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作物学报

作物学报 ›› 2019, Vol. 45 ›› Issue (11): 1615-1627.doi: 10.3724/SP.J.1006.2019.91009

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

小麦转录因子基因TaNAC67参与调控穗长和每穗小穗数

张宏娟1,2,李玉莹2,3,苗丽丽2,王景一2,李超男2,杨德龙1,*(),毛新国1,2,*(),景蕊莲2   

  1. 1 甘肃农业大学生命科学技术学院, 甘肃兰州 730070
    2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部农作物种质资源创新与利用重点开放实验室, 北京 100081
    3 河南农业大学农学院, 河南郑州 450002
  • 收稿日期:2019-01-23 接受日期:2019-05-12 出版日期:2019-11-12 网络出版日期:2019-05-22
  • 通讯作者: 杨德龙,毛新国
  • 作者简介:E-mail: 1412360690@qq.com
  • 基金资助:
    本研究由国家重点研发计划项目(2017YFD0300202);甘肃省现代农业产业技术体系项目(GARS-01-04)

Transcription factor gene TaNAC67 involved in regulation spike length and spikelet number per spike in common wheat

ZHANG Hong-Juan1,2,LI Yu-Ying2,3,MIAO Li-Li2,WANG Jing-Yi2,LI Chao-Nan2,YANG De-Long1,*(),MAO Xin-Guo1,2,*(),JING Rui-Lian2   

  1. 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China
    2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing 100081, China
    3 College of Agriculture, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2019-01-23 Accepted:2019-05-12 Published:2019-11-12 Published online:2019-05-22
  • Contact: De-Long YANG,Xin-Guo MAO
  • Supported by:
    This study was supported by the National Key Research and Development Plan(2017YFD0300202);Gansu Agriculture Research System(GARS-01-04)

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摘要：

NAC转录因子是植物特有的一类转录因子, 在植物生长发育和逆境胁迫应答反应中发挥着重要作用。前期研究表明, TaNAC67参与对多种逆境胁迫的应答, 过量表达能增强拟南芥的抗逆性。为进一步揭示其在调控小麦主要农艺性状发育方面的作用, 本研究以36份普通小麦组成的高多态性群体为材料, 测序分析了TaNAC67-6A、TaNAC67-6B、TaNAC67-6D序列多态性, 发现TaNAC67-6A启动子区-1516 nt有1个A/G转换SNP, 在-873~ -748 nt处有1个126 bp的InDel; TaNAC67-6B启动子区-2014和-1916 nt处各有1个C/T转换SNP; TaNAC67-6D启动子区-1795 nt有1个T/G颠换SNP, 编码区357 nt处有1个C/T转换SNP。根据多态性分别开发了功能分子标记, 扫描由282份普通小麦构成的自然群体, 并将基因型和表型性状检测结果进行关联分析, TaNAC67-6A、TaNAC67-6B的标记与表型性状无显著关联, 而TaNAC67-6D的2个标记SNP-D-1和SNP-D-2分别与小麦穗长和每穗小穗数显著相关。单倍型分析发现, 自然群体中存在3种主要单倍型, 其中Hap-6D-3是增加穗长和每穗小穗数的最优单倍型, 在我国小麦育种历史中受到了正向选择。转基因水稻表型分析发现, TaNAC67过表达能显著增加水稻主穗穗长、穗分枝和穗粒数, 验证了小麦关联分析结果。因此, TaNAC67-6D可用于改良农作物穗部性状, 其分子标记可用于小麦分子标记辅助选择育种。

关键词: 小麦, 转录因子, 单核苷酸多态性, 功能标记, 单倍型, 关联分析

Abstract:

