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作物学报 ›› 2018, Vol. 44 ›› Issue (04): 522-532.doi: 10.3724/SP.J.1006.2018.00522

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于SSSL群体的玉米穗下节间长QTL分析

郭海平1(), 孙高阳1, 张晓祥2, 闫鹏帅1, 刘坤1, 谢惠玲1, 汤继华1, 丁冬1, 李卫华1,*()   

  1. 1河南农业大学农学院 / 省部共建小麦玉米作物学国家重点实验室, 河南郑州450002
    2四川农业大学玉米研究所, 四川成都 611130
  • 收稿日期:2017-06-14 接受日期:2017-11-21 出版日期:2018-04-12 网络出版日期:2017-12-01
  • 通讯作者: 李卫华
  • 作者简介:

    guohaiping885@126.com

  • 基金资助:
    本研究由国家自然科学基金项目(U1604231), 河南省重大科技专项(161100110500)和河南省科技攻关项目(152102110060)资助

QTL Analysis of Under-ear Internode Length Based on SSSL Population

Hai-Ping GUO1(), Gao-Yang SUN1, Xiao-Xiang ZHANG2, Peng-Shuai YAN1, Kun LIU1, Hui-Ling XIE1, Ji-Hua TANG1, Dong DING1, Wei-Hua LI1,*()   

  1. 1 College of Agronomy, Henan Agricultural University / Key Laboratory of Wheat and Maize Crops Science, Zhengzhou 450002, Henan, China
    2 Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, Sichuan, China
  • Received:2017-06-14 Accepted:2017-11-21 Published:2018-04-12 Published online:2017-12-01
  • Contact: Wei-Hua LI
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (U1604231), the Major Science and Technology Projects in Henan Province (161100110500), and Henan Science and Technology Project (152102110060).

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摘要：

穗下节间长决定着玉米的穗位高和株高, 而株高和穗位高与产量、抗倒伏性等重要农艺性状密切相关。玉米穗下第7、第8、第9节间长对穗位高具有决定作用, 与其他农艺性状相比, 对穗下节间长的遗传基础了解甚少。因此, 研究玉米穗下节间长的遗传基础, 对玉米育种有重要意义。本研究以lx9801为受体亲本, 昌7-2为供体亲本通过连续回交和自交所构建的一套包含260份染色体单片段代换系的群体为研究对象, 通过两年两环境的表型鉴定, 并结合基因型数据对第7、第8、第9节间长和穗位高的QTL进行定位。共检测到18个第7节间长QTL, 23个第8节间长QTL和17个第9节间长QTL, 其中8个QTL是为第7、第8、第9节间长所共有。穗位高定位到的20个QTL中, 有12个(60%)与第7、第8、第9节间长QTL共定位。说明第7、第8、第9节间长与穗位高有着共同的遗传基础, 节间长也是穗位高的重要构成因子, 决定着玉米的株高和穗位高。

关键词: 玉米, 单片段代换系, 穗下节间长, 穗位高, 数量性状位点

Abstract:

The under-ear internode length determines maize plant height and ear height, which are two agronomic traits associated with yield and lodging resistance. The lengths of the 7th, 8th, and 9th internode play a decisive role in ear height. Compared with other agronomic traits, there is little knowledge for genetic basis of under-ear internode length. Thus, exploring the genetic basis of internode length plays an important role in maize breeding. In this study, a set of 260 chromosome segment substitution lines (CSSLs), using Chang 7-2 as the donor parent and lx9801 as recipient parent, was constructed and used to map QTLs for the 7th, 8th, and 9th internode length and ear height at two-environments in two years. In total, 18, 23, and 17 QTLs were detected for the 7th, 8th, and 9th internode length, respectively. Among them, eight QTLs were simultaneously detected for the 7th, 8th, and 9th internode length. For ear height, 20 QTLs were detected, 12 (60%) of these 20 QTLs were found to co-localize to the 7th, 8th, and 9th internode length. The results indicated that length of the 7th, 8th, and 9th internode and ear height have same genetic basis. Furthermore, length of the 7th, 8th, and 9th internode are important components of ear height and also determining the plant height and ear height in maize.

Key words: maize, single segment substitution lines (SSSL), under-ear internode length, ear height, quantitative trait loci (QTL)

表1

亲本及SSSL群体第7、第8、第9节间长及穗位高田间表现"

表2

两亲本间第7、第8、第9节间长及穗位高的t测验"

性状
Trait		 2012 2013
浚县 Xunxian 长葛 Changge 浚县 Xunxian 长葛 Changge
第7节间长7th internode length 4.047** 3.036** 0.045* 0.874**
第8节间长8th internode length 0.626** 2.145** 2.443** 2.991**
第9节间长9th internode length 6.024** 8.374** 2.967** 6.889**
穗位高Ear height 1.171** 2.609* 2.369* 2.085**

表3

SSSL群体第7、第8、第9节间长与穗位高相关系数"

年份
Year		 性状
Trait		 浚县 Xunxian 长葛 Changge
第7节间长
7th IL		 第8节间长
8th IL		 第9节间长
9th IL		 穗位高
EH		 第7节间长
7th IL		 第8节间长
8th IL		 第9节间长
9th IL		 穗位高
EH
2012 第7节间长7th IL 1 1
第8节间长8th IL 0.760** 1 0.633** 1
第9节间长9th IL 0.597** 0.635** 1 0.558** 0.538** 1
穗位高EH 0.432** 0.455** 0.439** 1 0.415** 0.443** 0.431** 1
2013 第7节间长7th IL 1 1
第8节间长8th IL 0.684** 1 0.645** 1
第9节间长9th IL 0.680** 0.677** 1 0.364** 0.482** 1
穗位高EH 0.398** 0.417** 0.403** 1 0.386** 0.412** 0.393** 1

表4

在SSSL群体中检测到第7、第8、第9节间长与穗位高QTLs"

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