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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1931-1940.doi: 10.3724/SP.J.1006.2010.01931

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Seed Priming on Germination and Seedling Growth of Rice under Water Stress

SUN Yuan-Yuan1,2,SUN Yong-Jian1,WANG Ming-Tian1,2,LI Xu-Yi1,GUO Xiang1,HU Rong1,MA Jun1,*   

  1. 1 Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China; 2 Agrometeorological Center of Sichuan Meteorological Bureau, Chengdu 610071, China
  • Received:2010-06-02 Revised:2010-06-30 Online:2010-11-12 Published:2010-08-30
  • Contact: MA Jun,E-mail: majunp2002@163.com

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Abstract: Seeds of four different rice genotypes, Gangyou 527, Yangdao 6, Zhonghan 3 and Nongken 57, were treated with two priming method, H2O and different concentrations of polyethylene glycol (PEG). Nonprimed or primed seeds were germinated under different water stress. The contents of proline, soluble protein (SP), total soluble sugars (SS), malonicdialdehyde (MDA), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) in seeds or stressed seedlings, and seed germination and rice seedling characteristics were determined. The results showed that significantly higher levels of proline and SP and lower levels of SS and the content of MDA in primed seeds were observed as compared with control (nonprimed seeds). Priming accelerated the process of glucose metabolism, improved the activities of PAL, SOD, CAT and POD in stressed different genotypic rice seeds, moreover, priming effects was relatively significant. Rice seeds could be initiated significantly better by the priming of proper PEG concentration than hydropriming. The results showed that the best priming conditions was 20% PEG for hybrid indica rice seeds, and 10%–15% PEG for conventional japonica rice seeds. Normal germination of rice seeds it was inhibited when PEG content was beyond the threshold. Response of hydroprimed seeds or PEG primed seeds on seedlings growth of different genotypic rice under different water stresses. Showed that the proper water stress could activate material metabolism, promote rice seeds germination, and significantly increase kinds of physiological index of seedlings in different genotypes of rice, such as morphological index and protective enzymes after the treatment of proper PEG content, But it went against and serious water stress to inhibited germination..It showed that although seed priming treatment could improve activity of rice seedlings, the coordination ability to external germinating environment by self-regulation was limited. It also indicated that indica rice had greater PEG tolerance than conventional japonica rice, but better priming effects were observed in hybrid indica rice.

Key words: Seed priming, Different rice genotypes, Water stress, Germination, Physiological characteristics

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