作物学报 ›› 2018, Vol. 44 ›› Issue (12): 1809-1817.doi: 10.3724/SP.J.1006.2018.01809
Jing DONG1,Xiao-Ping LU1,*(),Kun-Ming ZHANG1,Chun-Lei XUE1,Rui-Xia ZHANG2
摘要:
为探究高丹草杂种及其亲本间单核苷酸变异与其杂种优势形成的关系, 以高丹草杂种及其亲本的根、茎和叶组织为试验材料, 采用Illumina Hiseq 2000进行转录组测序。对平均长度达58 122 160 bp的各测序样品序列信息进行检测后, 均检测到不少于58 000个SNP位点, 位于基因内的SNP个数显著多于位于基因间的SNP个数, SNP发生频率为1/741 bp, 平均转换颠换比为1.00∶1.53。在所有变异类型中, C/T和G/A发生频率最高。经过筛选得到198 (21%)个极显著偏向性等位基因表达偏向性SNP, 其中65%偏向父本白壳苏丹草, 并且很多在白壳苏丹草中具有高水平基因表达的转录本, 在高丹草杂种中的等位基因表达也偏向白壳苏丹草。在3种组织中, 分别有79%、78%和82%转录本的2个亲本等位基因表现出相对平衡的表达水平, 说明与顺式作用相比, 反式作用可能更多地影响了等位基因的特异性表达。选择6个极显著偏向性等位基因表达偏向性SNP-unigene进行qRT-PCR验证, 这些基因的差异基因表达模式与RNA-Seq分析结果一致。本研究采用Illumina 测序技术研究等位基因表达, 为高丹草杂种优势分析提供了依据, 也为其他饲草作物的相关研究提供了理论参考。
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