NAC transcription factor is a plant specific superfamily and plays an essential role in regulating plant growth and development and stress response. Our previous research indicated that TaNAC67 involved in the response to various environmental stimuli and its overexpression resulted in enhanced tolerance to multiple abiotic stresses. To probe its roles in plant growth and development, a panel consisted of 36 wheat accessions with high diversity we need for polymorphism assays. The genomic sequences, covering the promoter region and gene coding region, were obtained by Sanger sequencing, and named as TaNAC67-6A, TaNAC67-6B, and TaNAC67-6D according to their genomic origins. For TaNAC67-6A, a SNP (A/G) at -1516 nt and a 126 bp InDel between -873 and -748 nt were identified in the promoter region, and one CAPS and one allele specific marker were developed, respectively. For TaNAC67-6B, two SNPs, at -2014 and -1916 nt were detected, and two dCAPS markers were developed accordingly. For TaNAC67-6D, two SNPs, one at -1795 nt in the promoter region, and the other at 357 nt in the gene coding region were identified, and one CAPS and one dCAPS marker were designed, respectively. To probe the relationship of molecular markers and potential agronomic traits, we introduced a population (PA) with 282 common wheat accessions to perform association assay. No association was identified between markers from TaNAC67-6A and 6B and agronomic traits. However, strong associations were identified between SNP-D-1and spike length (SL), and between SNP-D-2 and the total number of spikelet per spike (TNSS). Haplotype assays showed there were three major haplotypes in PA, i.e. Hap-6D-1, -2, -3. Furthermore, the SL of accessions with Hap-6D-3 was significant longer than that with the other two haplotypes under all 30 environments, and the TNSS was significant larger under 22 environments, thus Hap-6D-3 is an elite haplotype for both SL and TNSS, which was selected positively in the process of wheat breeding in China. Further transgenic experiments revealed that TaNAC67-overexpressing rice lines had lager panicles and more seeds relative to wild type, which is consistent with the association assay results. Therefore, TaNAC67 is a potential candidate gene in spike traits improvement, and TaNAC67-6D might be used in marker assistant molecular breeding in wheat.

Key words: wheat, transcription factor, single nucleotide polymorphism, functional marker, haplotype, association assay

表1

试验中使用的引物"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')		 退火温度
Annealing temperature (℃)		 用途
Purpose
NAC67AsF TGTTCCGTATTCAGTACCGGC 55 基因组特异引物
Genome specific primers
NAC67AsR ACACGTTGACCTTACGAATTACAAG
NAC67BsF TATCTCGTCCACTGTTCTCATCTTC 60
NAC67BsR GACTATTGAGAGGTCAGAAGAAGAATG
NAC67DsF GTTAAAGAGAAAGGGAGGAAGGGA 58
NAC67DsR CGTGAGAGTATTGAGAGCTCAGAAG
NAC67AseqF1 ACGTGTGCCCCTATATTTTTAC 测序引物
Sequencing primers
NAC67AseqF2 GACGGCTGAAACACCTAAAAC
NAC67BseqF1 CCGGGCGCGATGTG
NAC67BseqF2 TTAGACACCTAAAAAGTGTCATCG
NAC67DseqF1 GGCTGACAGTAATGATGTTTAGTG
NAC67DseqF2 GTGCCGTTTGGGTCAGG
NAC67ABDseqR CCCGCGGCGTGAAG
A-1516-F GCCTCCTTGTGACTTTGCGA 65 功能标记特异引物
Specific primers for
functional markers
A-1516-R CGCCGGATAATTACAGGTCAGTT
A-126-F GAAGCCTCATTAGGCCCAATT 65
A-126-R TCGACCGGCCGTAGCCT
B-2014-F GGTCTAAGCGGGGTACCGGACTGCA 61
B-2014-R CCTTCTTTGGTATGCCAGGAACTCA
B-1916-F TTGCACAACATGATCATTGAGAGCTA 61
B-1916-R TCACACATCGCGCCCGGC
D-1795-F AGACGATGTGAAGACGACCAAGA 61
D-1795-R ACTGTGGTTCGTGCAACCCTAG
D357-F CCCATCATCGCCGAGGTCGA 64
D357-R CTTCTTGATCCCGACGGTCCTG

