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作物学报 ›› 2009, Vol. 35 ›› Issue (5): 761-767.doi: 10.3724/SP.J.1006.2009.00761

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

来自野生二粒小麦IW3和IW10的两个白粉病基因的鉴定及SSR标记定位

李根桥,房体麟**,张宏涛,解超杰,杨作民,孙其信,刘志勇*   

  1. 中国农业大学植物遗传育种系/农业生物技术国家重点实验室/农业部作物基因组学与遗传改良重点开放实验室/教育部作物杂种优势研究与利用重点实验室,北京100193
  • 收稿日期:2008-12-04 修回日期:2009-02-17 出版日期:2009-05-12 网络出版日期:2009-03-20
  • 通讯作者: 刘志勇
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2006AA100102,2006AA10Z1E9,2006AA10Z1C4,2006BAD01A02),国家杰出青年科学基金项目(30425039),北京市自然科学基金(6061003),教育部长江学者和创新团队发展计划项目,高等到学校学科创新引智计划项目(111-2-03)资助。

Identification and SSR Mapping of Two Powdery Mildew Resistance Genes in Wild Emmer (Triticum dicoccoides) Accessions IW3 and IW10

LI Gen-Qiao,FANG Ti-Lin**,ZHANG Hong-Tao,XIE Chao-Jie,YANG Zuo-Min,SUN Qi-Xin,LIu Zhi-Yong   

  1. Department of Plant Genetics & Breeding,China Agricultural University/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, Beijing 100193,China
  • Received:2008-12-04 Revised:2009-02-17 Published:2009-05-12 Published online:2009-03-20
  • Contact: LIU Zhi-Yong

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被引次数

13

摘要：

野生二粒小麦(Triticum dicoccoides)是小麦抗病育种的重要资源库之一。来自以色列Mount Hermon的野生二粒小麦材料IW3 和IW10对我国小麦白粉病菌生理小种E09表现高抗。对硬粒小麦Langdon与IW3和IW10两个杂交组合F2分离群体和F3家系的遗传分析表明，IW3和IW10对小麦白粉菌E09的抗性均受显性单基因控制，暂被命名为MlIW3和MlIW10。采用BSA法和SSR标记分析，筛选到与抗白粉病基因MlIW3和MlIW10连锁的5个SSR标记，这两个基因均位于Xbarc84和Xwmc326之间，顺序为Xbarc84–4.6 cM–MlIW3–1.6 cM–Xwmc326和Xbarc84–6.6 cM–MlIW10–0.6 cM–Xwmc326。根据SSR分子标记的遗传图谱和在中国春的缺体—四体、双端体和缺失系的定位结果，这两个抗白粉病基因被定位在3BL染色体的末端。根据MlIW3和MlIW10的来源和分子标记定位结果，推断这两个基因可能是小麦抗白粉病基因Pm41或其等位基因或位于同一个基因簇中。

关键词: 野生二粒小麦, 抗白粉病基因, SSR标记

Abstract:

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a major wheat disease in the world. Deployment of resistant varieties is considered the most economical and effective way for controlling the disease. Wild emmer is one of the important genetic resources for wheat disease resistance genes. Two wild emmer accessions, IW3 and IW10, collected from Mount Hermon, Israel, are highly resistant to prevailing Bgt isolate E09. Genetic analyses of the F2 populations and F3 progenies derived from the crosses between Triticum durum cultivar Langdon and IW3 or IW10 indicated that each accession possessed a single dominant gene, temporarily designated MlIW3 and MlIW10, respectively, conferred resistance to Bgt isolate E09. Bulked segregant analysis (BSA) and SSR mapping revealed that both MlIW3 and MlIW10 were flanked by SSR markers Xbarc84 and Xwmc326 with genetic distances of Xbarc84–4.6 cM–MlIW3–1.6 cM–Xwmc326 and Xbarc84–6.6 cM–MlIW10–0.6 cM–Xwmc326. Both MlIW3 and MlIW10 were physically mapped on the distal bin of chromosome 3BL using Chinese Spring nullisomic–tetrasomic, ditelisomic, and deletion lines. According to the collecting geographic sites of IW3 and IW10 in Israel and the SSR mapping data, MlIW3 and MlIW10 appear to be the same or allelic to wild emmer derived powdery mildew resistance gene Pm41 or in the same cluster with it.

Key words: Wild emmer, Powdery mildew resistance genes, SSR marker

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