欢迎访问作物学报,今天是

作物学报 ›› 2024, Vol. 50 ›› Issue (10): 2643-2653.doi: 10.3724/SP.J.1006.2024.44037

• 研究简报 • 上一篇    下一篇

基于SSR的陕西糜子种质资源的分子鉴定

郭娟1(), 辛旭霞1, 冯智尊1, 曹越1, 王晓丹1, 曹晓宁2, 陈凌2, 乔治军2,*(), 王瑞云1,2,*()   

  1. 1山西农业大学农学院, 中国山西太谷 030801
    2山西农业大学农业基因资源研究中心 / 农业农村部黄土高原作物基因资源与种质创制重点实验室 / 杂粮种质资源发掘与遗传改良山西省重点实验室, 中国山西太原 030031
    3内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心, 美国内布拉斯加州斯克茨布拉夫 69361
  • 收稿日期:2024-03-03 接受日期:2024-06-20 出版日期:2024-10-12 网络出版日期:2024-07-11
  • 通讯作者: *王瑞云, E-mail: wry925@126.com;乔治军, E-mail: nkypzs@126.com
  • 作者简介:E-mail: 1281075333@qq.com
  • 基金资助:
    国家农作物种质资源平台项目(NCGRC-2024-026);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06-14.5-A16);山西省现代农业产业技术体系建设(杂粮)项目(2024CYJSTX0312);山西省重点研发项目(2022ZDYF110);山西农业大学农学院研究生教育改革与质量提升工程项目(2023YCX33);山西农业大学农学院研究生教育改革与质量提升工程项目(2023YCX48);山西农业大学农学院研究生教育改革与质量提升工程项目(2023YCX51);山西农业大学农学院研究生教育改革与质量提升工程项目(2023YDT05)

Molecular identification of broomcorn millet germplasm resources in Shaanxi based on SSR

GUO Juan1(), XIN Xu-Xia1, FENG Zhi-Zun1, CAO Yue1, WANG Xiao-Dan1, CAO Xiao-Ning2, SANTRA Dipak K3, CHEN Ling2, QIAO Zhi-Jun2,*(), WANG Rui-Yun1,2,*()   

  1. 1Agronomy College, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2Center for Agricultural Genetic Resources Research, Shanxi Agricultural University / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs / Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, Shanxi, China
    3Panhandle Research & Extension Center, Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Scottsbluff 69361, Nebraska, USA
  • Received:2024-03-03 Accepted:2024-06-20 Published:2024-10-12 Published online:2024-07-11
  • Contact: *E-mail: wry925@126.com;E-mail: nkypzs@126.com
  • Supported by:
    National crop germplasm resources platform project(NCGRC-2024-026);China Agriculture Research System of MOF and MARA(CARS-06-14.5-A16);Shanxi Province Modern Agricultural Industrial Technology System Construction (Coarse grain) Project(2024CYJSTX0312);Key Research and Development Project of Shanxi Province(2022ZDYF110);Graduate Education Reform and Quality Improvement Project of College of Agronomy, Shanxi Agricultural University(2023YCX33);Graduate Education Reform and Quality Improvement Project of College of Agronomy, Shanxi Agricultural University(2023YCX48);Graduate Education Reform and Quality Improvement Project of College of Agronomy, Shanxi Agricultural University(2023YCX51);Graduate Education Reform and Quality Improvement Project of College of Agronomy, Shanxi Agricultural University(2023YDT05)

摘要:

为构建糜子(Panicum miliaceum L.) DNA分子身份证, 试验以181份陕西糜子核心种质为材料, 对课题组前期开发的糜子特异性SSR标记进行多次PCR筛选和优化后获取核心引物。基于糜子参考基因组信息, 经过BLAST序列比对后将核心标记进行染色体定位。在SSR引物的5′端标注荧光(FAM/HEX), 根据毛细管电泳检测的片段有无采用“0/1”表示, 利用ID Analysis4.0进行区分, 十进制(0~9)统计扩增片段大小构建材料字符串, 用Pop Gene、Power Marker、MEGA、Structure和NTSYS进行遗传多样性分析。试验结果表明, 7个荧光SSR(RYW3、RYW6、RYW37、RYW40、RYW43、RYW125和RYW146)组合可区分181份材料, 不均匀的分布在5条染色体上, 共检测出77个等位变异, 平均为11; 检出Shannon多样性指数(I)为0.8145 (RYW146)~7.8254 (RYW125), 平均5.9076; 观测杂合度(Ho)为0.2627 (RYW146)~0.9506 (RYW3); 期望观测杂合度(He)为0.3329 (RYW146)~0.8747 (RYW125); Nei’s基因多样性指数(Nei)为0.3315 (RYW146)~0.8722 (RYW125); 多态性信息含量(PIC)为0.5923 (RYW146)~0.9445 (RYW125), 平均为0.8419。基于UPGMA将181份资源划分为3个群组。基于主成分分析将材料分为10个类群, 与地理来源一致。利用在线二维码技术(https://cli.im/)构建181份陕西糜子核心种质的DNA分子身份证。

关键词: 糜子, 陕西, 荧光SSR, 毛细管电泳, DNA分子身份证

Abstract:

To construct the DNA molecular identity card of broomcorn millet (Panicum miliaceum L.), we conducted an experiment using 181 core germplasm samples of Shaanxi millets. The core primers were obtained through several rounds of PCR screening and optimization of millet-specific SSR markers developed in the preliminary stage of our research group. Chromosomal localization of the core markers was performed by comparing their sequences with the reference genome information of broomcorn millet using BLAST. FAM/HEX labels were added the 5' end of SSR primers, and the presence or absence of fragments detected by capillary electrophoresis was represented as “0/1”. The differentiation of fragments was carried out using ID Analysis 4.0, and the size of the amplified fragments was recorded in format (0-9) to construct unique material strings. These strings were then subjected to genetic diversity analysis using software tools such as Pop Gene, Power Marker, MEGA, Structure, and NTSYS. Our test results showed that seven combinations of fluorescent SSRs (RYW3, RYW6, RYW37, RYW40, RYW43, RYW125, and RYW146) could distinguish the 181 materials. These markers were unevenly distributed across five chromosomes, and a total of 77 allelic variants were detected, with an average of 11 variants per marker. The observed Shannon's diversity index (I) ranged from 0.8145 (RYW146) to 7.8254 (RYW125), with an average of 5.9076. The observed heterozygosity (Ho) ranged from 0.2627 (RYW146) to 0.9506 (RYW3), while the expected heterozygosity (He) ranged from 0.3329 (RYW146) to 0.8747 (RYW125). Nei’s gene diversity index (Nei) varied from 0.3315 (RYW146) to 0.8722 (RYW125). The Polymorphism Information Content (PIC) ranged from 0.5923 (RYW146) to 0.9445 (RYW125), with an average of 0.8419. Using the UPGMA clustering method, we categorized the 181 resources into three clusters. Principal component analysis resulted in the classification of the materials into ten taxa, which were consistent with their geographic sources. To provide convenient access to the DNA molecular identity cards, we utilized online QR code technology (https://cli.im/) for the 181 Shaanxi broomcorn millet core germplasm samples.

