作物学报 ›› 2024, Vol. 50 ›› Issue (10): 2643-2653.doi: 10.3724/SP.J.1006.2024.44037
郭娟1(), 辛旭霞1, 冯智尊1, 曹越1, 王晓丹1, 曹晓宁2, 陈凌2, 乔治军2,*(), 王瑞云1,2,*()
GUO Juan1(), XIN Xu-Xia1, FENG Zhi-Zun1, CAO Yue1, WANG Xiao-Dan1, CAO Xiao-Ning2, SANTRA Dipak K3, CHEN Ling2, QIAO Zhi-Jun2,*(), WANG Rui-Yun1,2,*()
摘要:
为构建糜子(Panicum miliaceum L.) DNA分子身份证, 试验以181份陕西糜子核心种质为材料, 对课题组前期开发的糜子特异性SSR标记进行多次PCR筛选和优化后获取核心引物。基于糜子参考基因组信息, 经过BLAST序列比对后将核心标记进行染色体定位。在SSR引物的5′端标注荧光(FAM/HEX), 根据毛细管电泳检测的片段有无采用“0/1”表示, 利用ID Analysis4.0进行区分, 十进制(0~9)统计扩增片段大小构建材料字符串, 用Pop Gene、Power Marker、MEGA、Structure和NTSYS进行遗传多样性分析。试验结果表明, 7个荧光SSR(RYW3、RYW6、RYW37、RYW40、RYW43、RYW125和RYW146)组合可区分181份材料, 不均匀的分布在5条染色体上, 共检测出77个等位变异, 平均为11; 检出Shannon多样性指数(I)为0.8145 (RYW146)~7.8254 (RYW125), 平均5.9076; 观测杂合度(Ho)为0.2627 (RYW146)~0.9506 (RYW3); 期望观测杂合度(He)为0.3329 (RYW146)~0.8747 (RYW125); Nei’s基因多样性指数(Nei)为0.3315 (RYW146)~0.8722 (RYW125); 多态性信息含量(PIC)为0.5923 (RYW146)~0.9445 (RYW125), 平均为0.8419。基于UPGMA将181份资源划分为3个群组。基于主成分分析将材料分为10个类群, 与地理来源一致。利用在线二维码技术(
[1] | 苏金花. 再论中国古代的糜子. 中国农史, 2018, 37(5): 9-20. |
Su J H. On prosomillet in ancient China. Agric Hist China, 2018, 37(5): 9-20 (in Chinese with English abstract). | |
[2] | Hunt H V, Rudzinski A, Jiang H E, Wang R Y, Thomas M G, Jones M K. Genetic evidence for a western Chinese origin of broomcorn millet (Panicum miliaceum). Holocene, 2018, 28: 1968-1978. |
[3] | 赵志军. 仰韶文化时期农耕生产的发展和农业社会的建立-鱼化寨遗址浮选结果的分析. 江汉考古, 2017, 37(6): 98-108. |
Zhao Z J. The development of agriculture in the time of Yangshao culture and the establishment of agricultural society: an analysis on the flotation result of Yuhuazhai site. Jianghan Archaeol, 2017, 37(6): 98-108 (in Chinese with English abstract). | |
[4] | 高升, 孙周勇, 邵晶, 卫雪, 赵志军. 陕西榆林寨峁梁遗址浮选结果及分析. 农业考古, 2016, 35(3): 14-19. |
Gao S, Sun Z Y, Shao J, Wei X, Zhao Z J. Flotation results and analysis of Zhaomaoliang Site in Yulin, Shaanxi province. Agric Archaeol, 2016, 35(3): 14-19 (in Chinese). | |
[5] | 生膨菲, 尚雪, 张鹏程. 榆林地区龙山晚期至夏代早期先民的作物选择初探. 考古与文物, 2020, 26(2): 114-121. |
Sheng P F, Shang X, Zhang P C. A preliminary study on crop selection from late Longshan to early Xia Dynasty in Yulin area. Archaeol Cult Relics, 2020, 26(2): 114-121 (in Chinese with English abstract). | |
[6] | 郭小宁. 陕北地区龙山晚期的生业方式: 以木柱柱梁、神圪垯梁遗址的植物、动物遗存为例. 农业考古, 2017, 36(3): 19-23. |
Guo X N. The way of life in the late Longshan period in the northern Shaanxi region: taking the plant and animal remains from the Muzhuzhuliang and Shengedaliang Sites as examples. Agric Archaeol, 2017, (3): 19-23 (in Chinese). | |
[7] | 金丹, 尚雪, 郭小宁. 陕西延安觉德遗址两周时期植物资源利用研究. 第四纪研究, 2023, 43: 1483-1492. |
