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作物学报 ›› 2014, Vol. 40 ›› Issue (01): 17-21.doi: 10.3724/SP.J.1006.2014.00017

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦puroindoline b-2基因变异与产量相关性状的分析

陈锋,李向楠,曹莹莹,孙建喜,张福彦,董中东,崔党群*   

  1. 河南农业大学农学院 / 河南省粮食作物协同创新中心, 河南郑州 450002
  • 收稿日期:2013-06-13 修回日期:2013-09-16 出版日期:2014-01-12 网络出版日期:2013-10-22
  • 通讯作者: 崔党群, E-mail: cdq62@sohu.com, Tel: 0371-63558537
  • 基金资助:

    本研究由国家自然科学基金项目(31370031),国家重点基础研究发展计划(973计划)项目(2014CB138105)和教育部新世纪优秀人才项目资助。

Analysis of Association of puroindoline b-2 Alleles with Yield-Related Traits in Bread Wheat

CHEN Feng,LI Xiang-Nan,CAO Ying-Ying,SUN Jian-Xi,ZHANG Fu-Yan,DONG Zhong-Dong,CUI Dang-Qun*   

  1. Agronomy College / Collaborative Innovation Center of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2013-06-13 Revised:2013-09-16 Published:2014-01-12 Published online:2013-10-22
  • Contact: 崔党群, E-mail: cdq62@sohu.com, Tel: 0371-63558537

摘要:

puroindoline b-2 (Pinb-2)是近期发现的一组与puoindoline b基因高度相似的基因。Pinb-2基因在普通小麦B基因组中存在多种变异类型,软质麦中不同变异类型间籽粒硬度存在着显著差异。在此基础上进一步分析普通小麦B基因组中的Pinb-2基因等位变异与产量相关性状以及旗叶大小的关系,并利用重组近交系(F8)群体进行染色体定位。结果表明,4种不同变异类型的材料中,拥有Pinb-2v3b类型的小麦品种千粒重、籽粒直径、穗粒数、穗粒重和单株粒重均最高,拥有Pinb-2v3b类型的小麦品种产量相关性状可能优于其他3种类型。另外,拥有Pinb-2v3a类型的小麦品种旗叶宽度、旗叶长度和旗叶面积均高于其他3种类型。采用SSR标记定位结果表明,Pinb-2v2Pinb-2v3互为一对等位基因,位于7B染色体的长臂上,与标记Barc315连锁。这一结果为进一步研究Pinb-2基因的分子遗传基础和小麦育种提供了依据。

关键词: Bread wheat, puroindoline b-2 genes, Yield-related traits, Flag leaf size, Genetic mapping

Abstract:

puroindoline b-2 (Pinb-2) genes were recently discovered and possess high similarity with puroindoline b gene. We have identified several Pinb-2 alleles in B genome of bread wheat (Triticum aestivum L.) and found association between allelic variation on Pinb-2 and grain texture in soft wheat. In this study, we analyzed the association between the Pinb-2 alleles and yield-related traits and flag leaf size, and mapped the Pinb-2 locus using a recombinant inbred line (F8) population. Among the four Pinb-2 genotypes, Pinb-2v3b genotype possessed the highest 1000-grain weight, grain diameter, grain number per spike, grain weight per spike, and grain weight per plant. Therefore, Pinb-2v3b genotype possibly has more preferable yield-related traits than Pinb-2v2, Pinb-2v3a, and Pinb-2v3c genotypes. Additionally, Pinb-2v3a genotype possessed the highest flag leaf width, length, and area. Genetic mapping indicated that Pinb-2 was located on the long arm of chromosome 7B close to SSR marker Barc315. This result provides basic information for use of puroindoline b-2 genes in molecular genetics research and breeding.

Key words: Bread wheat, puroindoline b-2 genes, Yield-related traits, Flag leaf size, Genetic mapping

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