作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1119-1126.doi: 10.3724/SP.J.1006.2013.01119
陈明辉1,张保青1,宋修鹏1,陈虎3,杨丽涛1,2,*,李杨瑞1,2,*,陈保善1
CHEN Ming-Hui1,ZHANG Bao-Qing1,SONG Xiu-Peng1,CHEN Hu3,YANG Li-Tao1,2,*,LI Yang-Rui1,2*,CHEN Bao-Shan1
摘要:
宿根矮化病是危害严重的世界性甘蔗主要病害之一,为了探究甘蔗在该病胁迫下的抗病分子机制,本研究以甘蔗品种新台糖22健康植株为材料,利用含有宿根矮化病病原菌的蔗汁诱导其抗性,分别在接种后2、4、6、8和10 d取样,构建宿根矮化病侵染处理与对照的RNA混合池,利用cDNA-SCoT法进行差异显示研究。结果表明,80条SCoT引物扩增出500多条带,长度在100~1800 bp之间。从中筛选出30个差异明显的条带,测序后序列拼接,获得22条高质量的EST序列。生物信息学分析显示,对甘蔗宿根矮化病的差异基因主要涉及能量代谢、防卫反应、信号转导、蛋白质类代谢等方面。进一步基因功能分析显示,油菜素内酯合成蛋白、NBS-LRR类抗性蛋白、茉莉酸诱导蛋白、α-微管蛋白、ABA胁迫成熟蛋白、富含脯氨酸蛋白、翻译起始因子eif-2b α亚族等可能参与了甘蔗宿根矮化病病原菌互作的过程。
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