ARC微生物菌剂与复合肥配施对花生光合色素、养分分配及产量的影响

doi:10.3724/SP.J.1006.2026.65017

作物学报 ›› 2026, Vol. 52 ›› Issue (11): 3393-3405.doi: 10.3724/SP.J.1006.2026.65017

ARC微生物菌剂与复合肥配施对花生光合色素、养分分配及产量的影响

张明涛¹，李资文¹，高明萱¹，刘明泽¹，孙雨晗¹，孟添依¹，张鹤¹，王婧¹，蒋春姬¹，刘喜波¹，王晓光¹，张良晓³，李培武³，于海秋²，赵新华^1,*

1 沈阳农业大学农学院, 辽宁沈阳 110866; 2 辽宁农业职业技术学院, 辽宁营口 115009; 3 中国农业科学院油料作物研究所, 湖北武汉 430062

收稿日期:2026-03-08 修回日期:2026-08-21 接受日期:2026-08-21 出版日期:2026-11-12 网络出版日期:2026-08-27
通讯作者: 赵新华, E-mail: Xinhua_zhao@syau.edu.cn
基金资助:
本研究由国家重点研发计划项目“花生、甘薯、马铃薯大面积丰产优质和全程机械化关键技术与集成示范” (2024YFD2301000)和辽宁省科技重大专项——种质创新工程(2025JH1/11700015)资助。

Effects of combined application of ARC microbial agent and chemical fertilizer on photosynthetic pigment contents, nutrient allocation, and peanut yield

Zhang Ming-Tao¹,Li Zi-Wen¹,Gao Ming-Xuan¹,Liu Ming-Ze¹,Sun Yu-Han¹,Meng Tian-Yi¹,Zhang He¹,Wang Jing¹,Jiang Chun-Ji¹,Liu Xi-Bo¹,Wang Xiao-Guang¹,Zhang Liang-Xiao³,Li Pei-Wu³,Yu Hai-Qiu²,Zhao Xin-Hua^1,*

1 College of Agronomy, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; 2 Liaoning Agricultural Vocational and Technical College, Yingkou 115009, Liaoning, China; 3 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, Hubei, China

Received:2026-03-08 Revised:2026-08-21 Accepted:2026-08-21 Published:2026-11-12 Published online:2026-08-27
Supported by:
This study was supported by the National Key R&D Program “Key Technologies and Integrated Demonstration for Large-Scale High-Yield, High-Quality Production and Full-Process Mechanization of Peanuts, Sweet Potatoes, and Potatoes” (2024YFD2301000) and the Liaoning Provincial Major Science and Technology Special Project—Germplasm Innovation Project (2025JH1/11700015).

