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作物学报 ›› 2019, Vol. 45 ›› Issue (12): 1905-1911.doi: 10.3724/SP.J.1006.2019.91016
• 研究简报 • 上一篇
司文洁1,吴林楠1,郭利建1,周梦蝶1,刘香利1,2,马猛1,2,*(
),赵惠贤1,2,*()
Wen-Jie SI1,Lin-Nan WU1,Li-Jian GUO1,Meng-Die ZHOU1,Xiang-Li LIU1,2,Meng MA1,2,*(),Hui-Xian ZHAO1,2,*()
摘要：
为了开发小麦粒重相关基因TaCYP78A5 (Triticum aestivum Cytochrome P450 78A5)的功能标记, 挖掘与千粒重性状相关的优异等位变异, 本研究通过对30份不同品种小麦TaCYP78A5启动子区测序及比对鉴定, 并根据SNP位点差异开发TaCYP78A5-2A启动子区功能标记CAPS-5Ap。结果表明, 在30份不同小麦品种中TaCYP78A5-2A启动子区域出现5个SNP位点差异, 可将30份不同品种小麦分为TaCYP78A5-2Ap-HapI和TaCYP78A5-2Ap-HapII两种单倍型; 以323份现代育成小麦品种验证发现, TaCYP78A5-2Ap-HapI的分布频率为17.96%, TaCYP78A5-2Ap-HapII的分布频率为82.04%, 表明CAPS-5Ap标记可用于小麦TaCYP78A5-2A启动子序列2种单倍型的鉴定。此外, 关联分析发现, CAPS-5Ap标记与粒重相关, 且TaCYP78A5-2Ap-HapII是提高千粒重的优异单倍型。研究结果为小麦分子标记辅助选择和性状改良提供理论依据。
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