Key words: broomcorn millet, Shaanxi province, fluorescent SSR, capillary electrophoresis, DNA molecular identity card

附表1

参试糜子材料的品种及来源"

序号
Number
统一编号
Unicode
材料名称
Material name
材料来源
Material source
1 00001587 大红黍 Dahongshu 府谷 Fugu
2 00001596 紫秆白黍 Ziganbaishu 府谷 Fugu
3 00001601 紫秆红黍 Ziganhongshu 神木 Shenmu
4 00001604 慢慢黄糜 Manmanhuangmi 神木 Shenmu
5 00001616 牛卵蛋红黍Niuluandanhongshu 榆林 Yulin
6 00001623 花脸软糜 Hualianruanmi 榆林 Yulin
7 00001624 活剥皮糜 Huoopimi 榆林 Yulin
8 00001639 活剥皮 Huobopi 衡山 Hengshan
9 00001641 半点红软糜 Bandianhongruanmi 衡山 Hengshan
10 00001659 焦嘴软糜 Jiaozuiruanmi 靖边 Jingbian
11 00001661 黑疙蚤软糜 Heigezaoruanmi 靖边 Jingbian
12 00001669 信侯头 Xinhoutou 定边 Dingbian
13 00001691 大瓦灰糜Dawahuimi 佳县 Jiaxian
14 00001697 驴驮草 Lutuocao 米脂 Mizhi
15 00001712 黑疙瘩 Heigeda 绥德 Suide
16 00001715 大瓦灰 Dawahui 绥德 Suide
17 00001721 回茬软糜Huicharuanmi 子州 Zizhou
18 00001724 白散散糜 Baisansanmi 子州 Zizhou
19 00001727 狗蝇蛋 Gouyingdan 子州 Zizhou
20 00001731 瓦灰软糜 Wahuiruanmi 子州 Zizhou
21 00001737 黑咀软糜 Heijuruanmi 吴堡 Wubao
22 00001738 白软糜 Bairuanmi 吴堡 Wubao
23 00001748 焦稍白软糜 Jiaoshaobairuanmi 清涧 Qingjian
24 00001756 圪堵黄软糜 Geduhuangruanmi 子长 Zichang
25 00001760 焦咀软糜 Jiaojuruanmi 子长 Zichang
26 00001771 八米二糠 Bamierkang 安塞 Ansai
27 00001775 二瓦灰糜 Erwahuimi 安塞 Ansai
28 00001776 驴驮草 Lutuocao 安塞 Ansai
29 00001781 紫盖头黄糜 Zigaitouhuangmi 志丹 Zhidan
30 00001787 瓦灰软糜 Wahuiruanmi 志丹 Zhidan
31 00001790 黄软糜 Huangruanmi 吴旗 Wuqi
32 00001799 百老软糜Bailaoruanmi 延川 Yanchuan
33 00001800 圪塔糜 Gedami 延川 Yanchuan
34 00001807 草糜子 Caomizi 延长 Yanchang
35 00001815 扫帚黄软糜 Saozhouhuangruanmi 延安 Yanan
36 00001821 紫盖头软糜 Zigaitouruanmi 延安 Yanan
37 00001835 大瓦灰 Dawahui 甘泉 Ganquan
38 00001841 扫帚糜 Saozhoumi 宜川 Yichuan
39 00001848 红软糜 Hongruanmi 富县 Fuxian
40 00001852 洼黑红软糜 Waheihongruanmi 洛川 Luochuan
41 00001854 鹰爪软糜 Yingzhuaruanmi 洛川 Luochuan
42 00001859 爪爪糜 Zhuazhuami 黄陵 Huangling
43 00001864 白软糜 Bairuanmi 黄龙Huanglong
44 00001867 黄糜子Huangmizi 宜君Yijun
45 00001870 黄软糜 Huangruanmi 韩城Hancheng
46 00001871 鸽子蛋 Gezidan 韩城Hancheng
47 00001872 红软糜 Hongruanmi 合阳Heyang
48 00001873 红称锤糜Hongchenchuimi 澄城Chengcheng
49 00001882 黄软糜 Huangruanmi 白水Baishui
50 00001890 羊眼睛糜 Yangyanjingmi 蒲城Pucheng
51 00001891 白软糜 Bairuanmi 蒲城Pucheng
52 00001894 长芒黄糜 Changmanghuangmi 旭县Xuxian
53 00001895 软糜 Ruanmi 旭县Xuxian
54 00001896 红活鼠吊 Honghuoshudiao 大荔Dali
55 00001902 黑糜子 Heimizi 大荔Dali
56 00001903 黑糜子 Heimizi 华阴Huayin
57 00001904 红软糜 Hongruanmi 华县Huaxian
58 00001905 粘糜Nianmi 华县Huaxian
59 00001906 白软糜Bairuanmi 华县Huaxian
60 00001908 白鹑鸽蛋 Baichungedan 渭南Weinan
61 00001909 黑糜子 Heimizi 潼关Tongguan
62 00001910 软糜子Ruanmizi 临潼Lintong
63 00001911 褐红粘糜Hehongnianmi 兰田Lantian
64 00001913 粘糜 Nianmi 铜川Tongchuan
65 00001915 竹叶青 Zhuyeqing 长武Changwu
66 00001918 白鸡蛋 Baijidan 长武Changwu
67 00001922 红老哇头 Honglaowatou 旬邑Xunyi
68 00001930 糯糜子 Nuomizi 彬县Binxian
69 00001934 黑糜子 Heimizi 永寿Yongshou
70 00001935 黄糜子 Huangmizi 户县Huxian
71 00001936 粘糜Nianmi 长安Changan
72 00001938 灰麻糜 Huimami 陇县Longxian
73 00001939 黑糜子Heimizi 千阳Qianyang
74 00001940 红粘糜 Hongnianmi 麟游Linyou
75 00001942 白散芒糜 Baisanmangmi 岐山Qishan
76 00001944 黑糜子 Heimizi 岐山Qishan
77 00001946 白糜子Baimizi 宝鸡Baoji
78 00001949 粘糜 Nianmi 宁陕Ningxia
79 00001950 黄糜子Huangmizi 石泉Shiquan
80 00001951 黄黍子 Huangshuzi 平利Pingli
81 00001952 糜子 Mizi 镇平Zhenpin
82 00001954 黄糜子 Huangmizi 洛南Luonan
83 00001956 黑糜子 Heimizi 洛南Luonan
84 00001957 红糜Hongmi 商县Shangxian
85 00001959 白糜Baimi 商县Shangxian
86 00001963 黄糜 Huangmi 丹凤Danfeng
87 00001964 白糜 Baimi 丹凤Danfeng
88 00001965 糜子 MIzi 丹凤Danfeng
89 00001966 黑糜子 Heimizi 丹凤Danfeng
90 00001967 回茬黄糜 Huichahuangmi 商南Shangnan