摘要/Abstract

摘要： 花生(Arachis hypogaea L.)是世界上重要的油料和经济作物。ARC (Aflatoxin Rhizobium Couple)微生物菌剂具有阻控黄曲霉毒素污染与增效豆科作物根瘤固氮的效果。但是，ARC微生物菌剂促进花生减肥、增产的生理机理尚不明确。本试验以花育39为材料，于2023—2024年在沈阳农业大学开展田间定位试验，设置全量施肥(600 kg hm^?2，N-P₂O₅-K₂O = 14-16-15，CK)、全量施肥配施ARC菌剂(600 kg hm^?²+30 kg hm^?²，T0)、减肥10%配施ARC菌剂(540 kg hm^?2+30 kg hm^?2，T1)、减肥25%配施ARC菌剂(450 kg hm^?2+30 kg hm^?2，T2)、减肥40%配施ARC菌剂(360 kg hm^?2+30 kg hm^?2，T3)、减肥55%配施ARC菌剂(270 kg hm^?2+30 kg hm^?2，T4)、不施肥不施菌剂(T5) 7个处理，系统调查了化肥不同施用量与ARC微生物菌剂配施对花生单株生物量积累、光合色素含量、养分吸收利用、肥料利用效率及产量的影响。结果表明，适量减施化肥配施ARC微生物菌剂可显著调控花生生长生理特性，优化植株物质生产与养分利用效率。开花下针期，T1处理下叶片叶绿素a和叶绿素b含量较CK分别显著提高6.49%、8.85%，T1、T2处理下类胡萝卜素含量较CK分别显著提高54.11%、29.74%。结荚期和饱果成熟期，T1处理下叶片谷氨酸合酶(GOGAT)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)及谷氨酸脱氢酶(GDH)活性较CK分别增加12.24%~38.30%、29.78%~94.37%、10.75%~59.37%和34.53%~74.90%。结荚期，T1处理下植株氮、磷、钾积累量较CK分别显著增加4.75%、68.09%、46.87% (P < 0.05)，T2处理下植株磷、钾积累量较CK分别显著增加21.83%、25.05% (P < 0.05)，而T1、T2处理下花生单株生物量较CK分别显著增加47.41%、17.22% (P < 0.05)。适量减施化肥配施ARC微生物菌剂优化植株各器官养分分配格局，促进营养物质向荚果转运与积累。2023和2024年，T0处理农学效率比CK显著提高10.79%~29.52%。产量构成分析表明，与CK相比，T0、T1、T2处理的花生单株荚果数与百果重均显著增加，2024年产量较CK分别显著提升4.88%、12.66%和6.65%。综上所述，适量减肥(450 kg hm^?2~540 kg hm^?2)配施30 kg hm^?² ARC微生物菌剂(T1、T2)可通过改善花生固氮酶活性、促进植株物质积累与氮素代谢利用，协同实现花生产量提升，兼具稳产性与经济高效性。本研究结果可为花生化肥减施、可持续化生产提供理论依据与技术支撑。

关键词: 花生, ARC微生物菌剂, 施肥量, 光合色素, 产量

Abstract:

Peanut (Arachis hypogaea L.) is an important oilseed and cash crop worldwide. The Aflatoxin-Rhizobium Complex (ARC) microbial agent is effective in controlling aflatoxin contamination and enhancing nitrogen fixation via root nodules in legume crops, whereas the physiological mechanism by which the ARC microbial agent increases peanut yield and reduces fertilizer use remains unclear. In this study, ‘Huayu 39’ was used in field trials at Shenyang Agricultural University during the 2023–2024 growing seasons. Seven treatments were set: full fertilization (600 kg hm?², N-P?O?-K?O = 14-16-15, CK), full fertilization combined with the ARC microbial agent (600 kg hm?² + 30 kg hm?², T0), 10% reduction in fertilizer combined with the application of the ARC microbial agent (540 kg hm?² + 30 kg hm?², T1), 25% reduction in fertilizer combined with the application of the ARC microbial agent (450 kg hm?² + 30 kg hm?², T2), 40% reduction in fertilizer combined with the application of the ARC microbial agent (360 kg hm?² + 30 kg hm?², T3), 55% reduction in fertilizer combined with the application of the ARC microbial agent (270 kg hm?² + 30 kg hm?², T4), and no fertilizer or microbial agent applied (T5), to systematically investigate the effects of different chemical fertilizer application rates combined with the ARC microbial agent on biomass accumulation per peanut plant, photosynthetic pigments, nutrient uptake and utilization, fertilizer utilization efficiency, and yield. The results indicated that a moderate reduction in chemical fertilizer application, combined with the application of the ARC microbial agent, significantly modulated the growth and physiological characteristics of peanut plants, optimizing plant biomass production and nutrient use efficiency. During the flowering-pegging stage, the chlorophyll a and chlorophyll b contents in leaves under T1 were significantly higher than those in CK by 6.49% and 8.85%, respectively, whilst the carotenoid content under T1 and T2 was significantly higher than that in CK by 54.11% and 29.74%, respectively (P < 0.05). During the pod-setting and pod-filling stages, the activities of glutamate synthase (GOGAT), nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in leaves under T1 increased by 12.24%–38.80%, 29.78%–94.37%, 10.75%–59.37% and 34.53%–74.90% compared with CK. During the pod-setting stage, under the T1 treatment, the accumulation of nitrogen, phosphorus and potassium in plants increased significantly by 4.75%, 68.09% and 46.87%, respectively, compared with CK (P < 0.05), whilst under T2, phosphorus and potassium accumulation in plants increased significantly by 21.83% and 25.05%, respectively (P < 0.05). Furthermore, the biomass per peanut plant under T1 and T2 increased significantly by 47.41% and 17.22%, respectively, compared with CK (P < 0.05). Moderately reducing chemical fertilizer application and combining this with the application of the ARC microbial agent optimized the pattern of nutrient allocation among plant organs, thereby promoting the transport and accumulation of nutrients in the pods. In 2023 and 2024, the agronomic efficiency of the T0 treatment was significantly higher than that of CK by 10.79%–29.52%. Analysis of yield composition showed that, compared with the control (CK), the number of pods per plant and the weight of 100 pods were both significantly higher in the T0, T1 and T2 treatments; yields in 2024 were significantly higher than those of the control by 4.88%, 12.66% and 6.65%, respectively. In summary, a moderate reduction in chemical fertilizer application (450 kg hm^?2 to 540 kg hm^?2) combined with the application of 30 kg hm^?2 of the ARC microbial agent (T1, T2) synergistically enhanced peanut yield by improving nitrogenase activity, promoting plant biomass accumulation and nitrogen metabolism and utilization, thereby achieving both stable yields and economic efficiency. The findings of this study provide theoretical and technical support for reducing chemical fertilizer application and promoting sustainable peanut production.

