欢迎访问作物学报,今天是

作物学报 ›› 2016, Vol. 42 ›› Issue (12): 1844-1852.doi: 10.3724/SP.J.1006.2016.01844

• 耕作栽培·生理生化 • 上一篇    下一篇

光、氮及其互作对玉米氮素吸收利用和物质生产的影响

宋航,周卫霞,袁刘正,靳英杰,李鸿萍,杨艳,尤东玲,李潮海*   

  1. 河南农业大学农学院 / 农业部玉米区域技术创新中心,河南郑州450002
  • 收稿日期:2016-02-04 修回日期:2016-06-20 出版日期:2016-12-12 网络出版日期:2016-07-28
  • 通讯作者: 李潮海, E-mail: lichaohai2005@163.com, Tel: 0371-63555629
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-02-19)和国家公益性行业(农业)科研专项(201203100)资助。

Effects of Light, Nitrogen and Their Interaction on Nitrogen Absorption, Utilization and Matter Production of Maize

SONG Hang,ZHOU Wei-Xia,JIN Ying-Jie,LI Hong-Ping,YANG Yan,YOU Dong-Ling,LI Chao-Hai*   

  1. Agronomy College, Henan Agricultural University, Zhengzhou 450002, China?
  • Received:2016-02-04 Revised:2016-06-20 Published:2016-12-12 Published online:2016-07-28
  • Contact: 李潮海, E-mail: lichaohai2005@163.com, Tel: 0371-63555629
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-02-19) and r the Special Fund for Agro-scientific Research in the Public Interest (201203100).

摘要:

以玉米单交种豫玉22为材料,设置2个光照处理和3个氮肥水平,研究光、氮及其互作下玉米酶活性、干物质生产和产量变化特征及其对玉米氮素吸收利用和物质生产的影响。结果表明,弱光胁迫下玉米叶片硝酸还原酶和谷氨酰胺合成酶活性降低,植株和籽粒氮积累量下降;干物质积累量显著降低;果穗穗长、行粒数和穗粒数减少,导致产量显著降低。但弱光胁迫下增施氮肥可以提高叶片硝酸还原酶和谷氨酰胺合成酶活性,增加干物质积累量,穗长、行粒数和穗粒数增加,产量显著提高,并且随施氮量的增多,产量增加效果也越显著。可见,光、氮及其互作对玉米氮素吸收利用及物质生产具有显著影响,弱光胁迫条件下增施氮肥可以部分缓解其致害效应,减少玉米产量损失。

关键词: 玉米, 氮肥水平, 弱光胁迫, 产量, 氮代谢, 物质生产

Abstract:

A field experiment was conducted using maize cultivar Yuyu 22 with three nitrogen levels (N1: 0 kg ha-1, N2: 120 kg ha-1, N3: 240 kg ha-1) and two light conditions (L1: no shading, L2: shading 14 days from the third day before tasseling) to study the effects of light, nitrogen and their interaction on nitrogen absorption and utilization, matter production of maize in 2012 and 2013. Compared with L1 treatment, L2 not only reduced the activities of nitrate reductase (NR) and glutamine synthetase (GS) in ear leaf and nitrogen accumulation in plant and grain, but also significantly reduced dry matter accumulation and declined the length of ear, kernels per row and kernels per ear, resulting in the final grain yield reduced significantly. However under L2, with increasing nitrogen application level, the activities of NR and GS in ear leaf improved and ear length, kernels per row and kernels per ear increased significantly, the grain yield eventually improved significantly. This indicates that there are significant effects of light, nitrogen and their interaction on matter production , nitrogen absorption and utilization of maize, and that nitrogen fertilizer could partially offset the impact of low-light stress on the matter production and yield of maize.

Key words: Maize, Nitrogen levels, Low-light stress, Yield, Nitrogen absorption and utilization, Matter production

