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作物学报 ›› 2014, Vol. 40 ›› Issue (02): 301-312.doi: 10.3724/SP.J.1006.2014.00301

• 耕作栽培·生理生化 • 上一篇    下一篇

外源ABA和6-BA对不同持绿型小麦籽粒灌浆进程及蛋白质含量影响

杨东清1,李玉玲2,倪英丽1,3,尹燕枰1,杨卫兵1,崔正勇1,张永太2,马仁元2,王振林1,*   

  1. 1山东农业大学农学院 / 作物生物学国家重点实验室,山东泰安 271018; 2齐河县农业局, 山东齐河 251100; 任城区农业局, 山东济宁 272000
  • 收稿日期:2013-06-04 修回日期:2013-09-06 出版日期:2014-02-12 网络出版日期:2013-11-14
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项经费项目(201203100, 201203029),国家自然科学基金项目(31271661),国家重点基础研究发展计划(973计划)项目(2009CB118602),国家粮食丰产科技工程项目(2012BAD04B05)和山东省现代农业技术体系项目经费资助。

Effects of Exogenous ABA and 6-BA on Protein Content and Grain Filling Process in Different Types of Stay-Green Wheat

YANG Dong-Qing1,LI Yu-Ling2,NI Ying-Li1,3,YIN Yan-Ping1,YANG Wei-Bing1,CUI Zheng-Yong1,ZHANG Yong-Tai2,MA Ren-Yuan2,WANG Zhen-Lin1,*   

  1. 1 College of Agronomy, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China; Agriculture Bureau of Qihe County of Shandong Province, Qihe 251100, China; Agriculture Bureau of Rencheng District of Shandong Province, Jining 272000, China
  • Received:2013-06-04 Revised:2013-09-06 Published:2014-02-12 Published online:2013-11-14

摘要:

本文旨在揭示持绿型小麦品种的灌浆特性和籽粒蛋白质含量对外源激素的响应,认识植物激素调控籽粒灌浆和蛋白质合成的作用机制。自盛花期开始,连续4 d喷施10 mg L-1脱落酸(ABA)6-苄基腺嘌呤(6-BA),持绿型品种汶农6号的灌浆期(t3)、活跃生长期(D)、平均灌浆速率(Gmean)、最大灌浆速率(Gmax)、千粒重(TGW)和产量(GY)均大于非持绿型济麦20,且强势粒的各项灌浆参数均大于弱势粒。喷施两种外源激素显著提高两品种的GmeanGmaxTGWGY,对t3D的影响存在粒位及品种效应。喷施ABA6-BA均显著提高花后35 d的籽粒蛋白质含量,显著提高汶农6号强、弱势粒的麦谷蛋白含量,但济麦20强、弱势粒的麦谷蛋白含量显著降低。喷施ABA后,汶农6号强势粒花后7~21 d的玉米素核苷(ZR)含量显著升高;喷施6-BA后,济麦20弱势粒花后14~35 dZR含量显著升高,而汶农6号强势粒7~21 d的赤霉素(GA3)含量显著降低,弱势粒花后7~28 dGA3含量显著提高。喷施外源ABA6-BA显著提高两品种强势粒花后7~21 d的生长素(IAA)ABA含量。ABA6-BA处理显著提高花后7~21 d谷氨酰胺合成酶(GS)活性。可见,喷施外源ABA6-BA使小麦内源激素水平变化,促进籽粒灌浆,增加粒重和产量,提高籽粒GS活性和蛋白质含量,改变蛋白质组分。

关键词: 持绿型小麦, 激素, 灌浆特性, Richard方程, 蛋白质含量, 谷氨酰胺合成酶

Abstract:

This study aimed to understand the grain filling process and protein biosynthesis in grain of stay-green wheat in response to exogenous hormones. At blooming stage, plants of Wennong 6 (stay-green cultivar) and Jimai 20 (control) were sprayed with 10 mg L-1 abscisic acid (ABA) and 6-benzyladenine (6-BA) for four days.Compared with Jimai 20, Wennong 6 had longer grain-filling duration (t3) and the active growth phase (D), higher mean grain-filling rate (Gmean) and maximum grain-filling rate (Gmax), larger thousand-grain weight (TGW), and greater grain yield (GY). All parameters for grain filling were higher in superior grain than in inferior grain. Gmean, Gmax, TGW, and GY were promoted by ABA or 6-BA treatment in both cultivars. However, the effects of exogenous hormones on t3 and D were interacted with grain position in a panicle and genotype. Exogenous ABA and 6-BA treatment obviously increased protein content at 35 days after anthesis (DAA). The glutenin contents in ABA-treated and 6-BA-treated Wennong 6 significantly increased at 35 DAA; however, those in Jimai 20 decreased at 35 DAA. Exogenous ABA increased endogenous zeatin (ZR) content from 7 DAA to 21 DAA. In Wennong 6, GA3 content in superior grain was significantly decreased from 7 DAA to 21 DAA by 6-BA treatment, however, its content in inferior grain was enhanced. In both cultivars, application of ABA and 6-BA resulted in significant increases of endogenous auxin (IAA) and ABA contents from 7 DAA to 21 DAA, along with significant enhancement of glutaminesynthetase (GS) activity.

Key words: Stay-green wheat, Hormones, Grain filling characteristics, Richard equation, Protein content, Glutamine synthetase

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