Key words: peanut, ARC microbial agent, fertilizer rates, photosynthetic pigment contents, yield

张明涛, 李资文, 高明萱, 刘明泽, 孙雨晗, 孟添依, 张鹤, 王婧, 蒋春姬, 刘喜波, 王晓光, 张良晓, 李培武, 于海秋, 赵新华. ARC微生物菌剂与复合肥配施对花生光合色素、养分分配及产量的影响[J]. 作物学报, 2026, 52(11): 3393-3405.

Zhang Ming-Tao, Li Zi-Wen, Gao Ming-Xuan, Liu Ming-Ze, Sun Yu-Han, Meng Tian-Yi, Zhang He, Wang Jing, Jiang Chun-Ji, Liu Xi-Bo, Wang Xiao-Guang, Zhang Liang-Xiao, Li Pei-Wu, Yu Hai-Qiu, Zhao Xin-Hua. Effects of combined application of ARC microbial agent and chemical fertilizer on photosynthetic pigment contents, nutrient allocation, and peanut yield[J]. Acta Agronomica Sinica, 2026, 52(11): 3393-3405.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2026.65017

https://zwxb.chinacrops.org/CN/Y2026/V52/I11/3393

参考文献

[1] He S N, Chen Y, Xiang W, et al. Carbon and nitrogen footprints accounting of peanut and peanut oil production in China. J Clean Prod, 2021, 291: 125964.

[2] 张萍, 冮东明, 郭佩, 等. 东北花生高产高效结瘤种质筛选及生理特性. 中国油料作物学报, 2025, 47: 1254–1263.

Zhang P, Gang D M, Guo P, et al. Screening and physiological characteristics of peanut germplasm with high yield and high nodulation efficiency in Northeast China. Chin J Oil Crop Sci, 2025, 47: 1254–1263 (in Chinese with English abstract).

[3] Wu Y, Sun Z Q, Qi F Y, et al. Comparative transcriptomics analysis of developing peanut. (Arachis hypogaea L.) pods reveals candidate genes affecting peanut seed size. Front Plant Sci, 2022, 13: 958808.

[4] Liu X J, Hu B, Chu C C. Nitrogen assimilation in plants: current status and future prospects. J Genet Genom, 2022, 49: 394–404.

[5] Albornoz F. Crop responses to nitrogen overfertilization: a review. Sci Hortic, 2016, 205: 79–83.

[6] 周扬, 岳晓凤, 唐晓倩, 等. 黄曲霉毒素阻控与花生超级结瘤耦合效应研究初报. 中国油料作物学报, 2021, 43: 947–960.