Jin D, Shang X, Guo X N. A study on the utilization of plant resources during the Zhou Dynasty at Juede Site in Yan'an, Shaanxi Province. Quat Sci, 2023, 43: 1483-1492 (in Chinese with English abstract). | |
[8] | 赵志军. 渭河平原古代农业的发展与变化: 华县东阳遗址出土植物遗存分析. 华夏考古, 2019, 32(5): 70-84. |
Zhao Z J. Development and change of ancient agriculture in Weihe Plain: analysis of plant remains unearthed at Dongyang Site in Huaxian County. Huaxia Archaeol, 2019, 32(5): 70-84 (in Chinese with English abstract). | |
[9] | 赵志军, 徐良高. 周原遗址(王家嘴地点)尝试性浮选的结果及初步分析. 文物, 2004, 50(10): 89-96. |
Zhao Z J, Xu L G. Results and preliminary analysis of trial flotation at Zhouyuan Site (Wangjiazui site). Cult Relics, 2004, 50(10): 89-96 (in Chinese with English abstract). | |
[10] | 田多, 邵晶, 裴学松, 李静波. 陕西泾阳蒋刘遗址炭化植物遗存及相关问题分析. 考古与文物, 2024, 30(1): 121-128. |
Tian D, Shao J, Pei X S, Li J B. Analysis of carbonized plant remains and related problems at Jiangliu Site in Jingyang, Shaanxi Province. Archaeol Cult Relics, 2024, 30(1): 121-128 (in Chinese with English abstract). | |
[11] | 唐丽雅, 韩凯, 马明志, 赵志军. 农作物传播研究: 以陕西汉中龙岗寺遗址新石器时代炭化植物遗存为例. 第四纪研究, 2020, 40: 512-524. |
Tang L Y, Han K, Ma M Z, Zhao Z J. The dispersals of crops:A study on the remains of carbonized plants in the Neolithic age at Longgangsi site in Hanzhong, Shaanxi Province. Quat Sci, 2020, 40: 512-524 (in Chinese with English abstract). | |
[12] |
王纶, 王星玉, 乔治军, 温琪汾, 康国帅. 中国黍稷种质资源收集、保护、创新与共享利用. 植物遗传资源学报, 2015, 16: 422-427.
doi: 10.13430/j.cnki.jpgr.2015.02.033 |
Wang L, Wang X Y, Qiao Z J, Wen Q F, Kang G S. Collection, conservation, enhancement, share and utilization of proso millet germplasm resource in China. J Plant Genet Resour, 2015, 16: 422-427 (in Chinese with English abstract). | |
[13] |
郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建. 作物学报, 2021, 47: 80-93.
doi: 10.3724/SP.J.1006.2021.04022 |
Guo Y C, Zhang L L, Chen S Y, Qi J M, Fang P P, Tao A F, Zhang L M, Zhang L W. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.). Acta Agron Sin, 2021, 47: 80-93 (in Chinese with English abstract). | |
[14] |
陶乃奇, 张斌, 刘信凯, 周和达, 钟乃盛, 严丹峰, 张敏, 高继银, 张文驹. 利用荧光标记SSR鉴别21个茶花新品种. 植物学报, 2019, 54: 37-45.
doi: 10.11983/CBB18019 |
Tao N Q, Zhang B, Liu X K, Zhou H D, Zhong N S, Yan D F, Zhang M, Gao J Y, Zhang W J. Identification of 21 new Camellia hybrid varieties by fluorescence-labelled simple sequence repeat markers. Chin Bull Bot, 2019, 54: 37-45 (in Chinese with English abstract). | |
[15] |
王璐伟, 沈志军, 李贺欢, 潘磊, 牛良, 崔国朝, 曾文芳, 王志强, 鲁振华. 基于SSR荧光标记的79份桃种质遗传多样性分析. 中国农业科学, 2022, 55: 3002-3017.
