关键词: 核心种质, 栽培大豆, 野生大豆, 28K过敏蛋白, 30K过敏蛋白

Abstract:

Soybean core collection is very important foundation for novel gene mining. Part of soybean core collection was used to compare with reserved germplasm and wild soybean by using immunoblotting, in order to select Gly m Bd 30K and Gly m Bd 28K allergen-null germplasm, and evaluate the representation of core collection. The result showed that all the materials, including 60 wild soybean and 421 cultivars, contained Gly m Bd 30K and the absence ratio of Gly m Bd 28K in cultivars (37.8%) was higher than that in wild soybean (13.3%). The distribution tends of Gly m Bd 28K null materials in core collection was similar to that in reserved collection. The absence ratios of Gly m Bd 28K were not significantly different in three ecotype regions of both core collection and conserved collection, which indicated that the core collection has perfect representative. SSR marker analysis showed that similarity index among 80% cultivars lacking Gly m Bd 28K was less than 0.44, which indicated these germplasms had abundant genetic diversity and could be used in the breeding of Gly m Bd 28K lacking varieties.

Key words: Core collection, Cultivar, Wild Soybean, Gly m Bd 28K, Gly m Bd 30K