Zhou Y, Yue X F, Tang X Q, et al. A preliminary study on the coupling effect of aflatoxin green control and super-nodulation. Chin J Oil Crop Sci, 2021, 43: 947–960 (in Chinese with English abstract).

[7] 郭陞垚, 詹柳琪, 黄佳华, 等. 氮肥减施条件下ARC菌剂对花生产量及品质的影响. 中国油料作物学报, 2025, 47: 1225–1231.

Guo S Y, Zhan L Q, Huang J H, et al. Effect of ARC microbial agent on peanut yield and quality under nitrogen fertilizer reduction conditions. Chin J Oil Crop Sci, 2025, 47: 1225–1231 (in Chinese with English abstract).

[8] 陈丽华, 李晨净, 李倩, 等. 减氮施用ARC微生物菌剂和根瘤菌对大豆产量和品质的影响. 饲料研究, 2026, 49(1): 142–147.

Chen L H, Li C J, Li Q, et al. Effects of nitrogen reduction combined with ARC microbial agent and rhizobia on soybean yield and quality. Feed Res, 2026, 49(1): 142–147 (in Chinese with English abstract).

[9] 吴子佶. ARC微生物菌剂对大豆生长及品质的影响. 武汉轻工大学硕士学位论文, 湖北武汉, 2025.

Wu Z J. Effect of ARC Microbial Agent on the Growth and Quality of Soybean. MS Thesis of Wuhan Polytechnic University, Wuhan, Hubei, China: 2025 (in Chinese with English abstract).

[10] Qiu N W, Wang X S, Zhou F. A new method for fast extraction and determination of chlorophylls in natural water. Z Naturforsch C J Biosci, 2018, 73: 77–86.

[11] 李朝英, 郑路, 卢立华, 等. 测定植物全氮的H₂SO₄-H₂O₂消煮法改进. 中国农学通报, 2014, 30(6): 159–162.

Li Z Y, Zheng L, Lu L H, et al. Improvement in the H₂SO₄-H₂O₂digestion method for determining plant total nitrogen. Chin Agric Sci Bull, 2014, 30(6): 159–162 (in Chinese with English abstract).

[12] Wang H L, Zhang X C, Zhang G P, et al. Regulation of density and fertilization on crude protein synthesis in forage maize in a semiarid rain-fed area. Agriculture, 2023, 13: 715.

[13] Zhao B, Tian M, An Q, et al. Characteristics of a heterotrophic nitrogen removal bacterium and its potential application on treatment of ammonium-rich wastewater. Bioresour Technol, 2017, 226: 46–54.

[14] Ding F, Hu Q N, Wang M L, et al. Knockout of SlSBPASE suppresses carbon assimilation and alters nitrogen metabolism in tomato plants. Int J Mol Sci, 2018, 19: 4046.

[15] 王淑君, 刘金荣, 王素英, 等. 豫谷18功能叶碳氮代谢关键酶活性研究. 中国种业, 2021(6): 59–62.

Wang S J, Liu J R, Wang S Y, et al. Study on key enzyme activities of carbon and nitrogen metabolism in functional leaves of Yugu 18. China Seed Ind, 2021(6): 59–62 (in Chinese with English abstract).

[16] Zhao X, Guo P P, Wu X, et al. Optimizing cotton growth in saline soil: compound microbial agent modulates indigenous bacteria to enhance photosynthesis and vegetative-reproductive balance. Ind Crops Prod, 2024, 221: 119286.

[17] Zhang G G, Yang R N, Ma X D, et al. ARC microbial inoculants promote peanut growth and yield while suppressing Aspergillus flavus infection. Oil Crop Sci, 2025, 10: 109–117.

[18] 赵跃, 吕永超, 陈小姝, 等. 不同施氮水平对黑钙土花生碳氮代谢相关酶活性、产量和品质的影响. 中国油料作物学报, 2024, 46: 122–128.