91 00001970 软糜子 Ruanmizi 商南Shangnan
92 00001972 黑糜子 Heimizi 商南Shangnan
93 00001973 糜子 Mizi 山阴Shanyin
94 00001974 酒糜 Jiumi 山阴Shanyin
95 00001975 白糜子 Baimizi 山阴Shanyin
96 00001976 黄糜子 Huangmizi 镇安Zhenan
97 00003573 紫秆小黄糜Ziganxiaohuangmi 府谷Fugu
98 00003588 公鸡头小红糜 Gongjitouxiaohongmi 神木Shenmu
99 00003621 白竹糜 Baizhumi 榆林Yulin
100 00003631 牛卵蛋糜 Niuluandanmi 横山Hengshan
101 00003639 灰皮硬糜 Huipiyingmi 横山Hengshan
102 00003642 回茬红小糜 Huichahongxiaomi 靖边Jingbian
103 00003661 小明黑硬糜 Xiaomingheiyingmi 靖边Jingbian
104 00003668 小明红糜 Xiaominghongmi 定边Dingbian
105 00003702 黄糜子 Huangmizi 佳县Jiaxian
106 00003709 红4硬糜 Hong4yingmi 米脂Mizhi
107 00003710 黄糜子 Huangmizi 米脂Mizhi
108 00003712 小日月糜 Xiaoriyuemi 米脂Mizhi
109 00003831 焦咀糜 Jiaojumi 延川Yanchuan
110 00003878 牛尾黄 Niuweihuang 甘泉Ganquan
111 00003964 麦糜 Maimi 澄城Chengcheng
112 00004652 牛蛋黍子 Niudanshuzi 神木Shenmu
113 00004662 公鸡头红软糜(黍) Gongjitouhongruanmi(shu) 榆林Yulin
114 00004671 软竹糜(黍) Ruanzhumi(shu) 横山Hengshan
115 00004692 黑软糜(黍)Heiruanmi(shu) 佳县Jiaxian
116 00004701 黑圪蚤软糜(黍)Heigezaoruanmi(shu) 子洲Zizhou
117 00004707 牛尾稍 Niuweishao 清涧Qingjian
118 00004713 黄淋糜(黍)Huanglinmi(shu) 清涧Qingjian
119 00004716 白软糜(黍)Bairuanmi(shu) 子长Zichang
120 00004728 红软糜(黍)Hongruanmi(shu) 志丹Zhidan
121 00004732 白软糜(黍)Bairuanmi(shu) 志丹Zhidan
122 00004747 红软糜(黍)Hongruanmi(shu) 吴旗Wuqi
123 00004761 焦顶软糜(黍)Jiaodingruanmi(shu) 延安Yanan
124 00004778 竹软糜(黍)Zhuruanmi(shu) 宜川Yichuan
125 00004794 白鹁鸽蛋软糜(黍)Baibogedanruanmi(shu) 渭南Weinan
126 00004796 灰麻糜(黍)Huimami(shu) 长武Changwu
127 00004799 圪垯红糜(黍)Gedahongmi(shu) 旬邑Xunyi
128 00004803 白笊篱头Baizhaolitou 旬邑Xunyi
129 00004806 红软糜(黍)Hongruanmi(shu) 彬县Binxian
130 00004807 黄糜子(黍)Huangmizi(shu) 永寿Yongshou
131 00004808 黄糜子(黍)Huangmizi(shu) 户县Huxian
132 00004816 白老糯糜(黍)Bailainuomi(shu) 商南Shangnan
133 00004821 糯糜子(黍)Nuomizi(shu) 宁陕Ningxia
134 00005206 黄糜子Huangmizi 神木Shenmu
135 00005216 二黄糜Erhuangmi 榆林Yulin
136 00005281 小日月黄糜Xiaoriyuehuangmi 定边Dingbian
137 00005303 灰麻糜Huimami 定边Dingbian
138 00005324 紫秆糜Ziganmi 绥德Suide
139 00005335 牛油黄糜Niuyouhuangmi 子洲Zizhou
140 00005345 黄糜子Huangmizi 吴堡Wubao
141 00005347 白糜Baimi 清涧Qinjian
142 00005373 牛尾黄糜Niuweihuangmi 志丹Zhidan
143 00005387 紫盖头Zigaitou 吴旗Wuqi
144 00005391 黄腰鼠糜Huangyaoshumi 延川Yanchuan
145 00005392 金黄硬糜jinhuangyingmi 延川Yanchuan
146 00005397 紫秆黄糜Ziganhuangmi 延长Yanchang
147 00005424 白落散Bailuosan 延安Yanan
148 00005431 牛尾糜Niuweimi 甘泉Ganquan
149 00005436 白杆老糜Baiganlaomi 宜川Yichuan
150 00005440 黑硬糜Heiyingmi 富县Fuxian
151 00005451 硬糜子Yingmizi 黄陵Huangli
152 00005458 黑糜子Heimizi 宜君Yijun
153 00005462 扫帚糜Saozhoumi 合阳Heyang
154 00005466 称锤黄糜chengchuihuangmi 澄城Chengcheng
155 00005472 枭头糜Xiaotoumi 旭县Xuxian
156 00005476 扎芝糜Zhazhimi 旭县Xuxian
157 00005479 硬糜子Yingmizi 兰田Lantian
158 00005481 鹁鸽蛋Bogedan 铜川Tongchuan
159 00005486 黄糜子 Huangmi 长武Changwu
160 00005492 黄老哇糜 Huanglaowami 旬邑Xunyi
161 00005494 六十天糜 Liushitianmi 彬县Binxian
162 00005496 黄硬糜Huangyingmi 永寿Yongshao
163 00005500 娃娃手Wawashou 乾县Qianxian
164 00005502 圪垯红糜Gedahongmi 陇县Longxian
165 00005506 圪垯糜Gedami 陇县Longxian
166 00005509 杏猴头Xinghoutou 麟游Linyou
167 00005510 黄硬糜Huangyingmi 岐山Qishan
168 00005511 黄糜子Huangmizi 岐山Qishan
169 00005513 平平糜Pinpinmi 扶风Fufeng
170 00005514 牛尾糜Niuweimi 扶风Fufeng
171 00005517 黄手手糜Huangshoushoumi 武功Wugong
172 00005518 牛尾巴Niuweiba 武功Wugong
173 00005520 回茬黄糜Huichahuangmi 商县Shangxian
174 00007556 A75-40 陕西Shanxi
175 00007563 B75-7 陕西Shanxi
176 00007615 F75-1 陕西Shanxi
177 00007896 E75-37 陕西Shanxi
178 00007906 G75-14 陕西Shanxi
179 00008009 B85-108 陕西Shanxi
180 00008022 晋大黄黍子Jindahuangshuzi 陕西榆林Shanxiyulin
181 00008101 晋大01-20-3 Jinda01-20-3 陕西榆林Shanxiyulin