doi: 10.3864/j.issn.0578-1752.2022.15.011 |
Wang L W, Shen Z J, Li H H, Pan L, Niu L, Cui G C, Zeng W F, Wang Z Q, Lu Z H. Analysis of genetic diversity of 79 cultivars based on SSR fluorescence markers for peach. Sci Agric Sin, 2022, 55: 3002-3017 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2022.15.011 |
|
[16] | 娄永峰, 朱柯帆, 宋晓琛, 冷春晖, 陈兴彬, 肖复明. 基于SSR标记构建江西杉木核心种质及其分子身份证. 林业科学研究, 2023, 36(6): 78-86. |
Lou Y F, Zhu K F, Song X C, Leng C H, Chen X B, Xiao F M. Construction of core collection and DNA fingerprinting of Chinese fir germplasms from Jiangxi based on SSR markers. For Res, 2023, 36(6): 78-86 (in Chinese with English abstract). | |
[17] | 杨梦婷, 黄洲, 干建平, 徐君驰, 庞基良. SSR分子标记的研究进展. 杭州师范大学学报(自然科学版), 2019, 18: 429-436. |
Yang M T, Huang Z, Gan J P, Xu J C, Pang J L. Research progress of SSR molecular markers. J Hangzhou Norm Univ (Nat Sci Edn), 2019, 18: 429-436 (in Chinese with English abstract). | |
[18] | 罗兵, 孙海燕, 徐港明, 扬志刚, 沈宗根, 顾雯雯, 高阳, 郑佳亮. SSR分子标记研究进展. 安徽农业科学, 2013, 41: 5210-5212. |
Luo B, Sun H Y, Xu G M, Yang Z G, Shen Z G, Gu W W, Gao Y, Zheng J L. Research progress of SSR molecular marker. J Anhui Agric Sci, 2013, 41: 5210-5212 (in Chinese with English abstract). | |
[19] | 王秀军, 赵彦贝, 王静, 李子航, 张纪堂, 李庆卫. 基于SSR分子标记的175份蜡梅种质遗传多样性分析和指纹图谱构建. 生物工程学报, 2024, 40: 252-268. |
Wang X J, Zhao Y B, Wang J, Li Z H, Zhang J T, Li Q W. Genetic diversity analysis and fingerprinting of 175 Chimonanthus praecox germplasm based on SSR molecular marker. Chin J Biotechnol, 2018, 40: 252-268 (in Chinese with English abstract). | |
[20] | Li K H, Zhang T Z, Narayanamoorthy S, Jin C, Sui Z Q, Li Z J, Li S G, Wu K, Liu G Q, Corke H. Diversity analysis of starch physicochemical properties in 95 proso millet (Panicum miliaceum L.) accessions. Food Chem, 2020, 324: 126863. |
[21] |
陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证. 作物学报, 2022, 48: 908-919.
doi: 10.3724/SP.J.1006.2022.14034 |
Chen X H, Lin Y X, Wang Q, Ding M, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR. Acta Agron Sin, 2022, 48: 908-919 (in Chinese with English abstract). | |
[22] |
陈小红, 何杰丽, 石甜甜, 邵欢欢, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于转录组测序开发糜子SSR标记. 中国农业科学, 2020, 53: 1940-1949.
doi: 10.3864/j.issn.0578-1752.2020.10.002 |
Chen X H, He J L, Shi T T, Shao H H, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Developing SSR markers of proso millet based on transcriptome sequencing. Sci Agric Sin, 2020, 53: 1940-1949 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2020.10.002 |
|
[23] |
何杰丽, 石甜甜, 陈凌, 王海岗, 高志军, 杨美红, 王瑞云, 乔治军. 糜子EST-SSR分子标记的开发及种质资源遗传多样性分析. 植物学报, 2019, 54: 723-732.
doi: 10.11983/CBB19037 |
He J L, Shi T T, Chen L, Wang H G, Gao Z J, Yang M H, Wang R Y, Qiao Z J. The genetic diversity of common millet (Panicum miliaceum) germplasm resources based on the EST-SSR markers. Chin Bull Bot, 2019, 54: 723-732 (in Chinese with English abstract). | |
[24] |
石甜甜, 何杰丽, 高志军, 陈凌, 王海岗, 乔治军, 王瑞云. 利用EST-SSR评估糜子资源遗传差异. 中国农业科学, 2019, 52: 4100-4109.
doi: 10.3864/j.issn.0578-1752.2019.22.014 |
Shi T T, He J L, Gao Z J, Chen L, Wang H G, Qiao Z J, Wang R Y. Genetic diversity of common millet resources assessed with EST- SSR markers. Sci Agric Sin, 2019, 52: 4100-4109 (in Chinese with English abstract). | |
[25] |
薛延桃, 陆平, 史梦莎, 孙昊月, 刘敏轩, 王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究. 作物学报, 2019, 45: 1511-1521.
doi: 10.3724/SP.J.1006.2019.84174 |
Xue Y T, Lu P, Shi M S, Sun H Y, Liu M X, Wang R Y. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu. Acta Agron Sin, 2019, 45: 1511-1521 (in Chinese with English abstract). | |
[26] |
王瑞云, 季煦, 陆平, 刘敏轩, 许月, 王纶, 王海岗, 乔治军. 利用荧光SSR分析中国糜子遗传多样性. 作物学报, 2017, 43: 530-548.