[1] 李冬梅, 张春玲, 吴亚男, 赵奎华, 王延波, 齐华, 聂林雪, 陈涛, 马丽婷. 不同耐密性玉米品种干物质积累及产量对弱光响应的差异. 玉米科学, 2013, 21: 54–58
Li D M, Zhang C L, Wu Y N, Zhao K H, Wang Y B, Qi H, Nie L X, Chen T, Ma L T. Effect of low-light on the dry matter accumulation, distribution and yield of different maize varieties with density-tolerance. J Maize Sci, 2013, 21: 54–58 (in Chinese with English abstract)
[2] Tohru K, Makoto S, Sadanori T. Shading during the early grain filling period does not affect potential grain dry matter increase in rice. Agron J, 92: 411–417
[3] Reed A J, Singletary G W, Schussler J R, Williamson D R, Christy A L. Shading effects on dry matter and nitrogen partitioning, kernel number, and yield of maize. Crop Sci, 1987, 28: 819–825
[4] Reed A J, Singletary G W. Roles of carbohydrate supply and phytohormones in maize kernel abortion. Plant Physiol, 1989, 91: 986–992
[5] 张吉旺, 董树亭, 王空军, 胡昌浩, 刘鹏. 遮阴对夏玉米生长发育和产量的影响. 应用生态学报, 2006, 17: 657–662
Zhang J W, Dong S T, Wang K J, Hu C H, Liu P. Effects of shading on the growth, development and grain yield of summer maize. Chin J Appl Ecol, 2006, 17: 657–662 (in Chinese with English abstract)
[6] 赵洪利, 褚丽敏, 王焕军. 弱光胁迫对玉米产量相关性状的影响. 现代化农业, 2014, 423: 1–2
Zhao H L, Zhu L M, Wang H J. Effects of low-light stress on yield traits of maize. Modern Agric, 2014, 423: 1–2 (in Chinese)
[7] 付景, 李潮海, 赵久然, 刘天学. 弱光条件下不同玉米品种净光合速率及产量和品质的比较研究. 河南农业大学学报, 2009, 43: 130–134
Fu J, Li C H, Zhao J R, Liu T X. Comparison of photosynthetic rate, grain yield and quality of different maize hybrids under low-light conditions. J Henan Agric Univ, 2009, 43: 130–134 (in Chinese with English abstract)
[8] 贾士芳, 董树亭, 王空军, 张吉旺, 李从锋. 玉米花粒期不同阶段遮光对籽粒品质的影响. 作物学报, 2007, 33: 1960–1967
    Jia S F, Dong S T, Wang K J, Zhang J W, Li C F. Effect of shading on grain quality at different stages from flowering to maturity in maize. Acta Agron Sin, 2007, 33: 1960–1967 (in Chinese with English abstract)
[9] Kiniry J R, Ritchie J T. Shade-sensitive interval of kernel number of maize. Agron J, 1985, 77: 711–715
[10] 姜涛. 氮肥运筹对夏玉米产量、品质及植株养分含量的影响. 植物营养与肥料学报, 2013, 19: 559–565 
     Jiang T. Effects of nitrogen application regime on yield, quality and plant nutrient contents of summer maize. Plant Nutr Fert Sci, 2013, 19: 559–565 (in Chinese with English abstract)
[11] 宋尚有, 王勇, 樊廷录, 高育锋, 唐小明, 李尚中. 氮素营养对黄土高原旱地玉米产量、品质及水分利用效率的影响. 植物营养与肥料学报, 2007, 13: 387–392
Song S Y, Wang Y, Fan T L, Gao Y F, Tang X M, Li S Z. Effect of nitrogen fertilizer on grain yield, quality and water use efficiency of corn in dry land of Loess Plateau. Plant Nutr Fert Sci, 2007, 13: 387–392 (in Chinese with English abstract)
[12] 赵营, 同延安, 赵护兵. 不同供氮水平对夏玉米养分累积、转运及产量的影响. 植物营养与肥料学报, 2006, 12: 622–627
Zhao Y, Tong Y A, Zhao H B. Effects of different N rates on nutrients accumulation, transformation and yield of summer maize. Plant Nutr Fert Sci, 2006, 12: 622–627 (in Chinese with English abstract)
[13] 申丽霞, 王璞, 张软斌. 施氮对不同种植密度下夏玉米产量及子粒灌浆的影响. 植物营养与肥料学报, 2005, 11: 314–319
Shen L X, Wang P, Zhang R B. Effect of nitrogen supply on yield and grain filling in summer maize with different crop density. Plant Nutr Fert Sci, 2005, 11: 314–319 (in Chinese with English abstract)
[14] 申丽霞, 魏亚萍, 王璞, 易镇邪, 张红芳, 兰林旺. 施氮对夏玉米顶部籽粒早期发育及产量的影响. 作物学报, 2006, 32: 1746–1751
