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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1931-1940.doi: 10.3724/SP.J.1006.2010.01931


Effects of Seed Priming on Germination and Seedling Growth of Rice under Water Stress

SUN Yuan-Yuan1,2,SUN Yong-Jian1,WANG Ming-Tian1,2,LI Xu-Yi1,GUO Xiang1,HU Rong1,MA Jun1,*   

  1. 1 Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China; 2 Agrometeorological Center of Sichuan Meteorological Bureau, Chengdu 610071, China
  • Received:2010-06-02 Revised:2010-06-30 Online:2010-11-12 Published:2010-08-30
  • Contact: MA Jun,E-mail: majunp2002@163.com

Abstract: Seeds of four different rice genotypes, Gangyou 527, Yangdao 6, Zhonghan 3 and Nongken 57, were treated with two priming method, H2O and different concentrations of polyethylene glycol (PEG). Nonprimed or primed seeds were germinated under different water stress. The contents of proline, soluble protein (SP), total soluble sugars (SS), malonicdialdehyde (MDA), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) in seeds or stressed seedlings, and seed germination and rice seedling characteristics were determined. The results showed that significantly higher levels of proline and SP and lower levels of SS and the content of MDA in primed seeds were observed as compared with control (nonprimed seeds). Priming accelerated the process of glucose metabolism, improved the activities of PAL, SOD, CAT and POD in stressed different genotypic rice seeds, moreover, priming effects was relatively significant. Rice seeds could be initiated significantly better by the priming of proper PEG concentration than hydropriming. The results showed that the best priming conditions was 20% PEG for hybrid indica rice seeds, and 10%–15% PEG for conventional japonica rice seeds. Normal germination of rice seeds it was inhibited when PEG content was beyond the threshold. Response of hydroprimed seeds or PEG primed seeds on seedlings growth of different genotypic rice under different water stresses. Showed that the proper water stress could activate material metabolism, promote rice seeds germination, and significantly increase kinds of physiological index of seedlings in different genotypes of rice, such as morphological index and protective enzymes after the treatment of proper PEG content, But it went against and serious water stress to inhibited germination..It showed that although seed priming treatment could improve activity of rice seedlings, the coordination ability to external germinating environment by self-regulation was limited. It also indicated that indica rice had greater PEG tolerance than conventional japonica rice, but better priming effects were observed in hybrid indica rice.

Key words: Seed priming, Different rice genotypes, Water stress, Germination, Physiological characteristics