doi: 10.3724/SP.J.1006.2017.00530 |
Wang R Y, Ji X, Lu P, Liu M X, Xu Y, Wang L, Wang H G, Qiao Z J. Analysis of genetic diversity in common millet (Panicum miliaceum) using fluorescent SSR in China. Acta Agron Sin, 2017, 43: 530-548 (in Chinese with English abstract). | |
[27] |
王瑞云, 刘笑瑜, 王海岗, 陆平, 刘敏轩, 陈凌, 乔治军. 用高基元微卫星标记分析中国糜子遗传多样性. 中国农业科学, 2017, 50 : 3848-3859.
doi: 10.3864/j.issn.0578-1752.2017.20.002 |
Wang R Y, Liu X Y, Wang H G, Lu P, Liu M X, Chen L, Qiao Z J. Evaluation of genetic diversity of common millet (Panicum miliaceum) germplasm available in China using high motif nucleotide repeat SSR markers. Sci Agric Sin, 2017, 50: 3848-3859 (in Chinese with English abstract). | |
[28] |
薛延桃, 陆平, 乔治军, 刘敏轩, 王瑞云. 基于SSR标记的黍稷种质资源遗传多样性及亲缘关系研究. 中国农业科学, 2018, 51: 2846-2859.
doi: 10.3864/j.issn.0578-1752.2018.15.002 |
Xue Y T, Lu P, Qiao Z J, Liu M X, Wang R Y. Genetic diversity and genetic relationship of broomcorn millet (Panicum miliaceum L.) germplasm based on SSR markers. Sci Agric Sin, 2018, 51: 2846-2859 (in Chinese with English abstract). | |
[29] |
连帅, 陆平, 乔治军, 张琦, 张茜, 刘敏轩, 王瑞云. 利用SSR分子标记研究国内外黍稷地方品种和野生资源的遗传多样性. 中国农业科学, 2016, 49: 3264-3275.
doi: 10.3864/j.issn.0578-1752.2016.17.002 |
Lian S, Lu P, Qiao Z J, Zhang Q, Zhang Q, Liu M X, Wang R Y. Genetic diversity in broomcorn millet (Panicum miliaceum L.) from China and abroad by using SSR markers. Sci Agric Sin, 2016, 49: 3264-3275 (in Chinese with English abstract). | |
[30] |
Ma S, Han C, Zhou J, Hu R, Jiang X, Wu F, Tain K, Nie G, Zhang X. Fingerprint identification of white clover cultivars based on SSR molecular markers. Mol Biol Rep, 2020, 47: 8513-8521.
doi: 10.1007/s11033-020-05893-7 pmid: 33040266 |
[31] | Meng Y, Zhao N, Li H, Zhai H, He S, Liu Q. SSR fingerprinting of 203 sweetpotato (Ipomoea batatas (L.) Lam.) varieties. J Integr Agric, 2018, 17: 86-93. |
[32] | 吴仕蔓, 娄兵海, 陈传武, 唐艳, 邓崇岭, 武晓晓. 应用SSR荧光标记法构建22个柚类品种的分子身份证. 果树学报, 2023, 40: 605-614. |
Wu S M, Lou B H, Chen C W, Tang Y, Deng C L, Wu X X. Establishment of molecular identity of 22 pomelo varieties using fluorescent labeled SSR markers. J Fruit Sci, 2023, 40: 605-614 (in Chinese with English abstract). | |
[33] | Duan Y, Liu J, Xu J, Bian C, Duan S, Pang W, Hu J, Li G, Jin L. DNA fingerprinting and genetic diversity analysis with simple sequence repeat markers of 217 potato cultivars (Solanum tuberosum L.) in China. Am J Potato Res, 2019, 96: 21-32. |
[34] | Panigrahi P, Panigrahi K K, Bhattacharya S. SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea). Elec J Plant Breed, 2018, 9: 25-37. |
[35] |
王宇卓, 林元香, 薛亚鹏, 段政勇, 王晓丹, 陈凌, 曹晓宁, 王瑞云, 乔治军. 山西糜子核心种质分子身份证构建. 植物学报, 2023, 58: 159-168.
doi: 10.11983/CBB22188 |
Wang Y Z, Lin Y X, Xue Y P, Duan Z Y, Wang X D, Chen L, Cao X N, Wang R Y, Qiao Z J. Construction of molecular ID card of core germplasm of hog millet (Panicum miliaceum) in Shanxi. Chin Bull Bot, 2023, 58: 159-168 (in Chinese with English abstract). | |
[36] |
Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321-4325.