表1

10份糜子试验材料明细表"

材料编号
Serial number
统一编号
Unicode
名称
Name
来源
Source
1 00001623 花脸软糜Hualianruanmi 榆林Yulin
2 00001815 扫帚黄软糜Saozhouhuangruanmi 延安Yan’an
3 00001913 粘糜Nianmi 铜川Tongchuan
4 00001908 白鹑鸽蛋Baichungedan 渭南Weinan
5 00004803 白笊篱头Baizhaolitou 咸阳旬邑Xunyi, Xianyang
6 00005479 硬糜子Yingmizi 西安蓝田Lantian, Xi’an
7 00001967 回茬黄糜Huichahuangmi 商洛商南Shangnan, Shangluo
8 00001946 白糜子Baimizi 宝鸡Baoji
9 00001951 黄黍子Huangshuzi 安康平利Pingli, Ankang
10 00007896 E75-37 陕西Shaanxi

表2

24对荧光SSR引物信息"

引物
Primer name
正向引物序列
Forward sequence (5′-3′)
反向引物序列
Reverse sequence (5′-3′)
退火温度
Tm (℃)
重复碱基
Repeat motif
RYW3 GGAGGCGTGACAATAAAAC GGCGTGAGGTGTTGTTTTT 52.5 (CTGCAA)5
RYW5 GACGATGCTCTTGACCTTGT CACCGTGAAATGTCTCTGCT 55.0 (CCTTT)5
RYW6 AGCCGATTTGCTGTGGAGT CTGCCTCCGATGAGTTGGT 57.4 (ACACC)5
RYW8 GGGTCAGAGAATACACAGCG GTAGGGAAGGAGAAGTGGGT 55.9 (AATAG)5
RYW16 ATCTCCTCCGCCTTCTAACCC TGGCAATGGTCGTACAAACT 56.7 (GAGC)5
RYW17 TCAGCTACTTCGAACGGC GGATCATGCGATACATTTGG 53.0 (TTTC)5
RYW28 CCAAGGCTGAGCAGAAAGAT ACAAGGTGAAACCCGAAGC 55.4 (AGGC)5
RYW29 CTTGATTTCTCACGCACCG TGTCCAGCAGTAGTCGTTCCT 56.2 (GCAG)5
RYW37 CATTCCGTTCCTTGTCTTCC CAGTCTCACTCCTGCGATGT 52.8 (GCGAT)5
RYW40 TGCTCTTCGGCTCTTCTCC ATCAGCTCATCGTGACCCC 57.4 (CAGC)6
RYW42 AGACACCCTGGGCAACATC CTGGACTGGGCTTCGTTCT 57.8 (GGCT)5
RYW43 GGAGATGCTTGCTTGGTTG CAGGAATCGCAAGGAACAG 54.0 (GGAG)5
RYW47 TTGTTTTTGCTGCTGCCTC TGCTGGACTTCTTTTTGCC 53.7 (GCCT)5
RYW50 CAAGGCAGATAGGGCAAGT TCGTCTGCTGCTGGTTTGT 56.1 (GGAG)5
RYW53 ATGCCTCCGATGTAGATGC GCCGCCTTCTCTTCATTCT 54.9 (GAGG)5
RYW55 CTGGTGGTGGTAGTTAGCG TTATGCCACCCACCGTAGC 56.9 (TAGC)5
RYW62 GTTTAGAGAGCAGGAGGCG AGCCCTGTCCACCCTAATC 56.4 (GCTC)5
RYW67 GAAGGAAACGCACCAGAGT TTGGGTTTGTGCTTGGAGT 54.9 (TGCG)5
RYW99 CGGAGTTCTTGGTGGCTT GCGTTCGCCAAAGAGCAT 56.1 (CCA)5
RYW125 TTGACGACGACTGTGTGC TGTTGGTGGAGTTGAGGAC 55.1 (GGC)5
RYW145 CTTTTTCTGCTGCTCCCT TGATGCCATACCCAACTG 52.2 (GAC)6
RYW146 TGATGCTTCTTGGGTTCG CGCCGTCCACTTCTGTAT 53.6 (GCG)6
RYW156 TTTACAACCCTTCCCGCC AGGACTTTCCGCCTCTACCC 57.5 (CCG)5
RYW164 AGACAGCCATTCAACCACG CCATCTCCTCATCCACCA 54.6 (GA)6

表3

7对引物及其组合对糜子种质的区分"

引物/组合
Primer/combination
区分种质数
Number of germplasm distinguished
区分率
Rate of discrimination (%)
RYW3 12 6.63
RYW6 27 14.92
RYW37 0 0
RYW40 11 6.08
RYW43 18 9.94
RYW125 9 4.97
RYW146 5 2.76
RYW3+RYW6 105 59.67
RYW3+RYW6+RYW37 141 77.90
RYW3+RYW6+RYW37+RYW40 165 91.16
RYW3+RYW6+RYW37+RYW40+RYW43 178 98.84
RYW3+RYW6+RYW37+RYW40+RYW43RYW125 179 98.90
RYW3+RYW6+RYW37+RYW40+RYW43RYW125+RYW146 181 100.00

表4

7对核心引物毛细管电泳结果及带型编码"

引物
Primer
荧光基团
Fluorescent group
电泳条带数目
Polymorphism bands
电泳片段长度及编码
Length and code of electrophoresis fragments (bp)
RYW3 FAM (blue) 31 106(01), 106/145/273/408/419(02), 106/145/273/419(03), 106/145/273/419/487(04), 106/145/273/419/495(05), 106/150(06), 106/150/262/273/408/419(07), 106/150/262/273/408/419/487(08), 106/150/262/273/419/439(09), 106/150/262/273/419/439/487(10), 106/150/262/273/419/487(11), 106/150/262/281/408/419(12), 106/150/262/281/408/419/439(13), 106/150/262/281/408/419/478(14), 106/150/262/281/408/419/487(15), 106/150/262/408/419/440/487(16), 106/150/273(17), 106/150/273/281(18), 106/150/273/281/408/419/487(19), 106/150/273/281/408/419(20), 106/150/273/419(21), 106/150/273/419/439(22), 106/150/273/419/439/487(23), 106/150/273/419/487(24), 106/150/273/419/495(25), 106/150/281(26), 106/150/281/408/487(27), 106/150/281/408(28), 106/150/281/487(29), 106/150/487(30), 106/281(31)
RYW6 FAM (blue) 55 110(01), 212(02), 269(03), 282(04), 307(05), 426(06), 110/167/212/269(07), 110/167/269(08), 110/167/269/459(09), 110/188/269(10), 110/188/269/282(11), 110/188/269/373(12), 110/188/269/426(13), 110/188/269/459(14), 110/188/282/426(15), 110/188/307/426(16), 110/196/269/459(17), 110/196/282/426(18), 110/196/307/426(19), 110/196/426(20), 110/212(21), 110/212/269(22), 110/212/269/426(23), 110/212/269/459(24), 110/212/282(25), 110/212/282/426(26), 110/212/307/426(27), 110/212/459(28), 110/269/282(29), 110/282(30), 110/282/426(31), 119/167/269(32), 119/167/269/307(33), 167/212/269(34), 167/212/275(35), 167/275(36), 167/282(37), 188/269/426(38), 188/282(39), 188/282/426(40), 188/307/426(41), 188/373/426(42), 188/426(43), 196/426(44), 212/269(45), 212/269/282(46), 212/282/426(47), 212/307/426(48), 212/426(49), 269/282(50), 269/426(51), 282/426(52), 282/459(53), 307/373/426(54), 307/426(55)
RYW37 FAM (blue) 12 200(01), 205(02), 258(03), 268(04), 200/205(05), 200/210(06), 200/210/268/287/346(07), 200/268/287/346(08), 205/210(09), 205/268/287/346(10), 258/268(11), 268/287/346(12)
RYW40 FAM (blue) 23 153(01), 210(02), 342(03), 381(04), 153/164/326/348/381(05), 153/164/326/381(06), 153/169/197/348/380(07), 153/169/326/348/381(08), 153/188(09), 153/197/326/348/381(10), 153/197/326/379(11), 153/197/348/381(12), 153/210/326/348/381(13), 153/210/326/381(14), 153/210/381(15), 153/210/381/403(16), 153/326/348(17), 153/326/348/381(18), 153/326/348/381/210(19), 153/326/381(20), 153/381(21), 182/326/381(22), 210/381(23)
RYW43 HEX (green) 39 111(01), 136(02), 160(03), 183(04), 304(05), 111/130(06), 111/130/160(07), 111/130/160/166/190/304/323(08), 111/130/160/183(09), 111/130/160/183/304/323(10), 111/130/160/304/323(11), 111/130/160/323(12), 111/130/183(13), 111/130/183/304/323(14), 111/130/183/323(15), 111/130/190/304/323(16), 111/130/304(17), 111/130/304/323(18), 111/130/323(19), 111/160(20), 111/160/304/323(21), 111/160/323(22), 111/323(23), 130/183(24), 130/183/304(25), 130/183/304/480(26), 130/183/323(27), 130/183/323/480(28), 130/183/324(29), 130/183/418/480(30), 130/183/480(31), 136/154/190/304(32), 136/160(33), 136/160/190/253/304(34), 136/160/190/304(35), 136/160/190/304/316(36), 136/160/190/304/323(37), 136/172/190/304/316(38), 160/304(39)
RYW125 FAM (blue) 24 229(01), 106/112/188/241/322(02), 112/118/136(03), 112/118/136/164/241/322(04), 112/118/136/241/322(05), 112/118/164/169/241/322(06), 112/118/164/188/241/322(07), 112/118/164/241/322(08), 112/118/188(09), 112/118/188/241(10), 112/118/188/241/322(11), 112/118/188/322(12), 112/118/241/322(13), 112/127/188/241(14), 112/127/188/241/322(15), 112/164/188/241/322(16), 112/188/241/322(17), 229/241(18), 229/241/251(19), 229/241/251/433(20), 229/241/433(21), 229/251(22), 229/251/433(23), 229/433(24)
RYW146 FAM (blue) 12 196(01), 349(02), 146/166/196/349/427(03), 146/166/196/349/427/451(04), 146/196/252(05), 166/196/349(06), 166/196/349/4279(07), 196/205(08), 196/205/252(09), 196/349(10), 196/349/427(11), 349/427(12)