图1

TaNAC67-6A序列多态性(A)及等位特异标记SNP-A-1 (B)和CAPS标记SNP-A-2 (C) 图C: 当-1516 nt基因型为A时不能被酶切, 当基因型为G时能够被酶切。M: 100 bp DNA ladder。"

图2

TaNAC67-6B基因序列多态性(A)以及dCAPS标记SNP-B-1 (B)和SNP-B-2 (C) 图B: 当-2014 nt基因型为C时能被酶切, 当基因型为T时不能被酶切; 图C: 当-1916 nt基因型为T时不能被酶切, 当基因型为C时能被酶切。M: 100 bp DNA ladder。"

图3

TaNAC67-6D序列多态性(A)以及CAPS标记SNP-D-1 (B)和dCAPS标记SNP-D-2 (C) 图B: 当-1795 nt基因型是G时能被酶切, 当基因型为T时不能被酶切; 图C: 当357 nt基因型是T时不能被酶切, 当基因型是C时能被酶切。M: 100 bp DNA ladder。"

表2

TaNAC67-6D标记SNP-D-1、SNP-D-2与农艺性状相关性"

年份
Year		 地点
Site		 环境条件
Environment		 性状Trait (P-value)
SNP-D-1-SL SNP-D-2-TNSS
2010 SY DS 2.43E-04*** n.s.
DS+HS 0.00327*** n.s.
WW 0.00346*** 0.02375*
WW+HS 0.01165* 0.00126***
2011 SY DS+HS 0.00162*** n.s.
DS 3.63E-04*** n.s.
WW+HS 1.72E-05*** n.s.
WW 5.24E-04*** 0.03478*
2012 SY DS+HS 0.00192*** 0.00506**
DS 0.00159*** 0.0313*
WW+HS 0.02646* n.s.
WW 0.00363*** 0.03707*
2013 CP DS 0.00129*** 0.01055*
WW 0.00168*** n.s.
2015 SY DS+HS 2.81E-04*** 0.00336***
DS 0.00121*** 0.01369*
WW+HS 0.00268*** n.s.
WW 0.02357* n.s.
2016 SY DS+HS 0.00895** n.s.
DS 0.00996** 0.01447*
WW+HS 9.65E-05*** 0.00877**
WW 0.00788** 4.15E-04***
CP WW 0.0022*** 0.00386***
DS 0.00598** 0.03743*
2017 SY DS+HS 5.86E-04*** 0.02236*
DS 0.01736* 3.51E-05***
WW+HS 9.82E-04*** 0.0054**
WW 0.00184*** 0.01334*
CP DS 0.00153*** 0.02641*
WW 0.00296*** 0.00599***

图4

30种环境下标记SNP-D-1和SNP-D-2两种基因型的表型比较 30种环境条件下标记SNP-D-1 (A)和SNP-D-2 (B)两种基因型穗长(A)和每穗小穗数(B)比较。*、**、***分别表示在0.05、0.01、0.001概率水平差异显著。"

图5

30种环境条件下TaNAC67-6D三种单倍型表型比较 在30种环境条件下3种单倍型穗长(A)和每穗小穗数(B)比较。不同的小写字母表示0.05水平下差异的显著性。"

图6

TaNAC67-6D单倍型在我国十大麦区的地理分布 TaNAC67-6D三个单倍型在我国十大麦区农家品种(A)和育成品种(B)群体中的分布。I: 北方冬麦区; II: 黄淮冬麦区; III: 长江中下游冬麦区; IV: 西南冬麦区; V: 南方冬麦区; VI: 东北春麦区; VII: 北方春麦区; VIII: 西北春麦区; IX: 青-藏冬春麦区; X: 新疆冬春麦区。"

图7

转基因水稻穗部性状比较 转基因水稻穗部特征(A)及转基因水稻(L1、L2)与野生型(WT)主穗的穗长(B)、穗分枝(C)、千粒重(TGW)(D)、穗粒数(E)和空粒数(F)比较。**表示在0.01概率水平差异显著。"

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