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作物学报 ›› 2016, Vol. 42 ›› Issue (04): 574-582.doi: 10.3724/SP.J.1006.2016.00574

• 耕作栽培·生理生化 • 上一篇    下一篇

水稻光氧化突变体812HS的光合和抗氧化特性

徐金刚1,吕川根2,*,刘莉1,吕春芳1,马静1,夏士健2,陈国祥1,高志萍1,*   

  1. 1 南京师范大学生命科学学院, 江苏南京210023；2 江苏省农业科学院粮食作物研究所, 江苏南京210014
  • 收稿日期:2015-08-21 修回日期:2016-01-11 出版日期:2016-04-12 网络出版日期:2016-01-27
  • 通讯作者: 吕川根, E-mail: lvchuangen@sina.com; 高志萍, E-mail: 08295@njnu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金(31271621), 江苏省普通高校自然科学研究计划(14KJB180011), 江苏高校优势学科建设工程(PAPD), 江苏省自然科学基金(BK20140916)和江苏省现代作物生产协同创新中心共同资助。

Characteristics of Photosynthesis and Antioxidation in Rice Photo-oxidation Mutant 812HS

XU Jin-Gang1,LÜ Chuan-Gen2,*,LIU Li1,LÜ Chun-Fang1,MA Jing1,XIA Shi-Jian2,CHEN Guo-Xiang1,GAO Zhi-Ping1,*   

  1. 1 College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; 2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2015-08-21 Revised:2016-01-11 Published:2016-04-12 Published online:2016-01-27
  • Contact: 吕川根, E-mail: lvchuangen@sina.com; 高志萍, E-mail: 08295@njnu.edu.cn
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31271621), Natural Science Research of Jiangsu Higher Education Institutions (14KJB180011), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Natural Science Foundation of Jiangsu Province (BK20140916), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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摘要：

本研究旨在通过梅雨前后自然光强明显变化的情况下对水稻叶片光氧化突变体812HS与野生型812S的PSII光化学活性、抗氧化酶活性等生理特性的比较研究，探讨812HS易受光氧化损伤现象的光合生理原因。结果表明，大田自然光照出现强光照前，812HS与812S的叶绿素含量、PSII活性、光合磷酸化活性、净光合速率、抗氧化酶活性和活性氧含量均无显著差异。自然强光照开始(梅雨结束)一段时间后，两种水稻的上述各项指标都发生了明显的变化。突变体812HS的叶绿素含量、PSII活性、非循环式光合磷酸化活性和净光合速率均较812S明显降低。高光强下812HS叶片中抗氧化酶CAT和POD活性的上升幅度则小于812S，从而使其体内富集了更多的O2?、H2O2和MDA。光氧化突变体812HS对高光照强度较敏感的PSII活性和较低的CAT、POD活性可能是其发生叶片光氧化现象的生理成因。

关键词: 光氧化, 光化学活性, 抗氧化酶活性

Abstract:

To reveal the physiological mechanism of leaf photo-oxidation in a rice mutant 812HS, we compared and analyzed differences of photochemical activity, antioxidative enzyme activities between 812HS and its wild type 812S grown in the field.  Results showed that chlorophyll content, PSII activity, photophosphorylation activity, net photosynthetic rate (Pn) and antioxidative enzymes activities as well as active oxygen contents in 812HS were similar to those in 812S before exposed to high intensity of sunlight. However, all above indexes were affected by a period of high intensity of sunlight. The decreased degree of chlorophyll content, PSII activity, non-cyclic photophosphorylation (NCPS) activity and Pn in 812HS were significantly higher than those in 812S. Besides, high intensity of sunlight led to a lower increase rate of POD and CAT activities in 812HS. Therefore, the contents of O2?, H2O2, and MDA in 812HS became higher than those in 812S. The result implied that the leaf photo-oxidation of mutant 812HS mainly results from higher sensitivity of PSII activities and lower CAT, POD activities under high intensity of sunlight.

Key words: Photo-oxidation, Photochemical activity, Antioxidative enzyme activity

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