图1

2个标记在糜子染色体上的位置"

表5

7对荧光 SSR 的遗传多样性参数"

引物
Primer
等位基因数Na 有效等位基因数Ne Shannon
多样性指数I
观测杂合度Ho 期望杂合度
He
Nei’s基因多样性指数Nei’s 多态性信息含量PIC
RYW3 12.0000 6.6660 2.1020 0.9506 0.8526 0.8500 0.9340
RYW6 13.0000 7.3643 2.1711 0.8000 0.8673 0.8642 0.9234
RYW37 7.0000 4.0413 1.6085 0.6885 0.7557 0.7526 0.8014
RYW40 12.0000 6.3446 2.0044 0.8041 0.8467 0.8424 0.7541
RYW43 12.0000 7.6160 2.1649 0.9085 0.8714 0.8687 0.9437
RYW125 13.0000 7.8254 2.1865 0.9492 0.8747 0.8722 0.9445
RYW146 8.0000 1.4958 0.8145 0.2627 0.3329 0.3315 0.5923
平均值Mean 11.0000 5.9076 1.8645 0.7662 0.7716 0.7688 0.8419
标准差SD 2.4495 2.3249 0.5053 0.2415 0.1978 0.1972

图2

基于 UPGMA 的181份糜子种质聚类图 YL: 榆林; YA: 延安; TC: 铜川; WN: 渭南; XY: 咸阳; XA: 西安; SL: 商洛; BJ: 宝鸡; AK: 安康; SX: 陕西。"

图3

基于SSR的181份糜子资源的主成分分析图 YL: 榆林; YA: 延安; TC: 铜川; WN: 渭南; XY: 咸阳; XA: 西安; SL: 商洛; BJ: 宝鸡; AK: 安康; SX: 陕西。"

图4

材料1~6的二维码DNA分子身份证"

附图1

181份糜子的DNA分子身份证"

