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作物学报 ›› 2019, Vol. 45 ›› Issue (12): 1832-1840.doi: 10.3724/SP.J.1006.2019.91026
杨芳萍1,2,刘金栋2,3,郭莹1,贾奥琳2,闻伟鄂2,巢凯翔4,伍玲5,岳维云6,董亚超2,夏先春2,*(
)
Fang-Ping YANG1,2,Jin-Dong LIU2,3,Ying GUO1,Ao-Lin JIA2,Wei-E WEN2,Kai-Xiang CHAO4,Ling WU5,Wei-Yun YUE6,Ya-Chao DONG2,Xian-Chun XIA2,*()
摘要：
Holdfast是来自英国的小麦品种, 多年来一直保持良好的条锈病持久抗性。本研究目的是发掘Holdfast的条锈病成株抗性基因及其紧密连锁的分子标记, 为小麦持久抗性品种选育提供材料和方法。利用铭贤169和Holdfast杂交后代重组自交系(recombinant inbred lines, RIL)群体, 于2014—2015和2015—2016年度在甘肃甘谷、甘肃中梁和四川成都进行条锈病成株抗性鉴定, 并统计最大严重度(maximum disease severity, MDS)。基于小麦660K SNP芯片和BSA (bulked segregant analysis)技术初步确定抗病基因所在的染色体后, 将目标区域的SNP标记转化为KASP (Kompetitive allele specific PCR)标记, 检测整个RIL群体, 进行基因型分析。最后进行RIL群体条锈病成株抗性的QTL分析, 在5AL和7AL染色体上发现了2个成株抗性QTL。5A染色体长臂上1个条锈病成株抗性QTL QYr.gaas- 5AL, 在所有环境下均存在, 可解释6.5%~9.3%的表型变异; QYr.gaas-5AL位于标记Ax-109948955和Ax-108798241之间, 连锁距离分别为0.5 cM和1.1 cM。在7A染色体长臂上定位到1个条锈病成株抗性QTL QYr.gaas-7AL, 在2015年和2016年甘谷环境中均稳定存在, 分别解释6.2%和7.3%的表型变异; QYr.gaas-7AL位于标记Ax-110361069和Ax-108759561之间, 连锁距离分别为0.5 cM和0.7 cM。携带QYr.gaas-5AL和QYr.gaas-7AL抗病等位基因家系的MDS显著低于感病等位基因家系的MDS, 表明QYr.gaas-5AL和QYr.gaas-7AL可有效降低条锈病严重度, 可应用于小麦抗条锈育种。
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