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作物学报 ›› 2014, Vol. 40 ›› Issue (06): 984-993.doi: 10.3724/SP.J.1006.2014.00984

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

红粒小麦Tamyb10单倍型检测及其与穗发芽抗性的关系

王根平1,2,**,毕惠惠2,**,孙永伟2,王成社1,*,夏兰琴2,*   

  1. 1西北农林科技大学农学院 / 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100; 2中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2013-11-13 修回日期:2014-03-04 出版日期:2014-06-12 网络出版日期:2014-04-09
  • 通讯作者: 夏兰琴, E-mail: xialanqin@caas.cn; 王成社, E-mail: wangcs2008@126.com
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2009CB118305)资助。

Characterization of Tamyb10 Haplotypes and Their Association with Pre-harvest Sprouting Resistance in a Set of Chinese Red-Grained Wheats

WANG Gen-Ping1,2,**,BI Hui-Hui2,**,SUN Yong-Wei2,WANG Cheng-She1,*,XIA Lan-Qin2,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology in Arid Areas, Shanxi Yangling 712100, China; 2Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-11-13 Revised:2014-03-04 Published:2014-06-12 Published online:2014-04-09
  • Contact: 夏兰琴, E-mail: xialanqin@caas.cn; 王成社, E-mail: wangcs2008@126.com

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摘要：

穗发芽是影响小麦品质和产量的重要因素之一，通常红粒小麦比白粒小麦具有较高的穗发芽抗性。转录因子Tamyb10是R-1基因的强候选基因，其表达与否和表达水平决定小麦籽粒颜色。为阐明Tamyb10单倍型与红粒小麦穗发芽抗性的关系，利用已开发的Tamyb10基因分子标记，检测119份来自不同麦区的红粒小麦材料，发现Tamyb10基因(Tamyb10-A1、Tamyb10-B1和Tamyb10-D1位点)可分成7类单倍型，分别是baa、aba、bba、aab、bab、abb和bbb。Tamyb10-D1对穗发芽抗性影响最大，Tamyb10-B1次之，Tamyb10-A1作用最小。Tamyb10单倍型没有明显的地域分布特点，但在东北春麦区，Tamyb10单倍型bbb与红粒品种的高穗发芽抗性相关。

关键词: 红粒小麦, 穗发芽, Tamyb10单倍型;, 麦区

Abstract:

Pre-harvest sprouting (PHS) is one of important factors affecting yield and quality of wheat (Triticum aestivum L.). Generally, red-grained wheat has higher PHS resistance than white wheat. Transcription factor Tamyb10 is a strong candidate for R-1 gene and the expression of its homologues determines wheat seed coat color. In this study, we attempted to analyze the relationship between Tamyb10 haplotype and PHS resistance using amplification result of Tamyb10 specific markers and germination index (GI) evaluated in two locations over two years. In the 119 red-grain Chinese wheat varieties collected from different wheat growth zones in China, seven Tamyb10 haplotypes (Tamyb10-A1, Tamyb10-B1, and Tamyb10-D1 locus) were detected, namely baa, aba, bba, aab, bab, abb, and bbb. Tamyb10 haplotypes were associated with GI value. Tamyb10-D1 had the greatest effect on PHS resistance, followed by Tamyb10-B1 gene, and Tamyb10-A1 had the least effect. Tamyb10 haplotypes distribution showed no obvious regional characteristic, butTamyb10 haplotype bbb correlated with high PHS resistance in the Northeast Spring Wheat Region in China.

Key words: Red-grained wheat, Pre-harvest sprouting, Tamyb10haplotype, Wheat growth zone in China

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