作物学报 ›› 2020, Vol. 46 ›› Issue (3): 440-447.doi: 10.3724/SP.J.1006.2020.94080
刘震宇,王桂霞,李丽楠,蔡泽洲,梁潘潘,吴莘玲,张祥,陈德华()
Zhen-Yu LIU,Gui-Xia WANG,Li-Nan LI,Ze-Zhou CAI,Pan-Pan LIANG,Xin-Ling WU,Xiang ZHANG,De-Hua CHEN()
摘要:
以大面积生产应用的Bt棉常规种‘泗抗1号’和杂交种‘泗抗3号’为材料, 于扬州大学遗传生理重点实验室人工气候室, 以盆栽方式设计高温胁迫试验。2017年设蕾期38℃高温持续胁迫72 h终止、2018年设38℃高温持续胁迫96 h终止处理后不同时间(0、12、24、48、72和96 h)研究棉蕾Bt杀虫蛋白表达量变化及相关氮代谢生理。结果表明, 盛蕾期38℃持续胁迫72 h、96 h终止后棉蕾Bt杀虫蛋白表达量均能恢复到相应对照水平, 并且恢复所需时间随胁迫持续时间的延长而增加。胁迫72 h终止后, 常规种泗抗1号经72 h、杂交种泗抗3号经48 h蕾的Bt杀虫蛋白表达量与对照已无显著差异; 胁迫96 h终止后, 泗抗1号经96 h、泗抗3号经72 h, 棉蕾Bt杀虫蛋白表达量与对照已无显著差异。相关分析表明, 高温胁迫终止后, 蕾中可溶性蛋白表达量、谷氨酸丙酮酸转氨酶(GPT)活性、谷氨酸草酞乙酸转氨酶(GOT)活性与Bt杀虫蛋白表达量呈极显著正相关 (相关系数分别为0.964 **、0.981 **、0.971 **); 而游离氨基酸含量、蛋白酶和肽酶的活性与Bt杀虫蛋白表达量呈极显著负相关(相关系数分别为-0.894 **、-0.912 **、-0.834 **)。因此, 生产上可根据高温持续时间预测棉蕾Bt杀虫蛋白表达量的恢复程度, 合理防治棉铃虫等相关害虫。
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