作物学报 ›› 2019, Vol. 45 ›› Issue (12): 1899-1904.doi: 10.3724/SP.J.1006.2019.91017
摘要:
为探究不同提取方法对发芽小麦代谢物提取效果的影响, 本研究建立了基于超高效液相色谱-四级杆飞行时间质谱(UPLC-QTOF/MS)的发芽小麦非靶向代谢组学样品前处理方法和分析方法, 以发芽2 d周麦26籽粒为材料, 设计提取溶剂、提取方式、提取时间3个因素在3个水平上L9(3 4)正交试验, 并通过主成分分析和聚类分析确定提取效果最佳的组合。以80%乙腈(0.1%甲酸)震摇提取30 min可检测出1609个代谢峰, 说明提取溶剂对代谢物提取效果起主要作用。共鉴定出92种小麦代谢物。
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