[1] 苏金花. 再论中国古代的糜子. 中国农史, 2018, 37(5): 9-20.
Su J H. On prosomillet in ancient China. Agric Hist China, 2018, 37(5): 9-20 (in Chinese with English abstract).
[2] Hunt H V, Rudzinski A, Jiang H E, Wang R Y, Thomas M G, Jones M K. Genetic evidence for a western Chinese origin of broomcorn millet (Panicum miliaceum). Holocene, 2018, 28: 1968-1978.
[3] 赵志军. 仰韶文化时期农耕生产的发展和农业社会的建立-鱼化寨遗址浮选结果的分析. 江汉考古, 2017, 37(6): 98-108.
Zhao Z J. The development of agriculture in the time of Yangshao culture and the establishment of agricultural society: an analysis on the flotation result of Yuhuazhai site. Jianghan Archaeol, 2017, 37(6): 98-108 (in Chinese with English abstract).
[4] 高升, 孙周勇, 邵晶, 卫雪, 赵志军. 陕西榆林寨峁梁遗址浮选结果及分析. 农业考古, 2016, 35(3): 14-19.
Gao S, Sun Z Y, Shao J, Wei X, Zhao Z J. Flotation results and analysis of Zhaomaoliang Site in Yulin, Shaanxi province. Agric Archaeol, 2016, 35(3): 14-19 (in Chinese).
[5] 生膨菲, 尚雪, 张鹏程. 榆林地区龙山晚期至夏代早期先民的作物选择初探. 考古与文物, 2020, 26(2): 114-121.
Sheng P F, Shang X, Zhang P C. A preliminary study on crop selection from late Longshan to early Xia Dynasty in Yulin area. Archaeol Cult Relics, 2020, 26(2): 114-121 (in Chinese with English abstract).
[6] 郭小宁. 陕北地区龙山晚期的生业方式: 以木柱柱梁、神圪垯梁遗址的植物、动物遗存为例. 农业考古, 2017, 36(3): 19-23.
Guo X N. The way of life in the late Longshan period in the northern Shaanxi region: taking the plant and animal remains from the Muzhuzhuliang and Shengedaliang Sites as examples. Agric Archaeol, 2017, (3): 19-23 (in Chinese).
[7] 金丹, 尚雪, 郭小宁. 陕西延安觉德遗址两周时期植物资源利用研究. 第四纪研究, 2023, 43: 1483-1492.
Jin D, Shang X, Guo X N. A study on the utilization of plant resources during the Zhou Dynasty at Juede Site in Yan'an, Shaanxi Province. Quat Sci, 2023, 43: 1483-1492 (in Chinese with English abstract).
[8] 赵志军. 渭河平原古代农业的发展与变化: 华县东阳遗址出土植物遗存分析. 华夏考古, 2019, 32(5): 70-84.
Zhao Z J. Development and change of ancient agriculture in Weihe Plain: analysis of plant remains unearthed at Dongyang Site in Huaxian County. Huaxia Archaeol, 2019, 32(5): 70-84 (in Chinese with English abstract).
[9] 赵志军, 徐良高. 周原遗址(王家嘴地点)尝试性浮选的结果及初步分析. 文物, 2004, 50(10): 89-96.
Zhao Z J, Xu L G. Results and preliminary analysis of trial flotation at Zhouyuan Site (Wangjiazui site). Cult Relics, 2004, 50(10): 89-96 (in Chinese with English abstract).
[10] 田多, 邵晶, 裴学松, 李静波. 陕西泾阳蒋刘遗址炭化植物遗存及相关问题分析. 考古与文物, 2024, 30(1): 121-128.
Tian D, Shao J, Pei X S, Li J B. Analysis of carbonized plant remains and related problems at Jiangliu Site in Jingyang, Shaanxi Province. Archaeol Cult Relics, 2024, 30(1): 121-128 (in Chinese with English abstract).
[11] 唐丽雅, 韩凯, 马明志, 赵志军. 农作物传播研究: 以陕西汉中龙岗寺遗址新石器时代炭化植物遗存为例. 第四纪研究, 2020, 40: 512-524.
Tang L Y, Han K, Ma M Z, Zhao Z J. The dispersals of crops:A study on the remains of carbonized plants in the Neolithic age at Longgangsi site in Hanzhong, Shaanxi Province. Quat Sci, 2020, 40: 512-524 (in Chinese with English abstract).
[12] 王纶, 王星玉, 乔治军, 温琪汾, 康国帅. 中国黍稷种质资源收集、保护、创新与共享利用. 植物遗传资源学报, 2015, 16: 422-427.
doi: 10.13430/j.cnki.jpgr.2015.02.033
Wang L, Wang X Y, Qiao Z J, Wen Q F, Kang G S. Collection, conservation, enhancement, share and utilization of proso millet germplasm resource in China. J Plant Genet Resour, 2015, 16: 422-427 (in Chinese with English abstract).
[13] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建. 作物学报, 2021, 47: 80-93.
doi: 10.3724/SP.J.1006.2021.04022
Guo Y C, Zhang L L, Chen S Y, Qi J M, Fang P P, Tao A F, Zhang L M, Zhang L W. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.). Acta Agron Sin, 2021, 47: 80-93 (in Chinese with English abstract).
[14] 陶乃奇, 张斌, 刘信凯, 周和达, 钟乃盛, 严丹峰, 张敏, 高继银, 张文驹. 利用荧光标记SSR鉴别21个茶花新品种. 植物学报, 2019, 54: 37-45.
doi: 10.11983/CBB18019
Tao N Q, Zhang B, Liu X K, Zhou H D, Zhong N S, Yan D F, Zhang M, Gao J Y, Zhang W J. Identification of 21 new Camellia hybrid varieties by fluorescence-labelled simple sequence repeat markers. Chin Bull Bot, 2019, 54: 37-45 (in Chinese with English abstract).
[15] 王璐伟, 沈志军, 李贺欢, 潘磊, 牛良, 崔国朝, 曾文芳, 王志强, 鲁振华. 基于SSR荧光标记的79份桃种质遗传多样性分析. 中国农业科学, 2022, 55: 3002-3017.
doi: 10.3864/j.issn.0578-1752.2022.15.011
Wang L W, Shen Z J, Li H H, Pan L, Niu L, Cui G C, Zeng W F, Wang Z Q, Lu Z H. Analysis of genetic diversity of 79 cultivars based on SSR fluorescence markers for peach. Sci Agric Sin, 2022, 55: 3002-3017 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2022.15.011
[16] 娄永峰, 朱柯帆, 宋晓琛, 冷春晖, 陈兴彬, 肖复明. 基于SSR标记构建江西杉木核心种质及其分子身份证. 林业科学研究, 2023, 36(6): 78-86.
Lou Y F, Zhu K F, Song X C, Leng C H, Chen X B, Xiao F M. Construction of core collection and DNA fingerprinting of Chinese fir germplasms from Jiangxi based on SSR markers. For Res, 2023, 36(6): 78-86 (in Chinese with English abstract).
[17] 杨梦婷, 黄洲, 干建平, 徐君驰, 庞基良. SSR分子标记的研究进展. 杭州师范大学学报(自然科学版), 2019, 18: 429-436.
Yang M T, Huang Z, Gan J P, Xu J C, Pang J L. Research progress of SSR molecular markers. J Hangzhou Norm Univ (Nat Sci Edn), 2019, 18: 429-436 (in Chinese with English abstract).
[18] 罗兵, 孙海燕, 徐港明, 扬志刚, 沈宗根, 顾雯雯, 高阳, 郑佳亮. SSR分子标记研究进展. 安徽农业科学, 2013, 41: 5210-5212.
Luo B, Sun H Y, Xu G M, Yang Z G, Shen Z G, Gu W W, Gao Y, Zheng J L. Research progress of SSR molecular marker. J Anhui Agric Sci, 2013, 41: 5210-5212 (in Chinese with English abstract).
[19] 王秀军, 赵彦贝, 王静, 李子航, 张纪堂, 李庆卫. 基于SSR分子标记的175份蜡梅种质遗传多样性分析和指纹图谱构建. 生物工程学报, 2024, 40: 252-268.
