Abstract:

There is abundant genetic diversity for the wild rice (Oryza rufipogon Griff.) in Gaozhou of Guangdong in China. They are the precious resources for rice genetic improvement. Two hundred and seventeen materials of Gaozhou wild rice were used for this study. An optimal sampling scale of 20% was determined by combining population classification with systematic cluster selection and confirmed by multiple comparisons. Finally, forty three materials were selected as the applied core collection. The analysis of parameters, such as ratio of phenotype retained, variance of phenotype, Shanno-wavear index and coefficient of variation indicated that the applied core collection well represented the total population in genetic diversity and range of variation. The SSR assay with 34 pairs of SSR primers for this core collection showed that the average number of alleles was 6.879, the average genetic diversity index was up to 0.655, gene heterozygosity was 0.558, and 76.7% of materials tested were different in genotypes. This study has laid down a good foundation for effective study and utilization of Gaozhou wild rice.

Key words: Common wild rice, Applied core collection, Phenotype, Genetic background, SSR