小麦茎秆木质素代谢及其与抗倒性的关系

doi:10.3724/SP.J.1006.2011.01616

摘要/Abstract

摘要： 以抗倒性不同的6个小麦品种为材料，观测了茎秆基部第二节间的木质素含量及苯丙氨酸转氨酶(PAL)、酪氨酸解氨酶(TAL)、肉桂醇脱氢酶(CAD)和4-香豆酸: CoA连接酶(4CL)活性以及不同时期茎秆抗折力和倒伏指数的变化，探讨木质素含量与抗倒伏能力的关系。不同小麦品种的茎秆木质素含量存在明显差异。茎秆抗折力大、倒伏指数小的品种(济麦20和济麦22)，其茎秆木质素含量高，PAL、TAL、CAD和4CL活性强。相关分析表明，茎秆木质素含量与实际倒伏率呈显著负相关(r = -0.83, P<0.05)；与抗折力呈显著正相关(r = 0.86, P<0.05)；PAL、TAL和CAD活性与木质素含量呈显著正相关(r =0.78, 0.77, 0.85, P<0.05)，4CL活性与木质素含量呈不显著正相关(r =0.39, P>0.05)。研究表明，较高的PAL和CAD活性是木质素含量高的酶学基础，茎秆木质素含量可作为小麦品种抗倒伏性评价的一个重要指标。

关键词: 冬小麦, 木质素代谢, 抗倒性, 倒伏指数, 酶活性

Abstract: As a component of cell wall, lignin is believed to be relevant to mechanical intensity of culm. In this study, we investigated the dynamic changes of lignin content and activities of four enzymes involved in lignin metabolism in culm as well as the snapping resistance and lodging index of wheat (Tritcum aestivum L.) to disclose the relationship between lignin content and lodging resistance. The lignin content and activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), cinnamyl alcohol dehydrogenase (CAD), and 4-coumarate: CoA ligase (4CL) were tested with the second internode of six cultivars with different lodging resistance. The snapping resistance and culm lodging index were determined at anthesis, milking, and maturity stages. The lignin content varied significantly among cultivars. Jimai 22 and Jimai 20, which showed high resistance to culm snapping and loging, had higher lignin content and the activities of PAL, TAL, CAD, and 4CL than other cultivars. The lignin content was negatively correlated with the actual lodging ratio (r = -0.83, P < 0.05), and positively correlated with the snapping resistance of culm (r = 0.86, P < 0.05). The activities of PAL, TAL, and CAD were positively correlated with lignin content with correlation coefficients of 0.78 (P < 0.05), 0.77 (P < 0.05) and 0.85 (P < 0.05), respectively. Based on these results, we conclude that high activities of PAL and CAD are the enzymatic basis of increased lignin content, and the lignin content in culm can be used as an important indicator to evaluate lodging resistance of wheat.

Key words: Winter wheat, Lignin metabolism, Lodging resistance, Lodging index, Enzyme activity

陈晓光, 史春余, 尹燕枰, 王振林, 石玉华, 彭佃亮, 倪英丽, 蔡铁. 小麦茎秆木质素代谢及其与抗倒性的关系[J]. 作物学报, 2011, 37(09): 1616-1622.

CHEN Xiao-Guang, SHI Chun-Tu, YIN Yan-Ping, WANG Zhen-Lin, SHI Yu-Hua, PENG Dian-Liang, NI Yang-Li, CAI Tie. Relationship between Lignin Metabolism and Lodging Resistance in Wheat[J]. Acta Agron Sin, 2011, 37(09): 1616-1622.

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