Shen L X, Wei Y P, Wang P, Yi Z X, Zhang H F, Lan L W. Effect of nitrogen supply on early kernel development and yield in summer maize (Zea mays L.). Acta Agron Sin, 2006, 32: 1746–1751 (in Chinese with English abstract)
[15] 王俊忠, 黄高宝, 张超男, 杨亚军, 赵会杰, 朱晓燕, 马培芳. 施氮量对不同肥力水平下夏玉米碳氮代谢及氮素利用率的影响. 生态学报, 2009, 29: 2045–2052
Wang J Z, Huang G B, Zhang C N, Yang Y J, Zhao H J, Zhu X Y, Ma P F. Influence of nitrogen fertilizer rate on carbon-nitrogen metabolism and nitrogen use efficiency of summer maize under high and medium yield levels. Acta Ecol Sin, 2009, 29: 2045–2052 (in Chinese with English abstract)
[16] 申丽霞, 王璞, 兰林旺, 孙西欢. 施氮对夏玉米碳氮代谢及穗粒形成的影响. 植物营养与肥料学报, 2007, 13: 1074–1079 
Shen L X, Wang P, Lan L W, Sun X H. Effect of nitrogen supply on carbon-nitrogen metabolism and kernel set in summer maize. Plant Nutr Fert Sci, 2007, 13: 1074–1079 (in Chinese with English abstract)
[17] 李从锋, 王空军, 刘鹏, 张吉旺, 董树亭. 供氮水平对雄性不育玉米物质生产和氮代谢的影响. 植物营养与肥料学报, 2009, 15: 99–104 
     Li C F, Wang K J, Liu P, Zhang J W, Dong S T. Effect of nitrogen rate on matter production and nitrogen metabolism traits of cytoplasmic male sterility maize (Zea mays L.). Plant Nut Fert Sci, 2009, 15: 99–104 (in Chinese with English abstract)
[18] 关义新, 林葆, 凌碧莹. 光、氮及其互作对玉米幼苗叶片光合和碳、氮代谢的影响. 作物学报, 2000, 26: 806–812
Guan Y X, Lin B, Ling B Y. The interactive effects of growth light condition and nitrogen supply on maize (Zea mays L.) seedling photosynthetic traits and metabolism of carbon and nitrogen. Acta Agron Sin, 2000, 26: 806–812 (in Chinese with English abstract)
[19] 徐虹, 张丽娟, 赵艳霞, 姜蓝齐. 黄淮海地区夏玉米花期阴雨灾害风险区划. 自然灾害学报, 2014, 23: 263–272
Xu H, Zhang L J, Zhao Y X, Jiang L Q. Risk zoning of continuous overcast rain disaster of summer maize during florescence in Huang-Huai-Hai region. J Nat Disasters, 2014, 23(5): 263–272 (in Chinese with English abstract)
[20] 付景, 李潮海, 赵久然, 马丽, 刘天学. 玉米品种耐阴性指标的筛选与评价. 应用生态学报, 2009, 20: 2705–2709
Fu J, Li C H, Zhao J R, Ma L, Liu T X. Shade-tolerance indices of maize: Selection and evaluation. Chin J Appl Ecol, 2009, 20: 2705–2709 (in Chinese with English abstract)
[21] 鲍士旦. 土壤农化分析(第3版). 北京: 中国农业出版社, 2000. pp 44–49
Bao S D. Analysis of Soil and Agricultural Chemistry, 3rd edn. Beijing: China Agriculture Press, 2000. pp 44–49 (in Chinese)
[22] 王学奎. 植物生理生化实验原理和技术(第2版). 北京: 高等教育出版社, 2006. pp 126–127
Wang X K. Principles and Techniques of Plant Physiological Biochemical Experiment, 2nd edn . Beijing: Higher Education Press, 2006. pp 126–127 (in Chinese)
[23] 邹琦. 植物生理学实验指导. 北京: 中国农业出版社, 2000. pp 184–192
Zou Q. Instruction of Plant Physiology Experiment. Beijing: China Agriculture Press, 2000. pp 184–192 (in Chinese)
[24] Esteban S, Rosa M R, Juan M R, Luis R. Changes in biomass, enzymatic activity and protein concentration in roots and leaves of green bean plants (Phaseolus vulgaris L. cv. Strike) under high NH4NO3 application rates. Sci Hortic, 2004, 99: 237–248
[25] Moro B G, Petite A M, Lacuesta M, Murua C G, Rueda A M. Glutamine synthetase from mesophyll and hundle sheath maize cells: isoenzyme complements and different sensitivities to phosphinothricin. Plant Cell Rep, 2000, 19: 1127–1134
[26] 全晓艳. 光氮互作对水稻干物质生产和碳、氮代谢的影响. 扬州大学硕士学位论文, 2013. pp 3–4
