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作物学报 ›› 2015, Vol. 41 ›› Issue (01): 136-144.doi: 10.3734/SP.J.1006.2015.00136

• 耕作栽培·生理生化 • 上一篇    下一篇

灌浆期高温对小麦旗叶与非叶器官光合和抗氧化酶活性的影响

张英华,杨佑明,曹莲,郝杨凡,黄菁,李金鹏,姚得秀,王志敏*   

  1. 中国农业大学农学与生物技术学院 / 农业部农作制重点开放实验室,北京 100193
  • 收稿日期:2014-03-11 修回日期:2014-09-30 出版日期:2015-01-12 网络出版日期:2014-11-11
  • 通讯作者: 王志敏, Email: zhimin206@263.net
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2012CB955904), 国家公益性行业(农业)科研专项(201303133), 国家自然科学基金项目(31401297), 国家科技支撑计划项目(2011BAD16B14), 国家现代农业产业技术体系建设专项(CARS-3)和北京市青年英才专项(31056101)资助。

Effect of High Temperature on Photosynthetic Capability and Antioxidant Enzyme Activity of Flag Leaf and Non-leaf Organs in Wheat

ZHANG Ying-Hua,YANG You-Ming,CAO Lian,HAO Yang-Fan,HUANG Jing,LI Jin-Peng,YAO De-Xiu,WANG Zhi-Min*   

  1. Key Laboratory of Farming System of Ministry of Agriculture / College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
  • Received:2014-03-11 Revised:2014-09-30 Published:2015-01-12 Published online:2014-11-11
  • Contact: 王志敏, Email: zhimin206@263.net

摘要:

为揭示小麦叶与非叶器官抗氧化系统对灌浆期高温胁迫的反应特征,探讨不同品种和不同器官耐热性差异机制,以小麦强耐热品种石家庄8号和弱耐热性品种河农341为材料,于灌浆期用塑料膜搭棚进行增温处理(花后第8天至第22),研究高温胁迫对旗叶光合速率(Pn)、叶绿素含量、旗叶和非叶器官中丙二醛(MDA)和脯氨酸(Pro)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响。高温处理下,两品种Pn比正常温度下(对照)18.7%~24.9%,叶绿素含量低5.7%~6.2%;旗叶、旗叶鞘、穗下节、颖片和籽粒的MDA含量和Pro含量均升高,其中MDA升高幅度为旗叶>非叶器官,Pro升高幅度为非叶器官>旗叶。旗叶、颖片、籽粒的SOD活性和旗叶、旗叶鞘、籽粒的CAT活性以及旗叶、旗叶鞘、颖片的POD活性在高温胁迫初期即诱导增强,而其他器官的抗氧化酶活性则在高温持续一段时间后诱导增强,之后随着高温的持续各器官抗氧化酶活性多表现为低于对照,高温解除后旗叶鞘、穗下节、颖片的SOD活性和旗叶、颖片、籽粒的POD活性有恢复迹象,高温对其他器官的SODPOD活性以及所有器官的CAT活性造成不可逆影响;总体来看,非叶器官持续抗氧化能力和耐热性强于叶片。石家庄8号叶与非叶器官细胞膜稳定性、抗氧化酶活性均高于河农341,显示其整株耐热性强于河农341,这是石家庄8号在高温胁迫下产量下降幅度低于河农341的重要生理基础。因此认为,非叶器官在小麦适应灌浆期高温逆境中发挥重要作用。

关键词: 小麦, 高温处理, 叶与非叶器官, 抗氧化酶活性, 耐热性

Abstract:

Winter wheat in North China has been subject to high temperature stress during grain filling. The purpose of this study was to determine the effect of high temperature on the photosynthetic capability of flag leaf and the antioxidant system of flag leaf and non-leaf organs in winter wheat cultivars Henong 341 (tolerant to high temperature) and Shijiazhuang 8 (sensitive to high temperature). High temperature (HT) stress was imposed with a plastic shed from the 8th to the 22nd day after anthesis, and normal temperature was used as the control. Under HT, the photosynthetic rate and chlorophyll content of flag leaf decreased by 18.7%–24.9% and 5.7%–6.2%, respectively; whereas the malodialdehyde (MDA) and proline (Pro) contents in flag leaf blade, sheath, peduncle, glume and grain increased in different levels. The increased percentage of MDA was higher in flag leaf than in non-leaf organs and that of Pro was higher in non-leaf organs than in flag leaf. Superoxide dismutase (SOD) in flag leaf, glume and grain, catalase (CAT) in flag leaf, sheath and grain, and peroxidases (POD) in flag leaf, sheath and glume were induced at early stage (4 d after treatment) by HT treatment, while the activities of antioxidant enzymes in other organs increased at later stage (7 d after treatment). Thereafter, the activities of antioxidant enzymes in various organs maintained lower levels compared with those of the control. At the 26th day after anthesis when HT stress was relieved for four days, the SOD activity in sheath, peduncle and glume and the POD activity in flag leaf, glume and grain began to increase, but the effects of HT on the SOD and POD activities in other organs and the CAT activity in all organs seemed irreversible. In general, the non-leaf organs exhibited higher antioxidant capability and heat tolerance than flag leaf. Compared with Henong 341, Shijiazhuang 8 exhibited higher cell membrane stability and antioxidant activity in leaf and non-leaf organs, leading to a tolerance to HT stress of the whole plant. This might be the physiological basis of smaller percentage of yield loss in Shijiazhuang 8 than in Henong 341 under HT stress. Our results indicate that non-leaf organs of wheat play an important role in adaptability to climate warming.

Key words: Wheat, High temperature, Leaf and non-leaf organs, Antioxidant enzyme activity, Heat tolerance

[1]北方十三省(市)小麦干热风科研协作组. 小麦干热风伤害机理的研究. 作物学报, 1984, 10: 105–112



The Cooperated Research Group on Dry-hot-wind Injury in Wheat in Thirteen Provinces and Municipalities in North China. Research in mechanism of wheat hot wind damage. Acta Agron Sin, 1984, 10: 105–112 (in Chinese with English abstract)



[2]Farooq M, Bramley H, Palta J A. Heat stress in wheat during reproductive and grain filling phases. Crit Rev Plant Sci, 2011, 30: 1–7



[3]邹琦. 小麦高温伤害与高温适应. 植物学报, 1988, 30: 388–395



Zou Q. Heat injury and heat acclimation in wheat plants. Acta Bot Sin, 1988, 30: 388–395 (in Chinese with English abstract)



[4]Shah N H, Paulsen G M. Interaction of drought and high temperature on photosynthesis and grain-filling of wheat. Plant Soil, 2003, 257: 219–226



[5]Plaut Z, Butow B J, Blumenthal C S, Wrigley C W. Transport of dry matter into developing wheat kernels and its contribution to grain yield under post-anthesis water deficit and elevated temperature. Field Crops Res, 2004, 86: 185–198



[6]郑飞, 臧秀旺, 黄保荣, 何钟佩. 灌浆期高温胁迫对冬小麦叶源、库器官生理活性的影响及调控. 华北农学报, 2001, 16 (2): 99–103



Zheng F, Zang X W, Huang B R, He Z P. Effects of high temperature stress on the source and sink organ of winter wheat during filling stage and its regulation. Acta Agric Boreali-Sin, 2001, 16(2): 99–103 (in Chinese with English abstract)



[7]郭天财, 王晨阳, 朱云集, 王化岑, 李九星, 周继泽. 后期高温对冬小麦根系及地上部衰老的影响. 作物学报, 1998, 24: 957–962



Guo T C, Wang C Y, Zhu Y J, Wang H C, Li J X, Zhou J Z. Effects of high temperature on the senescene of root and top-partial of wheat plant in the later stage. Acta Agron Sin, 1998, 24: 957–962 (in Chinese with English abstract)



[8]Almeselmani M, Deshmukh P S,Sairam R K. Protective role of antioxidant enzymes under high temperature stress. Plant Sci, 2006, 171: 382–388



[9]Sharkova V E, Bubolo L S. Effect of heat stress on the arrangement of thylakoid membranes in the chloroplasts of mature wheat leaves. Russ J Plant Physiol, 1996, 43: 358–365



[10]Dai H P, Zhang P P, Lu C, Jia G L, Song H, Ren X M, Chen J, Wei A Z, Feng B L, Zhang S Q. Leaf senescence and reactive oxygen species metabolism of broomcorn millet (Panicum miliaceum L.) under drought condition. Aust J Crop Sci, 2011, 5: 1655–1660



[11]刘萍, 郭文善, 浦汉春, 封超年, 朱新开, 彭永欣. 灌浆期高温对小麦剑叶抗氧化酶及膜脂过氧化的影响. 中国农业科学, 2005, 38: 2403–2407



