关键词: 小麦（Triticum aestivum）, 铝胁迫, 抑制差减杂交, 诱导基因

Abstract:

Twenty-nine aluminum-induced genes were identified on the base of nylon filter arrays, which contained all clones from a suppression subtractive hybridization (SSH) cDNA library constructed from roots of OK91G106 (the wheat line with high ability of aluminum tolerance). The induced genes included 20 known biological functions and 9 functions unknown by BLAST_n analysis in international biological website NCBⅠ. We found that the induced genes under aluminum stress could be classified into different groups according to the deduced biological function, including signal transduction, active oxygen scavenging, sustaining stability of membrane structure, secretion of malate, and protection of cellular function. It suggested that the wheat plants involved the perception and transduction of the signal of aluminum stress, by which to induce the expression of some genes response to aluminum stress in short time under aluminum stress condition. The results from Northern blot analysis for five induced genes were all similar to those from the nylon filter arrays. There was one malate transporter gene among the 29 aluminum-induced genes, indicating that the increase of malate secretion under aluminum stress condition is possibly an important mechanism for the tested wheat line to response and withstand the aluminum toxicity.

Key words: Wheat (Triticum aestivum L.), Aluminum stress, Suppression subtractive hybridization(SSH), Induced genes