doi: 10.1093/nar/8.19.4321 pmid: 7433111 |
[37] |
薛亚鹏, 丁艺冰, 王宇卓, 王晓丹, 曹晓宁, Santra Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR构建中国糜子核心种质DNA分子身份证. 中国农业科学, 2023, 56: 2249-2261.
doi: 10.3864/j.issn.0578-1752.2023.12.002 |
Xue Y P, Ding Y B, Wang Y Z, Wang X D, Cao X N, Santra Dipak K, Chen L, Qiao Z J, Wang R Y. Construction of DNA molecular identity card of core germplasm of broomcorn millet in China based on fluorescence SSR. Sci Agric Sin, 2023, 56: 2249-2261 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2023.12.002 |
|
[38] | 何瑞超. 绿豆遗传多样性研究及种子萌发期耐盐性评价. 内蒙古农业大学硕士学位论文, 内蒙古呼和浩特, 2021. |
He R C. Study on Genetic Diversity of Mung Bean and Evaluation of Salt Tolerance During Seed Germination. MS Thesis of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China, 2021 (in Chinese with English abstract). | |
[39] | 王瑞云. 糜子遗传多样性及进化研究进展. 北京: 中国农业出版社, 2017. pp 20-92. |
Wang R Y. Research Progress on Genetic Diversity and Evolution of Proso Millet. Beijing: China Agriculture Press, 2017. pp 20-92 (in Chinese). | |
[40] |
王舒婷, 何杰丽, 石甜甜, 陈凌, 王海岗, 王瑞云, 乔治军. 利用微卫星标记分析山西糜子的遗传多样性. 植物遗传资源学报, 2019, 20: 69-78.
doi: 10.13430/j.cnki.jpgr.20180718001 |
Wang S T, He J L, Shi T T, Chen L, Wang H G, Wang R Y, Qiao Z J. Genetic diversity analysis of broomcorn millet (Panicum miliaceum L.) of Shanxi province using microsatellite markers. J Plant Genet Resour, 2019, 20: 69-78 (in Chinese with English abstract). | |
[41] |
王倩, 张立媛, 许月, 李海, 刘少雄, 薛亚鹏, 陆平, 王瑞云, 刘敏轩. 黍稷高基元EST-SSR标记开发及200份核心种质资源遗传多样性分析. 作物学报, 2023, 49: 2308-2318.
doi: 10.3724/SP.J.1006.2023.24201 |
Wang Q, Zhang L Y, Xu Y, Li H, Liu S X, Xue Y P, Lu P, Wang R Y, Liu M X. High motif EST-SSR markers development and genetic diversity evaluation for 200 core germplasms in proso millet. Acta Agron Sin, 2023, 49: 2308-2318 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2023.24201 |
|
[42] | Chen J F, Liu Y, Liu M X, Guo W L, Wang Y Q, He Q, Chen W Y, Liao Y, Zhang W, Gao Y Z, Dong K J, Ren R Y, Yang T Y, Zhang L Y, Qi M Y, Li Z G, Zhao M, Wang H G, Wang J J, Qiao Z J, Li H Q, Jiang Y M, Liu G Q, Song X Q, Deng Y R, Li H, Yan F, Dong Y, Li Q Q, Li T, Yang W Y, Cui J H, Wang H R, Zhou Y F, Zhang X M, Jia G Q, Lu P, Zhi H, Tang S, Diao X M. Pangenome analysis reveals genomic variations associated with domestication traits in broomcorn millet. Nat Genet, 2023, 55: 2243-2254. |
[43] |
Shi J P, Ma X X, Zhang J H, Zhou Y S, Liu M X, Huang L L, Sun S L, Zhang X B, Gao X, Zhan W, Li P H, Wang L, Lu P, Zhao H M, Song W B, Lai J S. Chromosome conformation capture resolved near complete genome assembly of broomcorn millet. Nat Commun, 2019, 10: 464.
doi: 10.1038/s41467-018-07876-6 pmid: 30683940 |
[44] |
Zou C S, Li L T, Miki D, Li D L, Tang Q M, Xiao L H, Rajput S, Deng P, Peng L, Jia W, Huang R, Zhang M L, Sun Y D, Hu J M, Fu X, Schnable P S, Chang Y X, Li F, Zhang H, Feng B L, Zhu X G, Liu R Y, Schnable J C, Zhu J K, Zhang H. The genome of broomcorn millet. Nat Commun, 2019, 10: 436.
doi: 10.1038/s41467-019-08409-5 pmid: 30683860 |
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