欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (05): 794-800.doi: 10.3724/SP.J.1006.2010.00794

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

芥菜型油菜FAE1基因序列特征及其与芥酸含量关系的初步分析

徐爱遐1,2,黄镇2,马朝芝1,肖恩时2,张修森2,涂金星1,*,傅廷栋1   

  1. 1华中农业大学作物遗传改良国家重点实验室,湖北武汉430070;2西北农林科技大学农学院,陕西杨凌712100
  • 收稿日期:2009-10-13 修回日期:2010-02-07 出版日期:2010-05-12 网络出版日期:2010-03-15
  • 通讯作者: 涂金星, E-mail: tujx@mail.hzau.edu.cn; Tel: 027-87281819
  • 基金资助:

    本研究由陕西省自然科学基金项目(2005C108)，国家技术研究发展计划(863计划)项目(2009AA101105)和西北农林科技大学唐仲英育种基金(A212020523)资助。

FAE1 Sequence Characteristics and Its Relationship with Erucic Acid Content in Brassica juncea

 XU Ai-Xia1,2,HUANG Zhen2, MA Chao-Zhi1, XIAO En-Shi2, ZHANG Xiu-Sen2, TU Jin-Xing1*, FU Ting-Dong1   

  1. 1National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;2College of Agriculture,Northwest A&F University,Yangling 712100,china
  • Received:2009-10-13 Revised:2010-02-07 Published:2010-05-12 Published online:2010-03-15
  • Contact: TU Jin-Xing,E-mail:tujx@mail.hzau.edu.cn;Tel:027-87281819

PDF

1822

被引次数

10

摘要：

采用同源序列法对6个芥菜型油菜(Brassica juncea)品种(高芥酸、中芥酸、低芥酸)、2份白菜型油菜品种(高芥酸和低芥酸)和1份黑芥品种的FAE1基因进行克隆和测序表明，9个品种的FAE1基因编码区全长均为1 522 bp，不含内含子，均编码507个氨基酸残基。序列比较表明，芥菜型油菜中有两种FAE1基因序列(BjFAE1.1和BjFAE1.2)，其亲缘种白菜型油菜和黑芥中各有一种FAE1基因序列(BrFAE1和BnFAE1), BjFAE1.1对应于白菜型油菜的BrFAE1, BjFAE1.2对应于黑芥BnFAE1；BjFAE1.1和BjFAE1.2之间存在71处核苷酸变异和Hind III不同的酶切位点(第1415位和第1144位), 蛋白质水平上存在15处氨基酸变异。比较不同芥酸含量品种的FAE1基因序列表明，BjFAE1.1基因存在2个SNP位点(第968位和第1265位), BjFAE1.2基因也有2个SNP位点(第49位和第237位)，这4个SNP位点中有3个位点(第49位、第968位和第1 265位)导致蛋白质水平上氨基酸的差异。其中BjFAE1.1基因第968位的碱基变化(C→T)引起的第323位氨基酸变化(Thr→Ile)，能够解释芥菜型油菜和白菜型油菜高芥酸到低芥酸(中芥酸)的转变；第1265位的碱基变化(T→C)引起的第422位的氨基酸变化(Phe→Ser)，能够部分解释芥菜型油菜的高芥酸到低芥酸(中芥酸)的转变，白菜型油菜的高芥酸和低芥酸品种在该位点的碱基没有变化。BjFAE1.2基因第49位的碱基变化(T→C)引起的第17位氨基酸的变化(Phe→Leu)，可以解释芥菜型油菜的中芥酸变成高芥酸(低芥酸)。陕西黄芥低芥酸突变株1278-3的FAE1基因序列和国外低芥酸品种比较，只在第1 265位出现变异。

关键词: 芥菜型油菜, FAE1, 克隆, 序列分析

Abstract:

Low erucic acid content, which may result from the differential mutation of the fatty acid elongation 1 (FAE1) gene, is a major breeding target in canola quality improvement. We cloned the FAE1 genes in six Brassica juncea varieties (with high, intermediate and low contents of erucic acid), and their sibling species: two B. rapa (with high erucic acid and low contents of erucic acid) and one B. nigra varieties by using the method of homologous sequencing. The results showed that the sequences of FAE1 genes in nine varieties were 1 522 bp without introns, and all encoded a protein of 507 amino acids. There were two FAE1 gene sequences (BjFAEBjFAE1.1 and BjFAE1.2) in B. juncea, and only one FAE1 gene sequence was in B. rapa (BrFAE1) and B. nigra (BnFAE1), respectively. BjFAE1.1 was corresponding to the BrFAE1 of B. rapa; and BjFAE1.2 was corresponding to BnFAE1 of B. nigra. There were 71 nucleotide variations and the different HindIII restriction sites (No. 1415 and No. 1144) and 15 amino acid variations in protein construction between BjFAE1.1 and BjFAE1.2. The FAE1 gene sequences comparison analysis showed that there were two SNPs (No. 968 and No. 1265) in BjFAE1.1, two SNPs (No. 49 and No. 237) in BjFAE1.2, three of the four SNPs (No. 49, No. 968, and No. 1265) resulted in differences in the amino acid level. The No. 323 amino acid in BjFAE1.1 gene was changed (Thr → Ile) duo to the change of the No. 968 base (C→T)), which could explain the decrease of erucic acid content in B. juncea and B. rapa. The point mutation at the No. 1265 base (T→C) resulted in the change at No. 422 amino acid (Phe→Ser), which could partially explained partly explained the variations from high erucic acid to low erucic acid in Brassica juncea, but no difference was found in No. 1265 in B. rapa varieties with high and low erucic acid content. The mutation at No. 49 base (T→C) resulted in No.17 amino acid change (Phe→Leu), which could explained the variations from intermediate erucic acid to high erucic acid (erucic acid) in B. juncea. We compared the FAE1 gene sequences of the low erucic acid yellow mustard mutant called 1278-3 from northern Shaanxi and the varieties with low erucic acid from foreign countries, the results showed that their difference was only in No.1265 base.

Key words: Brassica juncea, FAEl, Cloning, Sequence analysis


[1]         Xu A-X(徐爱遐), Huang J-Y(黄继英). Analysis and evaluation of rapeseed germplasm resources in Shaanxi Province. J Northwest Agric (西北农业大学学报), 1999, 8(3): 89–92 (in Chinese with English abstract)


[2]         Lemieux B, Miquel M, Somerville C, Browse J. Mutants of Arabidopsis with alterations in seed lipid fatty acid composition. Theor Appl Genet, 1990, 80: 234–240


[3]         Kunst L, Taylor D C, Underhill E W. Fatty acid elongation in developing seeds of Arabidopsis thaliana. Plant Physiol Biochem, 1992, 30: 425–434


[4]         James D W, Lim E, Keller J, Plooy I, Ralston E, Dooner H K. Directed tagging of the Arabidopsis fatty acid elongation 1 (FAE1) gene with the maize transposon activator. Plant Cell, 1995, 7: 309–319


[5]         Clemens S, Kunst L. Isolation of Brassica napus cDNA encoding β-ketoacyl-CoA synthase, a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds. Plant Physiol, 1997, 115: 313–314


[6]         Barret P, Delourme R, Renard M, Domergue F, Lessire R, Delseny M, Roscoe T J. A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid. Theor Appl Genet, 1998, 96: 177–186


[8]         Han J, Lühs W, Sonntag K, Zähringer U, Borchardt D S, Wolter F P, Heinz E, Frentzen M. Functional characterization of β-ketoacyl-CoA synthase genes from Brassica napus L. Plant Mol Biol, 2001, 46: 229–239


[9]         Xiao L(肖玲), Lu C-M(卢长明). Cloning of fae1 gene partial sequence and SNP analysis in Brassica species. Sci Agric Sin (中国农业科学), 2005, 38(5): 891–896 (in Chinese with English abstract)


[10]      Wu Y-H(武玉花), Xiao L(肖玲), Wu G(吴刚), Lu C-M(卢长明). Cloning of FAE1 gene partial sequence and molecular analysis of A/C genomes in Brassica. Sci China (中国科学), 2007, 37(1): 35–41 (in Chinese)


