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作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1276-1283.doi: 10.3724/SP.J.1006.2013.01276

• 耕作栽培·生理生化 • 上一篇    下一篇

地黄栽培种与野生种内源激素含量的差异

李先恩1,2,孙鹏2,祁建军2,周丽莉2,王绍华1,*   

  1. 1 南京农业大学农学院，江苏南京 210095; 2 中国医学科学院北京协和医学院药用植物研究所, 北京 100094
  • 收稿日期:2012-06-25 修回日期:2013-03-11 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 王绍华, E-mail: wangsh@njau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(81274014)和国家转基因生物新品种培育重大专项(2012ZX09304006)资助。

Changes of Hormones in Cultivars and Wild-Type Varieties of Rehmannia glutinosa Libosch.

LI Xian-En1,2,SUN Peng2,QI Jian-Jun2,ZHOU Li-Li2,WANG Shao-Hua1,*   

  1. 1 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Medicinal Plant Development, China Academy of Medical Science, Peking Union Medical College, Beijing 100094, China
  • Received:2012-06-25 Revised:2013-03-11 Published:2013-07-12 Published online:2013-04-23
  • Contact: 王绍华, E-mail: wangsh@njau.edu.cn

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摘要：

块根的发育和形成与植物内源激素密切相关。本研究测定地黄2个栽培种和2个野生种在生长发育过程中内源激素含量表明，地黄叶片中IAA、ZR和ABA含量变化均呈单峰曲线，即在拉线期及快速增长期逐渐升高，之后逐渐降低，但各生育期栽培种叶片中IAA、ZR和ABA含量均高于野生种；栽培种与野生种块根中IAA和ZR含量变化规律明显不同，在拉线期与快速增长期，栽培种迅速上升，而后逐渐降低，而在整个生长期野生种变化不明显；整个生育期参试品种叶片中GA含量变化不大，栽培种在快速增长期根中GA、ABA的含量迅速上升，而野生种变化不明显。从上述变化规律来看，地黄块根的形成与发育是多种内源激素协同作用的结果。IAA和ZR是块根起始分化关键激素，决定了块根的形成，GA、ABA在地黄块根的发育与膨大中起了更重要的作用。

关键词: 地黄, 栽培种, 野生种, 植物激素

Abstract:

The formation and development of tuberous roots is related to plant endogenous hormones. We determined the contents of IAA, ZR, GA and ABA in leaves and roots of two cultivars and two wild-type varieties of Rehmannia glutinosa during the growth periods and compared their developmental changing trends between cultivars and wild-type varieties of Rehmannia glutinosa. The results showed that the contents of IAA, ZR, and ABA in leaves of cultivated Rehmannia glutinosa were higher than those of wild-type varieties and their changes in both cultivated and wild type of R. glutinosa displayed the same single-peak curves during the growth periods, they were all increased gradually at the elongation and rapid enlargement stages of roots and then decreased gradually. There was no significant developmental change in GA content in the leaves of both cultivated and wild-type varieties. Hormone contents in roots of cultivars had quite different developmental changes from those of wild-type varieties: The contents of IAA, ZR, and ABA in the cultivars presented the changing trend of single-peak curves during root growth periods while GA content increased rapidly in root enlargement stage. There was no much developmental change in IAA and GA contents in roots of wild-type varieties. ZR content decreased gradually with the root development in wild-type varieties. ABA content changed in roots of wild-type varieties displayed a single-peak curve which was much lower than that of the cultivars. This different developmental changes of hormone contents in rehmannia roots between cultivars and wild-type varieties indicated that the development of the rehmannia tuberous roots is affected by synergitic effects of several hormones and that IAA and ZR act on the initiation of tuberous roots, whereas GA and ABA play a more important role in root enlargement and thickening.

Key words: Rehmannia glutinosa Libosch, Cultivar, Wild-type varieties, Hormones

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