCCAACCACCTTCGCATTCATT	GGCGAAAGACCATCAAAAGGAGTG	1E, 1EL	This study
CSM-1E-6	CGTCACCAGAGAATAACAGGAG	GTTCTATGTCAAGCAGTTCCG	1E, 1EL	This study
CSM-1E-7	CCCGTGTAAGGAACAGAAAC	TTAAGGTCGTCCCAACAGG	1E, 1EL	This study
CSM-2E-1	TCAATGTCAAGGAGGAGGGTC	TGGTCAAGAAGACAAGGGAAG	2E	This study
CSM-3E-1	TATTAGTCGAGCACCTGTCG	ATCTTCCTTGGCATCCTTC	3E	This study
CSM-3E-2	CTGAGGGTTTAATTCGTAGCG	TGATGGTTTCCCACTGTTCC	3E	This study
CSM-3E-3	AGAGGATTTGAGGCTGTTCG	AGATGTGCCGTGTAAAGGAGT	3E	This study
CSM-3E-4	TGATGAGTCGAGCAAACAACC	ATGTCCCTAAAGCCAAGTCC	3E	This study
CSM-3E-5	TACTCCATAGAGGCTAGAAGAAC	TGTCAAAGGCAAGCAAAGC	3E	This study
CSM-4E-1	GAGAAGCCTCTGCCTTTAAG	ACGGCGAGTCCTTGGTC	4E	Luo et al. ^[38]
CSM-5E-1	ACCATTAGGTCACCGCAGTCT	CGTTCTCGTGTCCCTACTCTTGT	5E	This study
CSM-6E-1	GAGTGCTGGGATGAAGAAG	GACGAAGACTAGGCACCAG	6E	This study
CSM-6E-2	GCTAATGAGGGTTACAGAGT	TAAAGAGCATCGGGGAC	6E	This study
CSM-6E-3	AGCTGGCTCATCCCCATTTCT	CGTGTTTACTGCCCATTTGTCC	6E	This study
CSM-6E-4	TTTTTCGCACCACCCTTTC	TTGTTCGAGAACGGGTTGC	6E	Luo et al. ^[38]
CSM-6E-5	ACGAGGTTTGGCCGGTAT	TACTCTTTGCTGATGATAGGCTC	6E	Luo et al. ^[38]
CSM-7E-1	CTGCGGTTCTAAAATACTGAGG	AAAGCTTGCCAAATGCC	7E	This study

标记名称 Molecular marker	扩增染色体 Amplified chromosome	Du_No.1	Du_No.3	Du_No.6	Du_No.8
CSM-1E-1	1E, 1EL	+	-	-	+
CSM-2E-1	2E	-	-	-	-
CSM-3E-1	3E	-	+	-	-
CSM-4E-1	4E	-	-	-	-
CSM-5E-1	5E	-	-	-	-
CSM-6E-1	6E	-	-	+	-
CSM-7E-1	7E	-	-	-	-
CSM-1E-2	1E, 1ES	+	*	*	-
CSM-1E-3	1E, 1EL	+	*	*	+
CSM-1E-4	1E, 1ES	+	*	*	-
CSM-1E-5	1E, 1EL	+	*	*	+
CSM-1E-6	1E, 1EL	+	*	*	+
CSM-1E-7	1E, 1EL	+	*	*	+
CSM-3E-2	3E	*	+	*	*
CSM-3E-3	3E	*	+	*	*
CSM-3E-4	3E	*	+	*	*
CSM-3E-5	3E	*	+	*	*
CSM-6E-2	6E	*	*	+	*
CSM-6E-3	6E	*	*	+	*
CSM-6E-4	6E	*	*	+	*
CSM-6E-5	6E	*	*	+	*

材料 Materials	调查株数 Number of plants	株高 Plant height (cm)	穗长 Spike length (cm)	小穗粒数 Kernel number per spikelet	每穗小穗数 Spikelet number per spike	每穗粒数 Kernel number per spike	千粒重 1000-kernel weight (g)	旗叶面积 Flag leaf area (cm²)
Langdon	22	169.8±10.4 d	9.7±1.0 ab	2.3±0.2 bc	24.7±1.9 bc	56.1±7.3 c	37.2±1.4 d	30.6±4.7 a
8801	18	178.6±13.3 d	18.4±1.6 e	2.1±0.3 ab	20.9±2.7 a	43.6±4.5 ab	18.0±3.1 a	47.4±8.8 bc
Du-DA3E	10	95.1±15.1 a	9.3±0.5 a	2.6±0.4 c	21.0±2.5 a	53.9±7.7 bc	25.2±4.9 b	42.3±13.9 b
Du-DA6E	10	117.1±10.3 b	12.8±0.9 d	3.5±0.3 d	26.4±2.5 c	93.1±10.0 d	30.7±3.1 c	57.2±8.0 c
Du-DS1E (1B)	10	109.1±11.8 b	12.0±2.0 cd	1.8±0.6 a	23.7±2.1 b	42.2±16.9 a	20.1±3.3 a	48.1±9.6 bc
Du-T1AS·1EL	12	134.8±7.7 c	10.7±0.8 bc	2.5±0.4 bc	23.6±1.4 b	58.9±9.9 c	22.5±2.6 ab	54.1±4.2 c

硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制

Creation of disomic addition, substitution and translocation lines of durum wheat-Thinopyrum elongatum

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