作物学报 ›› 2014, Vol. 40 ›› Issue (02): 301-312.doi: 10.3724/SP.J.1006.2014.00301
杨东清1,李玉玲2,倪英丽1,3,尹燕枰1,杨卫兵1,崔正勇1,张永太2,马仁元2,王振林1,*
YANG Dong-Qing1,LI Yu-Ling2,NI Ying-Li1,3,YIN Yan-Ping1,YANG Wei-Bing1,CUI Zheng-Yong1,ZHANG Yong-Tai2,MA Ren-Yuan2,WANG Zhen-Lin1,*
摘要:
本文旨在揭示持绿型小麦品种的灌浆特性和籽粒蛋白质含量对外源激素的响应,认识植物激素调控籽粒灌浆和蛋白质合成的作用机制。自盛花期开始,连续4 d喷施10 mg L-1脱落酸(ABA)和6-苄基腺嘌呤(6-BA),持绿型品种汶农6号的灌浆期(t3)、活跃生长期(D)、平均灌浆速率(Gmean)、最大灌浆速率(Gmax)、千粒重(TGW)和产量(GY)均大于非持绿型济麦20,且强势粒的各项灌浆参数均大于弱势粒。喷施两种外源激素显著提高两品种的Gmean、Gmax、TGW和GY,对t3和D的影响存在粒位及品种效应。喷施ABA和6-BA均显著提高花后35 d的籽粒蛋白质含量,显著提高汶农6号强、弱势粒的麦谷蛋白含量,但济麦20强、弱势粒的麦谷蛋白含量显著降低。喷施ABA后,汶农6号强势粒花后7~21 d的玉米素核苷(ZR)含量显著升高;喷施6-BA后,济麦20弱势粒花后14~35 d的ZR含量显著升高,而汶农6号强势粒7~21 d的赤霉素(GA3)含量显著降低,弱势粒花后7~28 d的GA3含量显著提高。喷施外源ABA和6-BA显著提高两品种强势粒花后7~21 d的生长素(IAA)和ABA含量。ABA和6-BA处理显著提高花后7~21 d谷氨酰胺合成酶(GS)活性。可见,喷施外源ABA和6-BA使小麦内源激素水平变化,促进籽粒灌浆,增加粒重和产量,提高籽粒GS活性和蛋白质含量,改变蛋白质组分。
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