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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 452-458.doi: 10.3724/SP.J.1006.2009.00452

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Identification of Differentially Expressed Gene in Sugarcane Infected by Ustilago scitaminea

QUE You-Xiong,YANG Zhi-XiaXU Li-Ping,CHEN Ru-Kai   

  1. Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002,China
  • Received:2008-06-27 Revised:2008-09-05 Online:2009-03-12 Published:2008-12-11
  • Contact: XIU Li-Ping

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Abstract:

Sugarcanesmut, caused by Ustilago scitaminea Syd., is a most severe fungal disease resulting in heavy economic losses in sugarcane production. The best way to control the disease is to breed resistant varieties. The objective of this study was to survey the molecular mechanism of sugarcane smut resistance. Twosugarcane varieties infected by smut, consisting of NCo376 with high resistance and F134 with susceptibility, were detected to reveal the differential expression of genes regulating resistance to smut with twelve anchored primers and eight random primers via DDRT-PCR. Seven differentially expressed polymorphic fregments were obtained by coloning, sequencing and semi-quantitative RT-PCR validation. The results of Blast in GenBank showed that they shared high homology (52%–97%) with cytochrome C oxidase (CCO) gene, ribosomal protein gene, NAD-dependent malic enzyme gene, aminotransferase gene, binding protein gene and retrotransposon, respectively. The results of semi-quantitative RT-PCR indicated that while CCO gene expression was regulated by U.sticaminea and salicylic acid (SA), its expression was independent on H2O2. Besides, CCO gene expressed in root, stalk and leaf of sugarcane at a relatively low level. From described above, it was inferred that the synthesisof phytoalexin induced by CCO inhibited the pathogen after infection. The results provided useful information for further understanding the molecular mechanism of sugarcane-smut interaction.

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