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作物学报 ›› 2012, Vol. 38 ›› Issue (05): 808-813.doi: 10.3724/SP.J.1006.2012.00808

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

疣粒野生稻泛素结合酶基因的全长cDNA序列克隆与分析

蒋春苗1,2，黄兴奇1，付坚1，余腾琼1，钟巧芳1，李定琴1，殷富有1，张敦宇1，王玲仙1，程在全1,*   

  1. 1 云南省农业科学院生物技术与种质资源研究所, 云南昆明650223；2 云南大学生命科学学院, 云南昆明650091
  • 收稿日期:2011-10-19 修回日期:2012-01-19 出版日期:2012-05-12 网络出版日期:2012-03-05
  • 通讯作者: 程在全, E-mail: czquan-99@163.com, Tel: 13888556318, 0871-5140200
  • 基金资助:

    本研究由国家自然科学基金项目(31160067, 31000746)，国家公益性行业科研专项(201003021)，云南省重点应用基础研究项目(2008C004)和云南省自然科学基金项目(2010CD098)资助

Cloning and Analysis on Full-length cDNA Sequence of Ubiquitin-conjugating Enzyme Gene from Oryza meyeriana Baill.

JIANG Chun-Miao1,2,HUANG Xing-Qi1,FU Jian1,YU Teng-Qiong1,ZHONG Qiao-Fang1,LI Ding-Qin1,YIN Fu-You1,ZHANG Dun-Yu1,WANG Ling-Xian1,CHENG Zai-Quan1,*   

  1. 1 Biotechnology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2 College of Life Science, Yunnan University, Kunming 650091, China
  • Received:2011-10-19 Revised:2012-01-19 Published:2012-05-12 Published online:2012-03-05
  • Contact: 程在全, E-mail: czquan-99@163.com, Tel: 13888556318, 0871-5140200

PDF

1265

被引次数

10

摘要： 从已构建疣粒野生稻消减cDNA文库中随机挑取阳性单克隆经测序得到ESTs, 通过生物信息学分析, 选取与泛素结合酶具有同源功能的EST以RACE技术分离克隆到1个疣粒野生稻泛素结合酶基因的全长cDNA序列, 命名为OmE2 (Oryza meyerianaBaill. ubiquitin-conjugating enzyme, OmE2), 该序列长917 bp, 最大开放阅读框为528 bp, 编码的蛋白具有175个氨基酸, 该蛋白的分子量为19.31 kD, 理论等电点为9.32。OmE2蛋白与其他物种的泛素结合酶具有高度的一致性和相似性, 含有泛素结合酶活性位点的保守序列及半胱氨酸残基, 且具有3个跨膜结构域, 推测OmE2是一类泛素结合蛋白兼跨膜蛋白。经RT-PCR分析, OmE2基因是受白叶枯病病原菌胁迫诱导表达的, 是首次从野生稻中发现的可能参与疣粒野生稻胁迫信号传导和抗病应答反应的基因。

关键词: 疣粒野生稻, 泛素结合酶, 序列分析

Abstract: Based on the bioinformatic analysis of the ESTs from the subtraction cDNA library of Oryza meyeriana, we cloned one of the ESTs which has the homolog function of ubiquitin-conjugating enzyme and got a full-length cDNA sequence of ubiquitin-conjugating enzyme gene by RACE, and named it OmE2 (Oryza meyerianaBaill ubiquitin-conjugating enzyme, OmE2). This full-length cDNA sequence has 917 bp including 528 bp open reading frame encoding a protein of 175 amino acids, with a predicted molecular mass of 19.3 kD and pI of 9.32. OmE2 protein which had significant amino sequence identity and similarity with ubiquitin-conjugating enzyme from other species contained the conserved sequence and cysteine residue in active sites of ubiquitin-conjugating family. OmE2 had three trans-membrane domains. It is predicted that OmE2 protein is a kind of ubiquitin-conjugating protein and transmembrane protein. The expression of OmE2 inOryza meyeriana was induced by Xanthomonas campestris pv. oryzae. Therefore OmE2 gene which was first found in wild rice may participate in the signal transduction and the resistance response in Oryza meyeriana.

Key words: Oryza meyeriana Baill., Ubiquitin-conjugating enzyme, Sequence analysis

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