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作物学报 ›› 2009, Vol. 35 ›› Issue (5): 768-777.doi: 10.3724/SP.J.1006.2009.00768

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

强优势玉米杂交种雌穗发育期间的基因表达谱动态及重要功能基因

李波,张登峰,贾冠清,张体付,戴景瑞,王守才*   

  1. 中国农业大学国家玉米改良中心/农业部基因组学与遗传改良重点实验室/北京作物遗传改良重点实验室，北京100193
  • 收稿日期:2008-09-16 修回日期:2009-02-15 出版日期:2009-05-12 网络出版日期:2009-03-20
  • 通讯作者: 王守才,010-62732406
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2004AA211120和2007CB109003），国家高技术研究发展计划（863计划）项目（2004AA211120和2007AA10Z172）资助。

Gene Expression Profile and Main Function Genes during Ear Development in a Highly Heterotic Hybrid of Maize

LI Bo,ZHANG Deng-Feng,JIA Guan-Qing,ZHANG Ti-Fu,DAI Jing-Rri,WANG Shou-Cai*   

  1. National Maize Improvement Center of China/Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry/Key Laboratory of Genetic Improvement of Beijing City;China Agricultural University,Beijing 100193,China
  • Received:2008-09-16 Revised:2009-02-15 Published:2009-05-12 Published online:2009-03-20
  • Contact: WANG Shuo-Cai,010-62732406

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1907

被引次数

4

摘要：

利用基因芯片杂交方法，检测强优势玉米杂交种(C8605-2×W1445)雌穗发育过程中的基因表达动态，以期为进一步研究雌穗发育的分子机制提供证据。在雌穗到达小穗分化期之后8 d内，有671个基因的表达水平发生显著变化，主要表现出4种不同的表达模式。这些差异表达的基因涉及代谢、发育、刺激应答、细胞信号转导、物质运输等多个方面。细胞分裂基因、细胞壁结构和修饰蛋白基因在雌穗发育过程中大多表现为上调表达，可能对雌穗细胞的分化和果穗性状的形成产生重要影响。蛋白激酶、细胞信号转导以及转录因子基因等上游的信号传输和调控基因在雌穗的发育中也可能具有重要作用。

关键词: 芯片, 基因表达, 雌穗, 发育, 玉米

Abstract:

The development of ears is mostly responsible for the yield of maize (Zea mays L.), however, the molecular basis is unclear. To disclose the differential expression genes involved in the development of maize ear and their expression patterns, the gene expression at genome level of a highly heterotic hybrid (C8605-2×W1445) was detected from ear spikelet differentiation initiation to the later 8 d using microarrays with approximately 58 000 probes. The result of microarray analysis was verified using quantitative real-time PCR. A total of 671 genes expressed differentially which consisted of four expression patterns. These genes were involved in several biological processes, such as metabolism, development, responses to stimulus, cell signal transduction, and transport. Most genes for cell division, cell wall structure, and the modified protein of cell wall structure were identified to be upregulated during the ear development. Thus, these genes may play important roles in cell differentiation and formation of agronomic traits in maize ears. Moreover, genes for protein kinase, signal transduction, and transcription factors, which are involved in signal transduction and regulatory processes, may also take great functions in the development of maize ears.

Key words: Microarray, Gene expression, Ear, Development, Maize


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