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作物学报 ›› 2014, Vol. 40 ›› Issue (06): 994-1001.doi: 10.3724/SP.J.1006.2014.009949
李淑洁1,李静雯1,张正英2,*
LI Shu-Jie1,LI Jing-Wen1,ZHANG Zheng-Ying2,*
摘要：
分别构建CaMV35S启动子驱动的Ta6-SFT组成型植物表达载体和rd29A启动子驱动的逆境诱导型表达载体。利用农杆菌介导法分别导入烟草中，获得转基因株系，Southern杂交和Northern点杂交确定Ta6-SFT整合进转基因株系基因组中，并正常转录。以非转基因株系作对照，对2种转基因烟草株系进行干旱胁迫处理，采用半定量RT-PCR分析Ta6-SFT在转基因株系中的表达，同时测定胁迫0 d和18 d果聚糖含量及部分农艺性状。结果表明，含有rd29A启动子的逆境诱导型株系中Ta6-SFT相对表达量比在含CaMV35S启动子的组成型株系中高，而且积累更多的果聚糖；从株高、1/2株高处茎粗、叶面积数据来看，逆境诱导型转基因株系的生长势优于组成型株系，对干旱表现强的耐性。因此，在转基因植物中逆境诱导型表达Ta6-SFT基因将发挥更好的抗逆功能。
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