[1]Heydecker W. Germination of an idea: the priming of seeds. University of Nottingham School of Agriculture Report, 1973/1974. pp 50–67
[2]Heydecker W, Coolbear P. Seed treatments for improved performance survey and attempted prognosis. Seed Sci Technol, 1977, 5: 353–425
[3]Harris D, Joshi A K, Sodhi P S. On-farm seed priming in semiarid agriculture development and evaluation in maize, rice, and chickpea in India using participatory methods. Exp Agric, 1999, 35: 15–19
[4]Bradford K J. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience, 1986, 21: 1105–1112
[5]Lü X-H(吕小红), Fu J-R(傅家瑞). Effects of PEG pretreatment in vigor and hardiness on seed vigor of peanut. Acta Scifntiarum Naturalium Universitatis Sunyaatseni (中山大学学报×自然科学版), 1990, 29(1): 63–70 (in Chinese with English abstract)
[6]Liao X-R(廖祥儒), Zhu X-C(朱新产). Effect of seed priming on improving resistance of wheat to osmotic stress. Chin Bull Bot (植物学通报), 1997, 14(2): 36–40 (in Chinese with English abstract)
[7]Wang Y-R(王彦荣), Zhang J-Q(张建全), Liu H-X(刘慧霞), Hu X-W(胡小文). Physiological and ecological responses of alfalfa and milkvetch seed to PEG priming. Acta Ecol Sin (生态学报), 2004, 24(3): 402–408 (in Chinese with English abstract)
[8]Sivritepe H O, Eris A, Sivritepe N. The effect of NaCl priming on salt tolerance in melon seedlings. Acta Hort, 1999, 492: 77–84
[9]Ruan S-L(阮松林), Xue Q-Z(薛庆中), Wang Q-H(王清华). Physiological effects of seed priming on salt-tolerance of seedlings in hybrid rice (Oryza sativa L.). Sci Agric Sin (中国农业科学), 2003, 36 (4): 463–468 (in Chinese with English abstract)
[10]Wang X(王熹), Shen B(沈波). Effect of MET-soaked seed on drought resistance of rice seedlings. Acta Phytophysiol Sin (植物生理学报), 1991, 17(1): 105–108 (in Chinese with English abstract)
[11]Chou X-R(瘳祥儒), Sun Q(孙群). Cobaltous ion inhibits the inducement of mungbean draught resistance by seed priming. Seed (种子), 1994, (3): 29–32 (in Chinese with English abstract)
[12]Mittal R, Dubey R S. Influence of sodium chloride salinity on polyphenol oxidase, indole 3-acetic acid oxidase and catalase activities in rice seedlings differing in salt tolerance. Tropical Sci, 1995, 35: 141–149
[13]Bohnert H J, Shen B. Transformation and compatible solutes. Sci Hort, 1999, 78: 237–260
[14]An Y-P(安永平), Qiang A-L(强爱玲), Zhang Y-Y(张媛媛), Zhang W-Y(张文银), Cao G-L(曹桂兰), Han L-Z(韩龙植). Study on characteristics of germination and drought-resistance index by osmotic stress in rice. J Plant Genet Resour (植物遗传资源学报), 2006, 7(4): 421–426 (in Chinese with English abstract)
[15]Wang H-Z(王贺正), Ma J(马均), Li X-Y(李旭毅), Zhang R-P(张荣萍), Li Y(李艳). Study on drought resistance and screening of the rought resistance assessment indexes at germinating stage of rice. Southwest China J Agric Sci (西南农业学报), 2004, 17(5): 594–599 (in Chinese with English abstract)
[16]Gu G-P(顾龚平), Wu G-R(吴国荣), Lu C-M(陆长梅), Zhou C-F(周长芳), Zuo J-J(左吉吉), Wei J-C(魏锦城). Effect of PEG on vigour index and active oxygen metabolism in soybean seeds. Chin J Oil Crop Sci (中国油料作物学报), 2000, 22(2): 26–30 (in Chinese with English abstract)
[17]Li J-P(李季平), Gu H-M(古红梅), Wu S-G(吴诗光), Li J-G(李杰刚). Effect of PEG treatment on physiological-biochemical characteristic of sprouting seed in wheat. J Henan Agric Sci (河南农业科学), 2002, (6): 4–6 (in Chinese with English abstract)
[18]Heydecker W, Higgins J, Gulliver R L. Accelerated germination by osmotic seed treatment. Nature, 1973, 246: 42–44
[19]Bi X-H(毕辛华), Dai X-W(戴心维). Seed Science (种子学). Beijing: China Agriculture Press, 1993. pp 60–61 (in Chinese)
[20]Li H-S(李合生). Plant Physiological and Biochemical Principles and Techniques (植物生理生化实验原理和技术). Beijing: China Higher Education Press, 2000 (in Chinese)
[21]Zucker M. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol, 1965, 40: 779–784
[22]Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981, 22: 867–880
[23]Wakamatsu K, Takahama U. Changes in peroxidase activity and in peroxidsae isozymes in carrot callus. Physiol Plant, 1993, 88: 167–171
[24]Zhang X-Z(张宪政). Crop Physiological Research Method (作物生理研究法). Beijing: China Agriculture Press, 1992 (in Chinese)
[25]Duan J(段俊), Liang C-Y(粱承邺), Huang Y-W(黄毓文). Studies on leaf senescence of hybrid rice at flowering and grain formation stage. Acta Phytophysiol Sin (植物生理学报), 1997, 23(2): 139–144 (in Chinese with English abstract)
[26]Liu J(刘杰), Liu G-S(刘公社), Qi D-M(齐冬梅), Li F-F(李芳芳), Wang E-H(汪恩华). Effect of PEG on germination and active oxygen metabolism in wildrye (Leymus chinensis) seeds. Acta Prataculturae Sin (草业学报), 2002, 11(1): 59–64 (in Chinese with English abstract)
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