欢迎访问作物学报，今天是
作物学报 ›› 2024, Vol. 50 ›› Issue (10): 2643-2653.doi: 10.3724/SP.J.1006.2024.44037
郭娟1(
), 辛旭霞1, 冯智尊1, 曹越1, 王晓丹1, 曹晓宁2, 陈凌2, 乔治军2,*(), 王瑞云1,2,*()
GUO Juan1(), XIN Xu-Xia1, FENG Zhi-Zun1, CAO Yue1, WANG Xiao-Dan1, CAO Xiao-Ning2, SANTRA Dipak K3, CHEN Ling2, QIAO Zhi-Jun2,*(), WANG Rui-Yun1,2,*()
摘要：
为构建糜子(Panicum miliaceum L.) DNA分子身份证, 试验以181份陕西糜子核心种质为材料, 对课题组前期开发的糜子特异性SSR标记进行多次PCR筛选和优化后获取核心引物。基于糜子参考基因组信息, 经过BLAST序列比对后将核心标记进行染色体定位。在SSR引物的5′端标注荧光(FAM/HEX), 根据毛细管电泳检测的片段有无采用“0/1”表示, 利用ID Analysis4.0进行区分, 十进制(0~9)统计扩增片段大小构建材料字符串, 用Pop Gene、Power Marker、MEGA、Structure和NTSYS进行遗传多样性分析。试验结果表明, 7个荧光SSR(RYW3、RYW6、RYW37、RYW40、RYW43、RYW125和RYW146)组合可区分181份材料, 不均匀的分布在5条染色体上, 共检测出77个等位变异, 平均为11; 检出Shannon多样性指数(I)为0.8145 (RYW146)~7.8254 (RYW125), 平均5.9076; 观测杂合度(Ho)为0.2627 (RYW146)~0.9506 (RYW3); 期望观测杂合度(He)为0.3329 (RYW146)~0.8747 (RYW125); Nei’s基因多样性指数(Nei)为0.3315 (RYW146)~0.8722 (RYW125); 多态性信息含量(PIC)为0.5923 (RYW146)~0.9445 (RYW125), 平均为0.8419。基于UPGMA将181份资源划分为3个群组。基于主成分分析将材料分为10个类群, 与地理来源一致。利用在线二维码技术(
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