Quan X Y. Interactive Effects of Light Intensity and Nitrogen Supply on Dry Matter Production and Carbon-Nitrogen Metabolism of Rice. MS Thesis of Yangzhou University, 2013. pp 3–4 (in Chinese)
[27] 耿玉辉, 李刚, 曹秀艳, 李春艳, 曹国军. 氮、钾不同营养水平对春玉米氮代谢的影响. 玉米科学, 2009, 17: 101–104
Geng Y H, Li G, Cao X Y, Li C Y, Cao G J. Effects of different N and K levels on nitrogen metabolism of spring maize. J Maize Sci, 2009, 17: 101–104 (in Chinese with English abstract)
[28] 申丽霞, 王璞. 玉米穗位叶碳氮代谢的关键指标测定. 中国农学通报, 2009, 25: 155–157
Shen L X, Wang P. Determination of C-N metabolism indices in ear leaf of maize (Zea mays L.). Chin Agric Sci Bull, 2009, 25: 155–157 (in Chinese with English abstract)
[29] 周晓琳, 薛登峰, 刘树堂, 宋希云. 长期定位施肥对夏玉米氮代谢的影响. 中国农学通报, 2009, 25: 85–88
Zhou X L, Xue D F, Liu S T, Song X Y. Effect on nitrogen metabolism character of summer maize under long-term locating fertilization. Chin Agric Sci Bull, 2009, 25: 85–88 (in Chinese with English abstract)
[30] 李潮海, 栾丽敏, 尹飞, 王群, 赵亚丽. 弱光胁迫对不同基因型玉米生长发育和产量的影响. 生态学报, 2005, 25: 824–830
Li C H, Luan L M, Yin F, Wang Q, Zhao Y L. Effects of light stress at different stages on the growth and yield of different maize genotypes (Zea mays L.). Acta Ecol Sin, 2005, 25: 824–830 (in Chinese)
[31] 史建国, 崔海岩, 赵斌, 董树亭, 刘鹏, 张吉旺. 花粒期光照对夏玉米产量和籽粒灌浆特性的影响. 中国农业科学, 2013, 46: 4427–4434
Shi J G, Cui H Y, Zhao B, Dong S T, Liu P, Zhang J W. Effect of light on yield and characteristics of grain-filling of summer maize from flowering to maturity. Sci Agric Sin, 2013, 46: 4427–4434 (in Chinese with English abstract)
[32] 王楚楚, 高亚男, 张家玲, 曹庆军, 陈乾隆, 陈红强, 熊萍, 毛庆明, 崔金虎. 种植行距对春玉米干物质积累与分配的影响. 玉米科学, 2011, 19: 108–111
Wang C C, Gao Y N, Zhang J L, Cao Q J, Chen Q L, Chen H Q, Xiong P, Mao Q M, Cui J H. Effect of row spacing on the regularity of accumulation and distribution of dry matter in spacing maize. J Maize Sci, 2011, 19: 108–111 (in Chinese with English abstract)
[33] 徐祥玉, 张敏敏, 翟丙年, 李生秀. 施氮对不同基因型夏玉米干物质累积转移的影响. 植物营养与肥料学报, 2009, 15: 786–792
Xu X Y, Zhang M M, Zhai B N, Li S X. Effects of nitrogen application on dry matter accumulation and translocation of different genotypes of summer maize. Plant Nutr Fert Sci, 2009, 15: 786–792 (in Chinese with English abstract)

[1] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2] 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[3] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[4] 马胜乾, 王志平, 陈浩天, 窦淑贤, 张燕, 邓艾兴, 张卫建, 原向阳, 宋振伟. 秸秆还田下耕作方式与氮肥施用量对东北玉米产量及土壤团聚体的影响[J]. 作物学报, 2026, 52(6): 1802-1816.
[5] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[6] 张思思, 赵向辉, 周洋, 姚云凤, 朱荣昱, 董元杰, 胡国庆, 徐通, 刘兆新. 冬闲期翻耕和绿肥还田对连作花生田土壤理化性质和产量的影响[J]. 作物学报, 2026, 52(5): 1472-1486.
[7] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[8] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[9] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[10] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[11] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[12] 刘昕萌, 任昊, 张继波, 张吉旺, 赵斌, 任佰朝, 刘鹏, 王洪章. 茉莉酸甲酯(MeJA)缓解高温影响玉米雌穗分化的生理机制[J]. 作物学报, 2026, 52(5): 1561-1572.
[13] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[14] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[15] 王宇诚, 张露, 刘阿康, 黄见良, 彭少兵, 袁珅. 基于产量差的作物大面积单产提升策略与展望[J]. 作物学报, 2026, 52(5): 1279-1290.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!