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作物学报 ›› 2010, Vol. 36 ›› Issue (12): 2154-2161.doi: 10.3724/SP.J.1006.2010.02154

• 耕作栽培·生理生化 • 上一篇    下一篇

Cd2+对番茄幼苗生长和蛋白质组的影响

陈丽,王炼,王振英*,彭永康*   

  1. 天津师范大学生命科学学院 / 细胞遗传与分子调控天津市重点实验室，天津 300387
  • 收稿日期:2010-03-23 修回日期:2010-07-04 出版日期:2010-12-12 网络出版日期:2010-08-31
  • 通讯作者: 王振英,E-mail:wzycell@yahoo.com.cn; 彭永康,E-mail:pykcell@yahoo.com.cn
  • 基金资助:

    本研究由天津市科委重点项目(08JCZDJC16500)资助。

Effect of Cd2+ on Seedling Growth and Proteome in Tomato

CHEN Li,WANG Lian,WANG Zhen-Ying*,PENG Yong-Kang*   

  1. College of Life Sciences / Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin 300387, China
  • Received:2010-03-23 Revised:2010-07-04 Published:2010-12-12 Published online:2010-08-31
  • Contact: WANG Zhen-Ying,E-mail:wzycell@yahoo.com.cn;PENG Yong-Kang,E-mail:pykcell@yahoo.com.cn

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摘要： 以3 d龄番茄幼苗为试验材料, 从生理、生化和蛋白质组角度, 分析0.01~1.00 mmol L–1 Cd2+处理72 h后对幼苗的影响。结果表明, Cd2+处理导致幼苗生长严重受抑, 幼苗高度从对照组的4.76±0.50 cm分别降至3.79±0.05 cm (0.01 mmol L–1 Cd2+处理, P<0.01)和1.77±0.15 cm (0.03 mmol L–1 Cd2+处理, P<0.01)。根长度从对照组的6.07±0.04 cm降至4.77±0.58 cm (0.01 mmol L–1 Cd2+处理, P< 0.01)和3.65±0.66 cm (0.03 mmol L–1 Cd2+处理, P<0.01)。叶绿素含量在0.1 mmol L–1 Cd2+处理后开始下降。当幼苗用0.05 mmol L–1 Cd2+处理时, 根系中有10个蛋白质斑点, 叶片中有21个蛋白质斑点产生变化。利用MS/MS技术, 根系中有4个蛋白质斑点得以鉴别, 它们是ribosomal protein L 20 (斑点1)、F-box /LRR repeat protein (斑点2)、ribosomal protein small submit 4 (斑点4)和CBL-interacting protein kinase (斑点5)。在叶片中, 有2个蛋白质斑点消失, 4个蛋白质斑点合成, 它们是ABC transporter (斑点16)、maturase-like protein (斑点17)、chalcone synthase (斑点1)、a hypothetical protein (斑点3)、an unknown protein (斑点4)和a predicated protein (斑点6)。这些被鉴别的Cd2+反应蛋白参与生物合成、mRNA转录调控和蛋白质转运。

关键词: Cd2+胁迫, 蛋白质组, 部分氨基酸序列, MALDI-TOF-MS, 番茄

Abstract: Cadmium is one of the most serious heavy metal pollutions in agricultural soils in China. Three-day-old tomato seedlings were treated with 0.01–1.00 mmol L–1 Cd2+ for 72 h. The results showed that seedling growth was obviously inhibited and seedling height was decreased from 4.76±0.5 cm (in control) to 3.79±0.05 cm (in 0.01 mmol L–1 Cd2+ treatment, P<0.01) and 1.77±0.15 cm (in 0.03 mmol L–1 Cd2+ treatment, P<0.01). In addition, root length was also decreased from 6.07±0.04 cm (in control) to 4.77±0.58 cm (in 0.01 mmol L–1 Cd2+ treatment, P<0.01) and 3.65±0.66 cm (in 0.03 mmol L–1Cd2+ treatment, P<0.01). The chlorophyll contents decreased in the treatment with 0.1 mmol L–1 Cd2+. Ten protein spots in roots and twenty one protein spots in leaves were altered when the seedlings were treated with 0.05 mmol L–1 Cd2+. Total ten protein spots in roots were identified by MS/MS. Four new proteins were induced in roots, including spot 1: ribosomal protein L 20, spot 2: F-box /LRR repeat protein, spot 4: ribosomal protein small submit 4 and spot 5: CBL-interacting protein kinase. In the leaves, two protein spots disappeared and four new protein spots were induced, including spot 16, ABC transporter; spot 17, maturase-like protein; spot 1, chalcone synthase; spot 3, a hypothetical protein; spot 4, an unknown protein and spot 6, a predicated protein. These Cd2+ responsive proteins identified could be involved in protein biosynthesis, mRNA transcription regulation and protein transport. The above results showed that tomato is one of the highly sensitive crops to Cd2+ and could be used as a model to study the adaptation and tolerance mechanisms to heavy metals at physiological and biochemical levels.

Key words: Cd2+-stress, Proteome, Partial amino acid sequence, MALDI-TOF-MS, Tomato (Lycopersicon esculentum L.)

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