Zhao Y, Lyu Y C, Chen X S, et al. Effects of nitrogen application rates on enzyme activities related to carbon and nitrogen metabolism, yield and quality of peanut in chernozem. Chin J Oil Crop Sci, 2024, 46: 122–128 (in Chinese with English abstract).

[19] Li R, He Z B, He P, et al. Crop diversification improves productivity and soil health by regulating fungal diversity. Soil Tillage Res, 2025, 252: 106628.

[20] 焦念元, 李吉明, 汪江涛, 等. 氮磷对玉米花生间作蛋白质与氮代谢特点的影响. 作物杂志, 2014(6): 99–105.

Jiao N Y, Li J M, Wang J T, et al. Effects of nitrogen and phosphorus on protein and nitrogen metabolism characteristics in maize peanut intercropping system. Crops, 2014(6): 99–105 (in Chinese with English abstract).

[21] Huang Y X, Tang S Y, Liu R M, et al. Soil organic carbon mediates plant immunity-rhizosphere microbiome interactions and controls colonization resistance to microbial inoculants. Cell Host Microbe, 2025, 33: 1929–1944.e7.

[22] 陈展宇, 房晓琨, 孙帆, 等. 不同年代大豆品种根系氮代谢关键酶活性变化. 吉林农业大学学报, 2024, 46: 366–372.

Chen Z Y, Fang X K, Sun F, et al. Changes in key enzyme activities of root nitrogen metabolism in soybean varieties released at different ages. J Jilin Agric Univ, 2024, 46: 366–372 (in Chinese with English abstract).

[23] 何芬, 赵蕊, 黄弯凤, 等. 芒萁根际复合微生物菌剂对绿豆幼苗生长及根际微生物群落的影响. 微生物学通报, 2025, 52: 1073–1088.

He F, Zhao R, Huang W F, et al. Rhizosphere complex microbial agent of icranopteris dichotoma regulates the growth and rhizosphere microbial community of mung bean seedlings. Microbiol China, 2025, 52: 1073–1088 (in Chinese with English abstract).

[24] 侯睿, 张小红, 张小军, 等. 生物菌肥在花生上施用效果的研究. 中国农学通报, 2017, 33(8): 78–83.

Hou R, Zhang X H, Zhang X J, et al. The application effect of biological bacterial fertilizer on peanut. Chin Agric Sci Bull, 2017, 33(8): 78–83 (in Chinese with English abstract).

[25] 邹晓霞, 张甜, 王丽丽, 等. 黑曲霉菌肥施用对花生碳氮代谢、产量及籽仁品质的影响. 植物生理学报, 2020, 56: 1974–1984.

Zou X X, Zhang T, Wang L L, et al. Effect of Aspergillus niger biofertilizer on carbon and nitrogen metabolism, yield and kernel quality of peanut. Plant Physiol J, 2020, 56: 1974–1984 (in Chinese with English abstract).

[26] Copeland C, Schulze-Lefert P, Ma K W. Potential and challenges for application of microbiomes in agriculture. Plant Cell, 2025, 37: koaf185.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

ARC微生物菌剂与复合肥配施对花生光合色素、养分分配及产量的影响

Effects of combined application of ARC microbial agent and chemical fertilizer on photosynthetic pigment contents, nutrient allocation, and peanut yield