Wang X J, Zhao Y B, Wang J, Li Z H, Zhang J T, Li Q W. Genetic diversity analysis and fingerprinting of 175 Chimonanthus praecox germplasm based on SSR molecular marker. Chin J Biotechnol, 2018, 40: 252-268 (in Chinese with English abstract).
[20] Li K H, Zhang T Z, Narayanamoorthy S, Jin C, Sui Z Q, Li Z J, Li S G, Wu K, Liu G Q, Corke H. Diversity analysis of starch physicochemical properties in 95 proso millet (Panicum miliaceum L.) accessions. Food Chem, 2020, 324: 126863.
[21] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证. 作物学报, 2022, 48: 908-919.
doi: 10.3724/SP.J.1006.2022.14034
Chen X H, Lin Y X, Wang Q, Ding M, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR. Acta Agron Sin, 2022, 48: 908-919 (in Chinese with English abstract).
[22] 陈小红, 何杰丽, 石甜甜, 邵欢欢, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于转录组测序开发糜子SSR标记. 中国农业科学, 2020, 53: 1940-1949.
doi: 10.3864/j.issn.0578-1752.2020.10.002
Chen X H, He J L, Shi T T, Shao H H, Wang H G, Chen L, Gao Z J, Wang R Y, Qiao Z J. Developing SSR markers of proso millet based on transcriptome sequencing. Sci Agric Sin, 2020, 53: 1940-1949 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2020.10.002
[23] 何杰丽, 石甜甜, 陈凌, 王海岗, 高志军, 杨美红, 王瑞云, 乔治军. 糜子EST-SSR分子标记的开发及种质资源遗传多样性分析. 植物学报, 2019, 54: 723-732.
doi: 10.11983/CBB19037
He J L, Shi T T, Chen L, Wang H G, Gao Z J, Yang M H, Wang R Y, Qiao Z J. The genetic diversity of common millet (Panicum miliaceum) germplasm resources based on the EST-SSR markers. Chin Bull Bot, 2019, 54: 723-732 (in Chinese with English abstract).
[24] 石甜甜, 何杰丽, 高志军, 陈凌, 王海岗, 乔治军, 王瑞云. 利用EST-SSR评估糜子资源遗传差异. 中国农业科学, 2019, 52: 4100-4109.
doi: 10.3864/j.issn.0578-1752.2019.22.014
Shi T T, He J L, Gao Z J, Chen L, Wang H G, Qiao Z J, Wang R Y. Genetic diversity of common millet resources assessed with EST- SSR markers. Sci Agric Sin, 2019, 52: 4100-4109 (in Chinese with English abstract).
[25] 薛延桃, 陆平, 史梦莎, 孙昊月, 刘敏轩, 王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究. 作物学报, 2019, 45: 1511-1521.
doi: 10.3724/SP.J.1006.2019.84174
Xue Y T, Lu P, Shi M S, Sun H Y, Liu M X, Wang R Y. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu. Acta Agron Sin, 2019, 45: 1511-1521 (in Chinese with English abstract).
[26] 王瑞云, 季煦, 陆平, 刘敏轩, 许月, 王纶, 王海岗, 乔治军. 利用荧光SSR分析中国糜子遗传多样性. 作物学报, 2017, 43: 530-548.
doi: 10.3724/SP.J.1006.2017.00530
Wang R Y, Ji X, Lu P, Liu M X, Xu Y, Wang L, Wang H G, Qiao Z J. Analysis of genetic diversity in common millet (Panicum miliaceum) using fluorescent SSR in China. Acta Agron Sin, 2017, 43: 530-548 (in Chinese with English abstract).
[27] 王瑞云, 刘笑瑜, 王海岗, 陆平, 刘敏轩, 陈凌, 乔治军. 用高基元微卫星标记分析中国糜子遗传多样性. 中国农业科学, 2017, 50 : 3848-3859.
doi: 10.3864/j.issn.0578-1752.2017.20.002
Wang R Y, Liu X Y, Wang H G, Lu P, Liu M X, Chen L, Qiao Z J. Evaluation of genetic diversity of common millet (Panicum miliaceum) germplasm available in China using high motif nucleotide repeat SSR markers. Sci Agric Sin, 2017, 50: 3848-3859 (in Chinese with English abstract).
[28] 薛延桃, 陆平, 乔治军, 刘敏轩, 王瑞云. 基于SSR标记的黍稷种质资源遗传多样性及亲缘关系研究. 中国农业科学, 2018, 51: 2846-2859.
doi: 10.3864/j.issn.0578-1752.2018.15.002
Xue Y T, Lu P, Qiao Z J, Liu M X, Wang R Y. Genetic diversity and genetic relationship of broomcorn millet (Panicum miliaceum L.) germplasm based on SSR markers. Sci Agric Sin, 2018, 51: 2846-2859 (in Chinese with English abstract).
[29] 连帅, 陆平, 乔治军, 张琦, 张茜, 刘敏轩, 王瑞云. 利用SSR分子标记研究国内外黍稷地方品种和野生资源的遗传多样性. 中国农业科学, 2016, 49: 3264-3275.
doi: 10.3864/j.issn.0578-1752.2016.17.002
Lian S, Lu P, Qiao Z J, Zhang Q, Zhang Q, Liu M X, Wang R Y. Genetic diversity in broomcorn millet (Panicum miliaceum L.) from China and abroad by using SSR markers. Sci Agric Sin, 2016, 49: 3264-3275 (in Chinese with English abstract).
[30] Ma S, Han C, Zhou J, Hu R, Jiang X, Wu F, Tain K, Nie G, Zhang X. Fingerprint identification of white clover cultivars based on SSR molecular markers. Mol Biol Rep, 2020, 47: 8513-8521.
doi: 10.1007/s11033-020-05893-7 pmid: 33040266
[31] Meng Y, Zhao N, Li H, Zhai H, He S, Liu Q. SSR fingerprinting of 203 sweetpotato (Ipomoea batatas (L.) Lam.) varieties. J Integr Agric, 2018, 17: 86-93.
[32] 吴仕蔓, 娄兵海, 陈传武, 唐艳, 邓崇岭, 武晓晓. 应用SSR荧光标记法构建22个柚类品种的分子身份证. 果树学报, 2023, 40: 605-614.
Wu S M, Lou B H, Chen C W, Tang Y, Deng C L, Wu X X. Establishment of molecular identity of 22 pomelo varieties using fluorescent labeled SSR markers. J Fruit Sci, 2023, 40: 605-614 (in Chinese with English abstract).
[33] Duan Y, Liu J, Xu J, Bian C, Duan S, Pang W, Hu J, Li G, Jin L. DNA fingerprinting and genetic diversity analysis with simple sequence repeat markers of 217 potato cultivars (Solanum tuberosum L.) in China. Am J Potato Res, 2019, 96: 21-32.
[34] Panigrahi P, Panigrahi K K, Bhattacharya S. SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea). Elec J Plant Breed, 2018, 9: 25-37.
[35] 王宇卓, 林元香, 薛亚鹏, 段政勇, 王晓丹, 陈凌, 曹晓宁, 王瑞云, 乔治军. 山西糜子核心种质分子身份证构建. 植物学报, 2023, 58: 159-168.
doi: 10.11983/CBB22188
Wang Y Z, Lin Y X, Xue Y P, Duan Z Y, Wang X D, Chen L, Cao X N, Wang R Y, Qiao Z J. Construction of molecular ID card of core germplasm of hog millet (Panicum miliaceum) in Shanxi. Chin Bull Bot, 2023, 58: 159-168 (in Chinese with English abstract).
[36] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321-4325.
doi: 10.1093/nar/8.19.4321 pmid: 7433111