Liu P, Guo W S, Pu H C, Feng C N, Zhu X K, Peng Y X. Effects of high temperature during grain filling period on antioxidant enzymes and lipid peroxidation in flag leaves of wheat. Sci Agric Sin, 2005, 38: 2403–2407 (in Chinese with English abstract)



[12]姜春明, 尹燕枰, 刘霞, 王振林. 不同耐热性小麦品种旗叶膜脂过氧化和保护酶活性对花后高温胁迫的响应. 作物学报, 2007, 33: 143–148



Jiang C M, Yin Y P, Liu X, Wang Z L. Response of flag leaf lipid peroxidation and protective enzyme activity of wheat cultivars with different heat tolerance to high temperature stress after anthesis. Acta Agron Sin, 2007, 33: 143–148 (in Chinese with English abstract)



[13]王志敏, 张英华, 张永平, 吴永成. 麦类作物穗器官的光合性能研究进展. 麦类作物学报, 2004, 24(4): 136–139



Wang Z M, Zhang Y H, Zhang Y P, Wu Y C. Review on photosynthetic performance of ear organs in Triticeae crops. J Triticeae Crops, 2004, 24(4): 136–139 (in Chinese with English abstract)



[14]徐晓玲, 王志敏, 张俊平. 灌浆期热胁迫对小麦不同绿色器官光合性能的得影响. 植物学报, 2001, 43: 571–577



Xu X L, Wang Z M, Zhang J P. Effect of heat stress on photosynthetic characteristics of different green organs of winter wheat during grain-filling Stage. Acta Bot Sin, 2001, 43: 571–577 (in Chinese with English abstract)



[15]Maydup M L, Antonietta M, Graciano C, Guiamet J J, Tambussi E A. The contribution of the awns of bread wheat (Triticum aestivum L.) to grain filling: Responses to water deficit and the effects of awns on ear temperature and hydraulic conductance. Field Crops Res, 2014, 167:102–111



[16]Xu X L, Zhang Y H, Wang Z M. Effect of heat stress during grain filling on phosphoenolpyruvate carboxylase.and ribulose-1,5-bisphosphate carboxylase/oxygenase activities of various green organs in winter wheat. Photosynthetica, 2003, 42: 317–320



[17]Tambussi E A, Bort J, Guiamet J J, Nogués S, Araus J L. The photosynthetic role of ears in C3 cereal: metabolism, water use efficiency and contribution to grain yield. Crit Rev Plant Sci, 2007, 26: 1-16



[18]中国科学院上海植物生理研究所、上海植物生理学会. 现代植物生理学实验指南. 北京: 科学出版社, 1999



Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai Association of Plant Physiology. A Laboratory Guide for Model Plant Physiology. Beijing: Science Press, 1999 (in Chinese)



[19]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2003. pp 195–197



Li H S. Experimental Theory and Technology in Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2003. pp 195–197 (in Chinese)



[20]陈金峰, 王宫南, 程素满. 过氧化氢酶在植物胁迫响应中的功能研究进展. 西北植物学报, 2008, 28: 188–193



Chen J F, Wang G N, Cheng S M. Progress about catalase function in plant stress reactions. Acta Bot Boreali-Occident Sin, 2008, 28: 188–193 (in Chinese with English abstract)



[21]田国忠, 李怀方, 裘维蕃. 植物过氧化物酶研究进展. 武汉植物学报研究, 2001, 19: 332–344



Tian G Z, Li H F, Qiu W F. Advances on Research of Plant Peroxidases. J Wuhan Bot Res, 2001, 19: 332–344 (in Chinese with English abstract)



[22]魏炜, 赵欣平, 吕辉, 刘克武, 喻东. 三种抗氧化酶在小麦抗干旱逆境中的作用初探. 四川大学学报(自然科学版), 2003, 40: 1172–1175



Wei W, Zhao X P, Lü H, Liu K W, Yu D. The Study of the function of three antioxidant enzymes in wheat leaf under drought stress. J Sichuan Univ (Nat Sci Edn), 2003, 40: 1172–1175 (in Chinese with English abstract)



[23]马旭俊, 朱大海. 植物超氧化物歧化酶(SOD)的研究进展. 遗传, 2003, 25: 225–231



Ma X J, Zhu D H. Functional roles of the plant superoxide dismutase. Hereditas (Beijing), 2003, 25: 225–231 (in Chinese with English abstract)

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