[11]      Gupta V, Mukhopadhyay A, Arumugam N, Sodhi Y S, Pental D, Pradhan A K. Molecular tagging of erucic acid trait in oilseed mustard (Brassica juncea) by QTL mapping and single nucleotide polymorphisms in FAE1 gene. Theor Appl Genet, 2004, 108: 743–749
Doyle J J, Doyle J L. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 13–15
[1] 左同鸿, 张贺翠, 曾静, 朱利泉. 甘蓝自交不亲和相关基因BoPUB3L的克隆与表达分析[J]. 作物学报, 2026, 52(6): 1698-1710.
[2] 张曦, 王广恩, 李邵琦, 刘祎, 李俊兰, 钱玉源. 基于转录组测序解析陆海杂交姊妹系马克隆值差异的形成机制[J]. 作物学报, 2026, 52(5): 1442-1458.
[3] 余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432.
[4] 薛晓菲, 戴云静, 李熙林, 丁艳艳, 王翔, 雷长英, 韩焕勇, 贺道华. 陆地棉杜松烯合酶基因GhCDN10的特征及其在棉酚合成中功能分析[J]. 作物学报, 2025, 51(8): 2060-2076.
[5] 张金泽, 周庆国, 肖莉晶, 金海润, 欧阳青静, 龙旭, 晏中彬, 田恩堂. 芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J]. 作物学报, 2025, 51(5): 1166-1177.
[6] 王林, 陈晓雨, 张文梦龙, 汪思琦, 程冰云, 程靖秋, 潘锐, 张文英. 大麦HvMYB2分子特性及响应干旱胁迫的功能分析[J]. 作物学报, 2025, 51(4): 873-887.
[7] 张金泽, 周庆国, 杨旭, 王倩, 肖莉晶, 金海润, 欧阳青静, 余坤江, 田恩堂. 芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析[J]. 作物学报, 2025, 51(3): 621-631.
[8] 张正康, 苏延红, 阮孙美, 张敏, 张攀, 张慧, 曾千春, 罗琼. 疣粒野生稻中OgXa13的克隆和功能研究[J]. 作物学报, 2025, 51(2): 334-346.
[9] 李春梅, 陈洁, 郎兴宣, 庄海民, 朱靖, 杜梓君, 冯浩天, 金涵, 朱国林, 刘凯. 水稻矮化多分蘖基因DT1的图位克隆与功能分析[J]. 作物学报, 2025, 51(2): 347-357.
[10] 王玉娇, 王永乐, 添长久, 郁春旺, 吕佳斌, 朱加保. 薏苡VQ4基因的克隆及耐盐性初步分析[J]. 作物学报, 2025, 51(12): 3198-3210.
[11] 李世宽, 洪慧龙, 付佳祺, 谷勇哲, 孙如建, 邱丽娟. BSA-Seq结合RNA-Seq技术挖掘大豆叶片提前黄化衰老基因[J]. 作物学报, 2024, 50(2): 294-309.
[12] 胡瑶洁, 刘亚萍, 郑君妍, 韩婷, 马伯军, 叶亚峰, 刘斌美, 陈析丰. 水稻类病变早衰突变体lmes6的表型鉴定与基因定位[J]. 作物学报, 2024, 50(11): 2764-2774.
[13] 朱晓彤, 叶亚峰, 郭均瑶, 杨惠杰, 王紫瑶, 詹玥, 吴跃进, 陶亮之, 马伯军, 陈析丰, 刘斌美. 水稻早衰基因ESL8的遗传与定位[J]. 作物学报, 2023, 49(3): 662-671.
[14] 靖小菁, 杨新笋, 靳晓杰, 刘意, 雷剑, 王连军, 柴沙沙, 张文英, 焦春海. 甘薯蔓割病(Fusarium oxysporum f. sp. batatas)相关基因IbMAPKK9的克隆与特性分析[J]. 作物学报, 2023, 49(12): 3289-3301.
[15] 丁孟丽, 王茹茵, 施栋晟, 李莹博, 雷洁, 陈洪宇, 申清文, 王桂凤. 玉米小籽粒突变体mn-Mu的基因克隆与转录组分析[J]. 作物学报, 2023, 49(11): 3122-3130.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2