[37] 薛亚鹏, 丁艺冰, 王宇卓, 王晓丹, 曹晓宁, Santra Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR构建中国糜子核心种质DNA分子身份证. 中国农业科学, 2023, 56: 2249-2261.
doi: 10.3864/j.issn.0578-1752.2023.12.002
Xue Y P, Ding Y B, Wang Y Z, Wang X D, Cao X N, Santra Dipak K, Chen L, Qiao Z J, Wang R Y. Construction of DNA molecular identity card of core germplasm of broomcorn millet in China based on fluorescence SSR. Sci Agric Sin, 2023, 56: 2249-2261 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2023.12.002
[38] 何瑞超. 绿豆遗传多样性研究及种子萌发期耐盐性评价. 内蒙古农业大学硕士学位论文, 内蒙古呼和浩特, 2021.
He R C. Study on Genetic Diversity of Mung Bean and Evaluation of Salt Tolerance During Seed Germination. MS Thesis of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China, 2021 (in Chinese with English abstract).
[39] 王瑞云. 糜子遗传多样性及进化研究进展. 北京: 中国农业出版社, 2017. pp 20-92.
Wang R Y. Research Progress on Genetic Diversity and Evolution of Proso Millet. Beijing: China Agriculture Press, 2017. pp 20-92 (in Chinese).
[40] 王舒婷, 何杰丽, 石甜甜, 陈凌, 王海岗, 王瑞云, 乔治军. 利用微卫星标记分析山西糜子的遗传多样性. 植物遗传资源学报, 2019, 20: 69-78.
doi: 10.13430/j.cnki.jpgr.20180718001
Wang S T, He J L, Shi T T, Chen L, Wang H G, Wang R Y, Qiao Z J. Genetic diversity analysis of broomcorn millet (Panicum miliaceum L.) of Shanxi province using microsatellite markers. J Plant Genet Resour, 2019, 20: 69-78 (in Chinese with English abstract).
[41] 王倩, 张立媛, 许月, 李海, 刘少雄, 薛亚鹏, 陆平, 王瑞云, 刘敏轩. 黍稷高基元EST-SSR标记开发及200份核心种质资源遗传多样性分析. 作物学报, 2023, 49: 2308-2318.
doi: 10.3724/SP.J.1006.2023.24201
Wang Q, Zhang L Y, Xu Y, Li H, Liu S X, Xue Y P, Lu P, Wang R Y, Liu M X. High motif EST-SSR markers development and genetic diversity evaluation for 200 core germplasms in proso millet. Acta Agron Sin, 2023, 49: 2308-2318 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2023.24201
[42] Chen J F, Liu Y, Liu M X, Guo W L, Wang Y Q, He Q, Chen W Y, Liao Y, Zhang W, Gao Y Z, Dong K J, Ren R Y, Yang T Y, Zhang L Y, Qi M Y, Li Z G, Zhao M, Wang H G, Wang J J, Qiao Z J, Li H Q, Jiang Y M, Liu G Q, Song X Q, Deng Y R, Li H, Yan F, Dong Y, Li Q Q, Li T, Yang W Y, Cui J H, Wang H R, Zhou Y F, Zhang X M, Jia G Q, Lu P, Zhi H, Tang S, Diao X M. Pangenome analysis reveals genomic variations associated with domestication traits in broomcorn millet. Nat Genet, 2023, 55: 2243-2254.
[43] Shi J P, Ma X X, Zhang J H, Zhou Y S, Liu M X, Huang L L, Sun S L, Zhang X B, Gao X, Zhan W, Li P H, Wang L, Lu P, Zhao H M, Song W B, Lai J S. Chromosome conformation capture resolved near complete genome assembly of broomcorn millet. Nat Commun, 2019, 10: 464.
doi: 10.1038/s41467-018-07876-6 pmid: 30683940
[44] Zou C S, Li L T, Miki D, Li D L, Tang Q M, Xiao L H, Rajput S, Deng P, Peng L, Jia W, Huang R, Zhang M L, Sun Y D, Hu J M, Fu X, Schnable P S, Chang Y X, Li F, Zhang H, Feng B L, Zhu X G, Liu R Y, Schnable J C, Zhu J K, Zhang H. The genome of broomcorn millet. Nat Commun, 2019, 10: 436.
doi: 10.1038/s41467-019-08409-5 pmid: 30683860
[1] 丁艺冰, 辛旭霞, 冯智尊, 郭娟, 曹越, 陈喜明, 王晓丹, 曹晓宁, SANTRA Dipak K, 陈凌, 乔治军, 王瑞云. 东北春播区糜子核心种质的荧光微卫星标记鉴定[J]. 作物学报, 2024, 50(7): 1728-1739.
[2] 丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Dipak K SANTRA, 王瑞云, 陈喜明. 东北春播区糜子核心种质及其DNA分子身份证构建[J]. 作物学报, 2024, 50(5): 1181-1192.
[3] 曹越, 辛旭霞, 冯智尊, 郭娟, 王晓丹, 曹晓宁, SANTRA Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR的宁夏糜子DNA分子身份证的构建[J]. 作物学报, 2024, 50(11): 2699-2711.
[4] 丁敏, 段政勇, 王宇卓, 薛亚鹏, 王海岗, 陈凌, 王瑞云, 乔治军. 糜子GBSSI基因功能标记的开发与验证[J]. 作物学报, 2023, 49(3): 703-718.
[5] 谢呈辉, 马海曌, 许宏伟, 徐郗阳, 阮国兵, 郭峥岩, 宁永培, 冯永忠, 杨改河, 任广鑫. 施氮量对宁夏引黄灌区麦后复种糜子生长、产量及氮素利用的影响[J]. 作物学报, 2022, 48(2): 463-477.
[6] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建[J]. 作物学报, 2021, 47(1): 80-93.
[7] 党科,宫香伟,陈光华,赵冠,刘龙,王洪露,杨璞,冯佰利. 糜子绿豆带状种植下糜子的氮素积累、代谢及产量变化[J]. 作物学报, 2019, 45(12): 1880-1890.
[8] 杨文娟,张艳欣,王林海,魏鑫,黎冬华,高媛,刘盼,张秀荣. 一个芝麻应用核心种质的DNA分子身份证构建[J]. 作物学报, 2018, 44(7): 1010-1020.
[9] 王瑞云,季煦,陆平,刘敏轩,许月,王纶,王海岗,乔治军. 利用荧光SSR分析中国糜子遗传多样性[J]. 作物学报, 2017, 43(04): 530-548.
[10] 周瑜,苏旺,王舰,屈洋,高小丽,杨璞,冯佰利. 不同覆盖方式和施氮量对糜子光合特性及产量性状的影响[J]. 作物学报, 2016, 42(06): 873-885.
[11] 陆徐忠,倪金龙,李莉,汪秀峰,马卉,张小娟,杨剑波. 利用SSR分子指纹和商品信息构建水稻品种身份证[J]. 作物学报, 2014, 40(05): 823-829.
[12] 张仁和,杜伟莉,郭东伟,张爱瑛,胡富亮,李凤艳,薛吉全. 陕西省不同年代玉米品种产量和氮效率性状的变化[J]. 作物学报, 2014, 40(05): 915-923.
[13] 冯晓敏,张永清. 水分胁迫对糜子植株苗期生长和光合特性的影响[J]. 作物学报, 2012, 38(08): 1513-1521.
[14] 朱明, 魏伟, 陈明, 张宪省, 徐兆师, 李连城, 马有志. 一种新的快速鉴定蛋白与靶DNA结合位点的方法[J]. 作物学报, 2011, 37(12): 2167-2172.
[15] 梁强, 张晓科, 尉倩, 王晓龙, 张晶, 孙道杰, 付晓洁. 强筋小麦分子标记多重PCR体系的构建与应用[J]. 作物学报, 2011, 37(11): 1942-1948.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 李余生;朱镇;张亚东;赵凌;王才林. 水稻稻曲病抗性的主基因+多基因混合遗传模型分析[J]. 作物学报, 2008, 34(10): 1728 -1733 .
[2] 赖仲铭; 杨克诚. 全姊妹轮回选择与混合选择对玉米群体改良效果的初步研究[J]. 作物学报, 1983, 9(01): 7 -16 .
[3] 徐孟亮;陈良碧. 4个水稻高产品种与巴西陆稻的耐旱性比较研究[J]. 作物学报, 2003, 29(06): 903 -907 .
[4] 白琪林;陈绍江;戴景瑞. 我国常用玉米自交系秸秆品质性状及其相关分析[J]. 作物学报, 2007, 33(11): 1777 -1781 .
[5] 张洁夫;戚存扣;浦惠明;陈松;陈锋;高建芹;陈新军;顾慧;傅寿仲. 甘蓝型油菜主要脂肪酸组成的QTL定位[J]. 作物学报, 2008, 34(01): 54 -60 .
[6] 戚存扣; 盖钧镒;傅寿仲;浦惠明;张洁夫;高建琴;陈新军. 甘蓝型油菜(Brassica napus L.)千粒重性状遗传体系分析[J]. 作物学报, 2004, 30(12): 1274 -1277 .
[7] 蔡一霞;王维;张祖建;夏广宏;张洪熙;杨建昌;朱庆森. 水旱种植下多个品种蒸煮品质和稻米RVA谱的比较性研究[J]. 作物学报, 2003, 29(04): 508 -513 .
[8] 李木英;石庆华;潘晓华;张荣珍;谭雪明. 两系稻结实期胚乳和茎鞘非结构碳水化合物代谢活性的研究[J]. 作物学报, 2002, 28(06): 821 -828 .
[9] 陈刚;刘巧泉;王忠;顾蕴洁;利佳. Wx蛋白缺失对水稻颖果和剑叶生理活性的影响[J]. 作物学报, 2005, 31(09): 1192 -1197 .
[10] 宋平;季道藩;许复华. 二倍体栽培棉种草棉和亚洲棉的核型比较研究[J]. 作物学报, 1991, 17(02): 102 -106 .