PDF

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	唐海江, 郭富城, 肖东浩, 杨九菊, 苏晶, 廖婷璐, 王艳旗, 罗成科. 外源硝普钠喷施对盐碱地水稻碳氮代谢调控机制及增产效应研究[J]. 作物学报, 2026, 52(9): 2744-2759.
[2]	黄迎, 刘沛琦, 解森, 许洛, 任元, 刘旭洋, 何冠华, 李永祥, 王天宇, 黎裕, 周涛, 廖江林, 李春辉, 张登峰. 玉米氮利用效率全基因组关联分析及候选基因挖掘[J]. 作物学报, 2026, 52(9): 2558-2572.
[3]	潘丽娟, 姜骁, 许静, 殷祥贞, 万菲菲, 孙淑娟, 赵旭红, 陈娜, 马俊卿, 迟晓元. 花生蔗糖含量相关基因的定位及KASP标记的开发与验证[J]. 作物学报, 2026, 52(9): 2598-2607.
[4]	杨璐瑶, 崔茹莹, 董学瑞, 闫鹏, 张娟, 张彩霞, 王永军, 董志强, 卢霖. 聚天冬氨酸钾和甜菜碱复配剂对灌浆期高温胁迫下小麦同化物积累、转运及产量的缓解效应[J]. 作物学报, 2026, 52(9): 2760-2774.
[5]	赵岩, 张文姣, 杨琦文, 魏佳智, 俞晓雪, 李丹丹, 魏楚, 冯誉誉, 彭佳泺, 王彩香, 马雄风, 宿俊吉. 陆地棉产量相关性状QTL的Meta分析及候选基因鉴定[J]. 作物学报, 2026, 52(9): 2584-2597.
[6]	崔雯杰, 闫喆林, 任强, 樊志龙, 殷文, 孙亚丽, 范虹, 何蔚, 王凤, 胡发龙, 柴强. 氮肥后移提高麦玉间作系统玉米产量的光合生理机制[J]. 作物学报, 2026, 52(9): 2700-2712.
[7]	李宗, 叶荣柒, 孙会军, 郭威宏, 罗子恒, 刘硕, 任永峰, 张雯, 宫香伟, 姜英. 间作绿肥对东北春玉米产量及农田土壤质量的影响[J]. 作物学报, 2026, 52(9): 2775-2791.
[8]	王嘉豪, 王小玲, 范震, 郭光旭, 冯文静, 张新博, 罗璇, 任小龙, 陈小莉. 液态地膜与微垄沟种植集成对旱区夏玉米光能利用及产量的提升效应[J]. 作物学报, 2026, 52(9): 2713-2724.
[9]	郎瑞博, 黄莉, 刘念, 晏立英, 陈玉宁, 王欣, 王前前, 康彦平, 王志慧, 姜慧芳, 廖伯寿, 雷永, 淮东欣. 花生含油量分子标记开发及其在高油育种中的应用[J]. 作物学报, 2026, 52(8): 2327-2335.
[10]	许静, 樊博恒, 王恩琪, 潘丽娟, 殷祥贞, 陈娜, 乔利仙, 姜骁, 赵旭红, 马俊卿, 张昆, 刘风珍, 迟晓元. 花生籽仁品质性状与种皮颜色遗传分析及基因定位研究[J]. 作物学报, 2026, 52(8): 2279-2296.
[11]	王碧胜, 孙筱璐, 崔玉莎, 赵中情, 马青敏, 丁鹤洋, 房全孝. 冬小麦产量形成对播种密度及行距的响应模拟改进与验证[J]. 作物学报, 2026, 52(8): 2438-2453.
[12]	高旺南, 孟天琪, 孙兆鑫, 尚旭阁, 武军, 刘玉秀. 小麦茎秆实心性状研究进展及育种应用[J]. 作物学报, 2026, 52(8): 2233-2243.
[13]	黄周详, 李乙弘, 刘笑言, 刘伟, 孟玉山, 唐道彬, 张凯, 杜康, 王季春, 吕长文. 6-BA与烯效唑对前旱后湿胁迫下甘薯光合性能、抗逆生理及产量品质的影响[J]. 作物学报, 2026, 52(8): 2521-2532.
[14]	谢可盈, 李巧龙, 李国风, 赵明慧, 高瑞蔓, 凌英华, 赵芳明. 基于水稻CSSL-Z815构建SSSLs的产量相关QTL鉴定与qGL8精细定位[J]. 作物学报, 2026, 52(8): 2350-2365.
[15]	向钰鑫, 张捷语, 李贵珍, 王琳, 刘涵, 秦宇星, 马根, 向达兵. 氮密互作对苦荞冠层光环境、产量及品质的影响[J]. 作物学报, 2026, 52(8): 2480-2495.